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Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats

Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattu...

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Autores principales: Yiğit, Nuri, Duman, Mustafa T., Çetintürk, Derya, Saygılı-Yiğit, Fulya, Çolak, Ercüment, Çolak, Reyhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162036/
https://www.ncbi.nlm.nih.gov/pubmed/37151288
http://dx.doi.org/10.7717/peerj.15055
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author Yiğit, Nuri
Duman, Mustafa T.
Çetintürk, Derya
Saygılı-Yiğit, Fulya
Çolak, Ercüment
Çolak, Reyhan
author_facet Yiğit, Nuri
Duman, Mustafa T.
Çetintürk, Derya
Saygılı-Yiğit, Fulya
Çolak, Ercüment
Çolak, Reyhan
author_sort Yiğit, Nuri
collection PubMed
description Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattus rattus) and brown rats (Rattus norvegicus). In this context, previously recorded Ala21Thr mutation (R. rattus) in Exon 1 region, Ile90Leu mutation (R. rattus, R. norvegicus) in Exon 2 region and Leu120Gln mutation (R. norvegicus) in Exon 3 region were identified as “missense mutations” causing amino acid changes. Ala21Thr mutation was first detected in one specimen of Turkish black rat despite the uncertainty of its relevance to resistance. Ile90Leu mutation accepted as neutral variant was detected in most of black rat specimens. Leu120Gln mutation related to anticoagulant rodenticide resistance was found in only one brown rat specimen. Furthermore, Ser74Asn, Gln77Pro (black rat) and Ser79Pro (brown rat) mutations that cause amino acid changes in the Exon 2 region but unclear whether they cause resistance were identified. In addition, “silent mutations” which do not cause amino acid changes were also defined; these mutations were Arg12Arg mutation in Exon 1 region, His68His, Ser81Ser, Ile82Ile and Leu94Leu mutations in Exon 2 region and Ile107Ile, Thr137Thr, Ala143Ala and Gln152Gln mutations in Exon 3 region. These silent mutations were found in both species except for Ser81Ser which was determined in only brown rats.
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spelling pubmed-101620362023-05-06 Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats Yiğit, Nuri Duman, Mustafa T. Çetintürk, Derya Saygılı-Yiğit, Fulya Çolak, Ercüment Çolak, Reyhan PeerJ Genetics Mutations in Exon 1, 2 and 3 of the vitamin K epoxide reductase complex subunit 1 (Vkorc1) gene are known to lead to anticoagulant rodenticide resistance. In order to investigate their putative resistance in rodenticides, we studied the genetic profile of the Vkorc1 gene in Turkish black rats (Rattus rattus) and brown rats (Rattus norvegicus). In this context, previously recorded Ala21Thr mutation (R. rattus) in Exon 1 region, Ile90Leu mutation (R. rattus, R. norvegicus) in Exon 2 region and Leu120Gln mutation (R. norvegicus) in Exon 3 region were identified as “missense mutations” causing amino acid changes. Ala21Thr mutation was first detected in one specimen of Turkish black rat despite the uncertainty of its relevance to resistance. Ile90Leu mutation accepted as neutral variant was detected in most of black rat specimens. Leu120Gln mutation related to anticoagulant rodenticide resistance was found in only one brown rat specimen. Furthermore, Ser74Asn, Gln77Pro (black rat) and Ser79Pro (brown rat) mutations that cause amino acid changes in the Exon 2 region but unclear whether they cause resistance were identified. In addition, “silent mutations” which do not cause amino acid changes were also defined; these mutations were Arg12Arg mutation in Exon 1 region, His68His, Ser81Ser, Ile82Ile and Leu94Leu mutations in Exon 2 region and Ile107Ile, Thr137Thr, Ala143Ala and Gln152Gln mutations in Exon 3 region. These silent mutations were found in both species except for Ser81Ser which was determined in only brown rats. PeerJ Inc. 2023-05-02 /pmc/articles/PMC10162036/ /pubmed/37151288 http://dx.doi.org/10.7717/peerj.15055 Text en ©2023 Yiğit et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Genetics
Yiğit, Nuri
Duman, Mustafa T.
Çetintürk, Derya
Saygılı-Yiğit, Fulya
Çolak, Ercüment
Çolak, Reyhan
Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
title Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
title_full Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
title_fullStr Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
title_full_unstemmed Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
title_short Vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in Turkish rats
title_sort vkorc1 gene polymorphisms confer resistance to anticoagulant rodenticide in turkish rats
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162036/
https://www.ncbi.nlm.nih.gov/pubmed/37151288
http://dx.doi.org/10.7717/peerj.15055
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