Cargando…
Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria
Indigenous bacteria play vital roles in the bioremediation of crude oil polluted soils. The effectiveness of the bioremediation process depends on the tolerance, characteristics and biodiversity of the bacteria isolates. Bacteria strains were isolated from crude-oil polluted sites in different locat...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161795/ https://www.ncbi.nlm.nih.gov/pubmed/37151690 http://dx.doi.org/10.1016/j.heliyon.2023.e15639 |
_version_ | 1785037566794268672 |
---|---|
author | Omenna, Emmanuel Chukwuma Omage, Kingsley Ezaka, Emmanuel Azeke, Marshall Arebojie |
author_facet | Omenna, Emmanuel Chukwuma Omage, Kingsley Ezaka, Emmanuel Azeke, Marshall Arebojie |
author_sort | Omenna, Emmanuel Chukwuma |
collection | PubMed |
description | Indigenous bacteria play vital roles in the bioremediation of crude oil polluted soils. The effectiveness of the bioremediation process depends on the tolerance, characteristics and biodiversity of the bacteria isolates. Bacteria strains were isolated from crude-oil polluted sites in different locations in the southern region of Nigeria namely: Azikoro and Otukpoti (Bayelsa state); Ologbo and Benin (Edo State) and non-polluted soil was collected from Ibadan (Oyo state). Tolerance study was conducted for 96 h s. Isolation and characterization of the most effective isolate from each location was done using cultural, physico-chemical and molecular methods. The tolerance level of the isolates from the different oil-polluted soils and their comparative growth performance on crude oil supplemented media decreases in the order: Azikoro - Ologbo - Otukpoti - Benin. MATS analysis showed that cell surfaces of Azikoro, Ologbo and Otukpoti strains exhibited 58–63 % adhesion to n-hexadecane and are hydrophobic strains while Benin strain possess 38% adhesion to n-hexadecane and are hydrophilic. The cell surfaces of isolates from Azikoro, Ologbo and Otukpoti are highly Lewis-acidic while that from Benin is highly Lewis-basic. Isolates from Benin-3, Ologbo-1, and Otukpoti-1 were shown to be gram positive while that from Azikoro was gram negative. 16S rDNA fingerprinting confirmed the identities of the isolates as follows: Paenalcaligenes suwonesis with accession numbers NR-133804.1 from Azikoro spillage site (93.77%); Lactobacillus nagelii with accession number NR-158108.1 (91.30%) from Benin spillage site; Lactobacillus fermentum with accession number NR-104927.1 (96.70%) from Ologbo and Otukpoti spillage sites. Phylogenetic analysis putatively categorized the isolates from Otukpoti and Ologbo in close association belonging to same homology while Benin isolate is a subgroup. The characteristics and biodiversity of all the isolated bacteria from the regions possibly justifies their involvement in the bioremediation of petroleum hydrocarbons. |
format | Online Article Text |
id | pubmed-10161795 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101617952023-05-06 Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria Omenna, Emmanuel Chukwuma Omage, Kingsley Ezaka, Emmanuel Azeke, Marshall Arebojie Heliyon Research Article Indigenous bacteria play vital roles in the bioremediation of crude oil polluted soils. The effectiveness of the bioremediation process depends on the tolerance, characteristics and biodiversity of the bacteria isolates. Bacteria strains were isolated from crude-oil polluted sites in different locations in the southern region of Nigeria namely: Azikoro and Otukpoti (Bayelsa state); Ologbo and Benin (Edo State) and non-polluted soil was collected from Ibadan (Oyo state). Tolerance study was conducted for 96 h s. Isolation and characterization of the most effective isolate from each location was done using cultural, physico-chemical and molecular methods. The tolerance level of the isolates from the different oil-polluted soils and their comparative growth performance on crude oil supplemented media decreases in the order: Azikoro - Ologbo - Otukpoti - Benin. MATS analysis showed that cell surfaces of Azikoro, Ologbo and Otukpoti strains exhibited 58–63 % adhesion to n-hexadecane and are hydrophobic strains while Benin strain possess 38% adhesion to n-hexadecane and are hydrophilic. The cell surfaces of isolates from Azikoro, Ologbo and Otukpoti are highly Lewis-acidic while that from Benin is highly Lewis-basic. Isolates from Benin-3, Ologbo-1, and Otukpoti-1 were shown to be gram positive while that from Azikoro was gram negative. 16S rDNA fingerprinting confirmed the identities of the isolates as follows: Paenalcaligenes suwonesis with accession numbers NR-133804.1 from Azikoro spillage site (93.77%); Lactobacillus nagelii with accession number NR-158108.1 (91.30%) from Benin spillage site; Lactobacillus fermentum with accession number NR-104927.1 (96.70%) from Ologbo and Otukpoti spillage sites. Phylogenetic analysis putatively categorized the isolates from Otukpoti and Ologbo in close association belonging to same homology while Benin isolate is a subgroup. The characteristics and biodiversity of all the isolated bacteria from the regions possibly justifies their involvement in the bioremediation of petroleum hydrocarbons. Elsevier 2023-04-20 /pmc/articles/PMC10161795/ /pubmed/37151690 http://dx.doi.org/10.1016/j.heliyon.2023.e15639 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Omenna, Emmanuel Chukwuma Omage, Kingsley Ezaka, Emmanuel Azeke, Marshall Arebojie Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria |
title | Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria |
title_full | Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria |
title_fullStr | Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria |
title_full_unstemmed | Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria |
title_short | Tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of Nigeria |
title_sort | tolerance, taxonomic and phylogenetic studies of some bacterial isolates involved in bioremediation of crude oil polluted soil in the southern region of nigeria |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161795/ https://www.ncbi.nlm.nih.gov/pubmed/37151690 http://dx.doi.org/10.1016/j.heliyon.2023.e15639 |
work_keys_str_mv | AT omennaemmanuelchukwuma tolerancetaxonomicandphylogeneticstudiesofsomebacterialisolatesinvolvedinbioremediationofcrudeoilpollutedsoilinthesouthernregionofnigeria AT omagekingsley tolerancetaxonomicandphylogeneticstudiesofsomebacterialisolatesinvolvedinbioremediationofcrudeoilpollutedsoilinthesouthernregionofnigeria AT ezakaemmanuel tolerancetaxonomicandphylogeneticstudiesofsomebacterialisolatesinvolvedinbioremediationofcrudeoilpollutedsoilinthesouthernregionofnigeria AT azekemarshallarebojie tolerancetaxonomicandphylogeneticstudiesofsomebacterialisolatesinvolvedinbioremediationofcrudeoilpollutedsoilinthesouthernregionofnigeria |