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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses

Non-human primate (NHP)-based model systems faithfully reproduce various viral diseases including Ebola, influenza, AIDS and Zika. However, only a small number of NHP cell lines are available and generation of additional cell lines could help to refine these models. We immortalized rhesus macaque ki...

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Autores principales: Reiter, Stefanie, Gärtner, Sabine, Decker, Katharina, Pöhlmann, Stefan, Winkler, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162512/
https://www.ncbi.nlm.nih.gov/pubmed/37146034
http://dx.doi.org/10.1371/journal.pone.0284048
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author Reiter, Stefanie
Gärtner, Sabine
Decker, Katharina
Pöhlmann, Stefan
Winkler, Michael
author_facet Reiter, Stefanie
Gärtner, Sabine
Decker, Katharina
Pöhlmann, Stefan
Winkler, Michael
author_sort Reiter, Stefanie
collection PubMed
description Non-human primate (NHP)-based model systems faithfully reproduce various viral diseases including Ebola, influenza, AIDS and Zika. However, only a small number of NHP cell lines are available and generation of additional cell lines could help to refine these models. We immortalized rhesus macaque kidney cells by lentiviral transduction with a vector encoding telomerase reverse transcriptase (TERT) and report the generation of three TERT-immortalized cell lines derived from rhesus macaque kidney. Expression of the kidney podocyte marker podoplanin on these cells was demonstrated by flow cytometry. Quantitative real-time PCR (qRT-PCR) was employed to demonstrate induction of MX1 expression upon stimulation with interferon (IFN) or viral infection, suggesting a functional IFN system. Further, the cell lines were susceptible to entry driven by the glycoproteins of vesicular stomatitis virus, influenza A virus, Ebola virus, Nipah virus and Lassa virus as assessed by infection with retroviral pseudotypes. Finally, these cells supported growth of Zika virus and the primate simplexviruses Cercopithecine alphaherpesvirus 2 and Papiine alphaherpesvirus 2. In summary, we developed IFN-responsive rhesus macaque kidney cell lines that allowed entry driven by diverse viral glycoproteins and were permissive to infection with Zika virus and primate simplexviruses. These cell lines will be useful for efforts to analyze viral infections of the kidney in macaque models.
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spelling pubmed-101625122023-05-06 Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses Reiter, Stefanie Gärtner, Sabine Decker, Katharina Pöhlmann, Stefan Winkler, Michael PLoS One Research Article Non-human primate (NHP)-based model systems faithfully reproduce various viral diseases including Ebola, influenza, AIDS and Zika. However, only a small number of NHP cell lines are available and generation of additional cell lines could help to refine these models. We immortalized rhesus macaque kidney cells by lentiviral transduction with a vector encoding telomerase reverse transcriptase (TERT) and report the generation of three TERT-immortalized cell lines derived from rhesus macaque kidney. Expression of the kidney podocyte marker podoplanin on these cells was demonstrated by flow cytometry. Quantitative real-time PCR (qRT-PCR) was employed to demonstrate induction of MX1 expression upon stimulation with interferon (IFN) or viral infection, suggesting a functional IFN system. Further, the cell lines were susceptible to entry driven by the glycoproteins of vesicular stomatitis virus, influenza A virus, Ebola virus, Nipah virus and Lassa virus as assessed by infection with retroviral pseudotypes. Finally, these cells supported growth of Zika virus and the primate simplexviruses Cercopithecine alphaherpesvirus 2 and Papiine alphaherpesvirus 2. In summary, we developed IFN-responsive rhesus macaque kidney cell lines that allowed entry driven by diverse viral glycoproteins and were permissive to infection with Zika virus and primate simplexviruses. These cell lines will be useful for efforts to analyze viral infections of the kidney in macaque models. Public Library of Science 2023-05-05 /pmc/articles/PMC10162512/ /pubmed/37146034 http://dx.doi.org/10.1371/journal.pone.0284048 Text en © 2023 Reiter et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Reiter, Stefanie
Gärtner, Sabine
Decker, Katharina
Pöhlmann, Stefan
Winkler, Michael
Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses
title Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses
title_full Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses
title_fullStr Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses
title_full_unstemmed Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses
title_short Development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses
title_sort development of immortalized rhesus macaque kidney cells supporting infection with a panel of viruses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162512/
https://www.ncbi.nlm.nih.gov/pubmed/37146034
http://dx.doi.org/10.1371/journal.pone.0284048
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