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Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques

Blood clotting has become one of the most dangerous side effects associated with Corona virus, as well as the high level of cholesterol and triglycerides in the blood. Therefore, it has become necessary to use medicinal plants that are biologically safe and containing anti-clotting compound. Feijoa...

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Autores principales: Amer, Asmaa A., Elgohary, Rania, Ibrahim, Faten M., Taha, Hussein S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161586/
https://www.ncbi.nlm.nih.gov/pubmed/37151701
http://dx.doi.org/10.1016/j.heliyon.2023.e15444
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author Amer, Asmaa A.
Elgohary, Rania
Ibrahim, Faten M.
Taha, Hussein S.
author_facet Amer, Asmaa A.
Elgohary, Rania
Ibrahim, Faten M.
Taha, Hussein S.
author_sort Amer, Asmaa A.
collection PubMed
description Blood clotting has become one of the most dangerous side effects associated with Corona virus, as well as the high level of cholesterol and triglycerides in the blood. Therefore, it has become necessary to use medicinal plants that are biologically safe and containing anti-clotting compound. Feijoa sellowiana represents a prolific source diverse compounds that may have thrombolytic activity. Therefore, the main research point is the production and scaling up of a target contents that have anticoagulants by using biotechnological techniques; calli production, and bioreactors and assessed their activity through in-vivo study. Murashige and Skoog (MS) medium enriched with varying concentrations of benzyl adenine (BA) and naphthalene acetic acid (NAA) was used to cultivate calli and cell suspension cultures from F. sellowiana seeds. Bioreactors were employed to boost active constituent's production. Moreover, the bioreactor physical factors such as effect of controlled or uncontrolled pH medium were investigated. The leaves of the main plant were extracted by ethanol 70% and polar and non-polar extracts were also prepared. The ethanol extract of calli and cells resulting from bioreactors were also prepared. All prepared extracts were subjected to chemical analysis by HPLC, in-vitro antioxidant assays, in-vivo anticoagulant activity and histopathological examination. Calli and cell suspension cultures were produced by using MS medium fortified with 1 mg/L BA+ 0.1 mg/L NAA. It was found that culturing of cell cultures in a bioreactor with uncontrolled pH and aeration at the value of 0.5 L/min gave the maximum and economical fresh and dry weights of the plants. After evaluation of all extracts; it was found that the calli ethanol extract for each plant was the highest value of total phenolic and total flavonoid contents either quantitatively or qualitatively. All extracts of Feijoa had antioxidant activity. The IC(50) of the DPPH of Feijoa calli extract was 13.45 μg/mL, it was also confirmed by FRAP and ABTs values. Feijoa calli extract decreased platelet aggregation by suppression of thrombin, extended aPTT, PT, bleeding and clotting times. It was safer than warfarin medication. From these findings the authors can conclude that Feijoa had highly anticoagulant activity and the calli production achieved the goal of the enhancement of the phenolic constituent and thus their activity.
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spelling pubmed-101615862023-05-06 Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques Amer, Asmaa A. Elgohary, Rania Ibrahim, Faten M. Taha, Hussein S. Heliyon Research Article Blood clotting has become one of the most dangerous side effects associated with Corona virus, as well as the high level of cholesterol and triglycerides in the blood. Therefore, it has become necessary to use medicinal plants that are biologically safe and containing anti-clotting compound. Feijoa sellowiana represents a prolific source diverse compounds that may have thrombolytic activity. Therefore, the main research point is the production and scaling up of a target contents that have anticoagulants by using biotechnological techniques; calli production, and bioreactors and assessed their activity through in-vivo study. Murashige and Skoog (MS) medium enriched with varying concentrations of benzyl adenine (BA) and naphthalene acetic acid (NAA) was used to cultivate calli and cell suspension cultures from F. sellowiana seeds. Bioreactors were employed to boost active constituent's production. Moreover, the bioreactor physical factors such as effect of controlled or uncontrolled pH medium were investigated. The leaves of the main plant were extracted by ethanol 70% and polar and non-polar extracts were also prepared. The ethanol extract of calli and cells resulting from bioreactors were also prepared. All prepared extracts were subjected to chemical analysis by HPLC, in-vitro antioxidant assays, in-vivo anticoagulant activity and histopathological examination. Calli and cell suspension cultures were produced by using MS medium fortified with 1 mg/L BA+ 0.1 mg/L NAA. It was found that culturing of cell cultures in a bioreactor with uncontrolled pH and aeration at the value of 0.5 L/min gave the maximum and economical fresh and dry weights of the plants. After evaluation of all extracts; it was found that the calli ethanol extract for each plant was the highest value of total phenolic and total flavonoid contents either quantitatively or qualitatively. All extracts of Feijoa had antioxidant activity. The IC(50) of the DPPH of Feijoa calli extract was 13.45 μg/mL, it was also confirmed by FRAP and ABTs values. Feijoa calli extract decreased platelet aggregation by suppression of thrombin, extended aPTT, PT, bleeding and clotting times. It was safer than warfarin medication. From these findings the authors can conclude that Feijoa had highly anticoagulant activity and the calli production achieved the goal of the enhancement of the phenolic constituent and thus their activity. Elsevier 2023-04-11 /pmc/articles/PMC10161586/ /pubmed/37151701 http://dx.doi.org/10.1016/j.heliyon.2023.e15444 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Amer, Asmaa A.
Elgohary, Rania
Ibrahim, Faten M.
Taha, Hussein S.
Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques
title Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques
title_full Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques
title_fullStr Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques
title_full_unstemmed Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques
title_short Anticoagulant effect of Feijoa sellowiana extracts generated by different biotechnological techniques
title_sort anticoagulant effect of feijoa sellowiana extracts generated by different biotechnological techniques
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161586/
https://www.ncbi.nlm.nih.gov/pubmed/37151701
http://dx.doi.org/10.1016/j.heliyon.2023.e15444
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