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Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast
Microtubules are ubiquitous cytoskeletal polymers with essential functions in chromosome segregation, intracellular transport, and cellular morphogenesis. End-binding proteins (EBs) form the nodes of intricate microtubule plus-end interaction networks. Which EB binding partners are most critical for...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162426/ https://www.ncbi.nlm.nih.gov/pubmed/36884292 http://dx.doi.org/10.1091/mbc.E23-02-0054 |
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author | Kornakov, Nikolay Westermann, Stefan |
author_facet | Kornakov, Nikolay Westermann, Stefan |
author_sort | Kornakov, Nikolay |
collection | PubMed |
description | Microtubules are ubiquitous cytoskeletal polymers with essential functions in chromosome segregation, intracellular transport, and cellular morphogenesis. End-binding proteins (EBs) form the nodes of intricate microtubule plus-end interaction networks. Which EB binding partners are most critical for cell division and how cells organize a microtubule cytoskeleton in the absence of an EB protein are open questions. Here, we perform a detailed analysis of deletion and point mutants of the budding yeast EB protein Bim1. We demonstrate that Bim1 executes its key mitotic functions as part of two cargo complexes—Bim1-Kar9 in the cytoplasm and Bim1-Bik1-Cik1-Kar3 in the nucleus. The latter complex acts during initial metaphase spindle assembly and supports tension establishment and sister chromatid biorientation. We demonstrate that engineered plus-end targeting of Cik1-Kar3 and overexpression of the microtubule crosslinker Ase1 restore distinct aspects of the bim1Δ spindle phenotype. In addition to defining key Bim1-cargo complexes our study also characterizes redundant mechanisms that allow cells to proliferate in the absence of Bim1. |
format | Online Article Text |
id | pubmed-10162426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-101624262023-06-26 Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast Kornakov, Nikolay Westermann, Stefan Mol Biol Cell Articles Microtubules are ubiquitous cytoskeletal polymers with essential functions in chromosome segregation, intracellular transport, and cellular morphogenesis. End-binding proteins (EBs) form the nodes of intricate microtubule plus-end interaction networks. Which EB binding partners are most critical for cell division and how cells organize a microtubule cytoskeleton in the absence of an EB protein are open questions. Here, we perform a detailed analysis of deletion and point mutants of the budding yeast EB protein Bim1. We demonstrate that Bim1 executes its key mitotic functions as part of two cargo complexes—Bim1-Kar9 in the cytoplasm and Bim1-Bik1-Cik1-Kar3 in the nucleus. The latter complex acts during initial metaphase spindle assembly and supports tension establishment and sister chromatid biorientation. We demonstrate that engineered plus-end targeting of Cik1-Kar3 and overexpression of the microtubule crosslinker Ase1 restore distinct aspects of the bim1Δ spindle phenotype. In addition to defining key Bim1-cargo complexes our study also characterizes redundant mechanisms that allow cells to proliferate in the absence of Bim1. The American Society for Cell Biology 2023-04-11 /pmc/articles/PMC10162426/ /pubmed/36884292 http://dx.doi.org/10.1091/mbc.E23-02-0054 Text en © 2023 Kornakov and Westermann. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License. |
spellingShingle | Articles Kornakov, Nikolay Westermann, Stefan Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast |
title | Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast |
title_full | Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast |
title_fullStr | Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast |
title_full_unstemmed | Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast |
title_short | Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast |
title_sort | systematic analysis of microtubule plus-end networks defines eb-cargo complexes critical for mitosis in budding yeast |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162426/ https://www.ncbi.nlm.nih.gov/pubmed/36884292 http://dx.doi.org/10.1091/mbc.E23-02-0054 |
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