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Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota
Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into anti-aging drugs that prolong life and improve health is of particular im...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162438/ https://www.ncbi.nlm.nih.gov/pubmed/37153605 http://dx.doi.org/10.3389/fimmu.2023.1159291 |
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author | Zou, Yuan-Feng JiZe, Xiao-Ping Li, Cen-Yu Zhang, Chao-Wen Fu, Yu-Ping Yin, Zhong-Qiong Li, Yang-Ping Song, Xu Li, Li-Xia Zhao, Xing-Hong Feng, Bin Huang, Chao Ye, Gang Tang, Hua-Qiao Li, Ning-Yuan Chen, Ji Chen, Xing-Fu Tian, Meng-Liang |
author_facet | Zou, Yuan-Feng JiZe, Xiao-Ping Li, Cen-Yu Zhang, Chao-Wen Fu, Yu-Ping Yin, Zhong-Qiong Li, Yang-Ping Song, Xu Li, Li-Xia Zhao, Xing-Hong Feng, Bin Huang, Chao Ye, Gang Tang, Hua-Qiao Li, Ning-Yuan Chen, Ji Chen, Xing-Fu Tian, Meng-Liang |
author_sort | Zou, Yuan-Feng |
collection | PubMed |
description | Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into anti-aging drugs that prolong life and improve health is of particular importance. In this study, the polysaccharide from stems and leaves of Chuanminshen violaceum was obtained with water extraction and alcohol precipitation, and then separated and purified with DEAE anion exchange chromatography and gel filtration to obtain CVP-AP-I. We gavaged natural aging mice with CVP-AP-I and performed serum biochemical analysis, histological staining, quantitative real-time PCR (qRT-PCR) and ELISA kit assays to analyze inflammation and oxidative stress-related gene and protein expression in tissues, and 16SrRNA to analyze intestinal flora. We found that CVP-AP-I significantly improved oxidative stress and inflammatory responses of the intestine and liver, restored the intestinal immune barrier, and balanced the dysbiosis of intestinal flora. In addition, we revealed the potential mechanism behind CVP-AP-I to improve intestinal and liver function by regulating intestinal flora balance and repairing the intestinal immune barrier to regulate the intestinal-liver axis. Our results indicated that C. violaceum polysaccharides possessed favorable antioxidant, anti-inflammatory and potentially anti-aging effects in vivo. |
format | Online Article Text |
id | pubmed-10162438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101624382023-05-06 Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota Zou, Yuan-Feng JiZe, Xiao-Ping Li, Cen-Yu Zhang, Chao-Wen Fu, Yu-Ping Yin, Zhong-Qiong Li, Yang-Ping Song, Xu Li, Li-Xia Zhao, Xing-Hong Feng, Bin Huang, Chao Ye, Gang Tang, Hua-Qiao Li, Ning-Yuan Chen, Ji Chen, Xing-Fu Tian, Meng-Liang Front Immunol Immunology Aging is a biological process of progressive deterioration of physiological functions, which poses a serious threat to individual health and a heavy burden on public health systems. As population aging continues, research into anti-aging drugs that prolong life and improve health is of particular importance. In this study, the polysaccharide from stems and leaves of Chuanminshen violaceum was obtained with water extraction and alcohol precipitation, and then separated and purified with DEAE anion exchange chromatography and gel filtration to obtain CVP-AP-I. We gavaged natural aging mice with CVP-AP-I and performed serum biochemical analysis, histological staining, quantitative real-time PCR (qRT-PCR) and ELISA kit assays to analyze inflammation and oxidative stress-related gene and protein expression in tissues, and 16SrRNA to analyze intestinal flora. We found that CVP-AP-I significantly improved oxidative stress and inflammatory responses of the intestine and liver, restored the intestinal immune barrier, and balanced the dysbiosis of intestinal flora. In addition, we revealed the potential mechanism behind CVP-AP-I to improve intestinal and liver function by regulating intestinal flora balance and repairing the intestinal immune barrier to regulate the intestinal-liver axis. Our results indicated that C. violaceum polysaccharides possessed favorable antioxidant, anti-inflammatory and potentially anti-aging effects in vivo. Frontiers Media S.A. 2023-04-21 /pmc/articles/PMC10162438/ /pubmed/37153605 http://dx.doi.org/10.3389/fimmu.2023.1159291 Text en Copyright © 2023 Zou, JiZe, Li, Zhang, Fu, Yin, Li, Song, Li, Zhao, Feng, Huang, Ye, Tang, Li, Chen, Chen and Tian https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Zou, Yuan-Feng JiZe, Xiao-Ping Li, Cen-Yu Zhang, Chao-Wen Fu, Yu-Ping Yin, Zhong-Qiong Li, Yang-Ping Song, Xu Li, Li-Xia Zhao, Xing-Hong Feng, Bin Huang, Chao Ye, Gang Tang, Hua-Qiao Li, Ning-Yuan Chen, Ji Chen, Xing-Fu Tian, Meng-Liang Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota |
title | Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota |
title_full | Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota |
title_fullStr | Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota |
title_full_unstemmed | Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota |
title_short | Polysaccharide from aerial part of Chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota |
title_sort | polysaccharide from aerial part of chuanminshen violaceum alleviates oxidative stress and inflammatory response in aging mice through modulating intestinal microbiota |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162438/ https://www.ncbi.nlm.nih.gov/pubmed/37153605 http://dx.doi.org/10.3389/fimmu.2023.1159291 |
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