Cargando…

Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota

Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into anti-aging drugs that prolong life and improve health is of particular im...

Descripción completa

Detalles Bibliográficos
Autores principales: Zou, Yuan-Feng, JiZe, Xiao-Ping, Li, Cen-Yu, Zhang, Chao-Wen, Fu, Yu-Ping, Yin, Zhong-Qiong, Li, Yang-Ping, Song, Xu, Li, Li-Xia, Zhao, Xing-Hong, Feng, Bin, Huang, Chao, Ye, Gang, Tang, Hua-Qiao, Li, Ning-Yuan, Chen, Ji, Chen, Xing-Fu, Tian, Meng-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162438/
https://www.ncbi.nlm.nih.gov/pubmed/37153605
http://dx.doi.org/10.3389/fimmu.2023.1159291
_version_ 1785037699192717312
author Zou, Yuan-Feng
JiZe, Xiao-Ping
Li, Cen-Yu
Zhang, Chao-Wen
Fu, Yu-Ping
Yin, Zhong-Qiong
Li, Yang-Ping
Song, Xu
Li, Li-Xia
Zhao, Xing-Hong
Feng, Bin
Huang, Chao
Ye, Gang
Tang, Hua-Qiao
Li, Ning-Yuan
Chen, Ji
Chen, Xing-Fu
Tian, Meng-Liang
author_facet Zou, Yuan-Feng
JiZe, Xiao-Ping
Li, Cen-Yu
Zhang, Chao-Wen
Fu, Yu-Ping
Yin, Zhong-Qiong
Li, Yang-Ping
Song, Xu
Li, Li-Xia
Zhao, Xing-Hong
Feng, Bin
Huang, Chao
Ye, Gang
Tang, Hua-Qiao
Li, Ning-Yuan
Chen, Ji
Chen, Xing-Fu
Tian, Meng-Liang
author_sort Zou, Yuan-Feng
collection PubMed
description Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into anti-aging drugs that prolong life and improve health is of particular importance. In this study, the polysaccharide from stems and leaves of Chuanminshen violaceum was obtained with water extraction and alcohol precipitation, and then separated and purified with DEAE anion exchange chromatography and gel filtration to obtain CVP-AP-I. We gavaged natural aging mice with CVP-AP-I and performed serum biochemical analysis, histological staining, quantitative real-time PCR (qRT-PCR) and ELISA kit assays to analyze inflammation and oxidative stress-related gene and protein expression in tissues, and 16SrRNA to analyze intestinal flora. We found that CVP-AP-I significantly improved oxidative stress and inflammatory responses of the intestine and liver, restored the intestinal immune barrier, and balanced the dysbiosis of intestinal flora. In addition, we revealed the potential mechanism behind CVP-AP-I to improve intestinal and liver function by regulating intestinal flora balance and repairing the intestinal immune barrier to regulate the intestinal-liver axis. Our results indicated that C. violaceum polysaccharides possessed favorable antioxidant, anti-inflammatory and potentially anti-aging effects in vivo.
format Online
Article
Text
id pubmed-10162438
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-101624382023-05-06 Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota Zou, Yuan-Feng JiZe, Xiao-Ping Li, Cen-Yu Zhang, Chao-Wen Fu, Yu-Ping Yin, Zhong-Qiong Li, Yang-Ping Song, Xu Li, Li-Xia Zhao, Xing-Hong Feng, Bin Huang, Chao Ye, Gang Tang, Hua-Qiao Li, Ning-Yuan Chen, Ji Chen, Xing-Fu Tian, Meng-Liang Front Immunol Immunology Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into anti-aging drugs that prolong life and improve health is of particular importance. In this study, the polysaccharide from stems and leaves of Chuanminshen violaceum was obtained with water extraction and alcohol precipitation, and then separated and purified with DEAE anion exchange chromatography and gel filtration to obtain CVP-AP-I. We gavaged natural aging mice with CVP-AP-I and performed serum biochemical analysis, histological staining, quantitative real-time PCR (qRT-PCR) and ELISA kit assays to analyze inflammation and oxidative stress-related gene and protein expression in tissues, and 16SrRNA to analyze intestinal flora. We found that CVP-AP-I significantly improved oxidative stress and inflammatory responses of the intestine and liver, restored the intestinal immune barrier, and balanced the dysbiosis of intestinal flora. In addition, we revealed the potential mechanism behind CVP-AP-I to improve intestinal and liver function by regulating intestinal flora balance and repairing the intestinal immune barrier to regulate the intestinal-liver axis. Our results indicated that C. violaceum polysaccharides possessed favorable antioxidant, anti-inflammatory and potentially anti-aging effects in vivo. Frontiers Media S.A. 2023-04-21 /pmc/articles/PMC10162438/ /pubmed/37153605 http://dx.doi.org/10.3389/fimmu.2023.1159291 Text en Copyright © 2023 Zou, JiZe, Li, Zhang, Fu, Yin, Li, Song, Li, Zhao, Feng, Huang, Ye, Tang, Li, Chen, Chen and Tian https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zou, Yuan-Feng
JiZe, Xiao-Ping
Li, Cen-Yu
Zhang, Chao-Wen
Fu, Yu-Ping
Yin, Zhong-Qiong
Li, Yang-Ping
Song, Xu
Li, Li-Xia
Zhao, Xing-Hong
Feng, Bin
Huang, Chao
Ye, Gang
Tang, Hua-Qiao
Li, Ning-Yuan
Chen, Ji
Chen, Xing-Fu
Tian, Meng-Liang
Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
title Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
title_full Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
title_fullStr Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
title_full_unstemmed Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
title_short Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
title_sort polysaccharide from aerial part of chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162438/
https://www.ncbi.nlm.nih.gov/pubmed/37153605
http://dx.doi.org/10.3389/fimmu.2023.1159291
work_keys_str_mv AT zouyuanfeng polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT jizexiaoping polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT licenyu polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT zhangchaowen polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT fuyuping polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT yinzhongqiong polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT liyangping polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT songxu polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT lilixia polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT zhaoxinghong polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT fengbin polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT huangchao polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT yegang polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT tanghuaqiao polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT liningyuan polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT chenji polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT chenxingfu polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota
AT tianmengliang polysaccharidefromaerialpartofchuanminshenviolaceumalleviatesoxidativestressandinflammatoryresponseinagingmicethroughmodulatingintestinalmicrobiota