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Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1

Human immunodeficiency virus (HIV) infection is associated with a chronic inflammatory stage and continuous activation of inflammasome pathway. We studied the anti-inflammatory effects of the compound cannabidiol (CBD) in comparison with Δ (9)-tetrahydrocannabinol [Δ(9)-THC] in human microglial cell...

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Autores principales: Yndart Arias, Adriana, Kolishetti, Nagesh, Vashist, Arti, Madepalli, Lakshmana, Llaguno, Lorgeleys, Nair, Madhavan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162654/
https://www.ncbi.nlm.nih.gov/pubmed/37147420
http://dx.doi.org/10.1038/s41598-023-32927-4
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author Yndart Arias, Adriana
Kolishetti, Nagesh
Vashist, Arti
Madepalli, Lakshmana
Llaguno, Lorgeleys
Nair, Madhavan
author_facet Yndart Arias, Adriana
Kolishetti, Nagesh
Vashist, Arti
Madepalli, Lakshmana
Llaguno, Lorgeleys
Nair, Madhavan
author_sort Yndart Arias, Adriana
collection PubMed
description Human immunodeficiency virus (HIV) infection is associated with a chronic inflammatory stage and continuous activation of inflammasome pathway. We studied the anti-inflammatory effects of the compound cannabidiol (CBD) in comparison with Δ (9)-tetrahydrocannabinol [Δ(9)-THC] in human microglial cells (HC69.5) infected with HIV. Our results showed that CBD reduced the production of various inflammatory cytokines and chemokines such as MIF, SERPIN E1, IL-6, IL-8, GM-CSF, MCP-1, CXCL1, CXCL10, and IL-1 β compared to Δ(9)-THC treatment. In addition, CBD led to the deactivation of caspase 1, reduced NLRP3 gene expression which play a crucial role in the inflammasome cascade. Furthermore, CBD significantly reduced the expression of HIV. Our study demonstrated that CBD has anti-inflammatory properties and exhibits significant therapeutic potential against HIV-1 infections and neuroinflammation.
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spelling pubmed-101626542023-05-07 Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1 Yndart Arias, Adriana Kolishetti, Nagesh Vashist, Arti Madepalli, Lakshmana Llaguno, Lorgeleys Nair, Madhavan Sci Rep Article Human immunodeficiency virus (HIV) infection is associated with a chronic inflammatory stage and continuous activation of inflammasome pathway. We studied the anti-inflammatory effects of the compound cannabidiol (CBD) in comparison with Δ (9)-tetrahydrocannabinol [Δ(9)-THC] in human microglial cells (HC69.5) infected with HIV. Our results showed that CBD reduced the production of various inflammatory cytokines and chemokines such as MIF, SERPIN E1, IL-6, IL-8, GM-CSF, MCP-1, CXCL1, CXCL10, and IL-1 β compared to Δ(9)-THC treatment. In addition, CBD led to the deactivation of caspase 1, reduced NLRP3 gene expression which play a crucial role in the inflammasome cascade. Furthermore, CBD significantly reduced the expression of HIV. Our study demonstrated that CBD has anti-inflammatory properties and exhibits significant therapeutic potential against HIV-1 infections and neuroinflammation. Nature Publishing Group UK 2023-05-05 /pmc/articles/PMC10162654/ /pubmed/37147420 http://dx.doi.org/10.1038/s41598-023-32927-4 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yndart Arias, Adriana
Kolishetti, Nagesh
Vashist, Arti
Madepalli, Lakshmana
Llaguno, Lorgeleys
Nair, Madhavan
Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
title Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
title_full Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
title_fullStr Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
title_full_unstemmed Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
title_short Anti-inflammatory effects of CBD in human microglial cell line infected with HIV-1
title_sort anti-inflammatory effects of cbd in human microglial cell line infected with hiv-1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10162654/
https://www.ncbi.nlm.nih.gov/pubmed/37147420
http://dx.doi.org/10.1038/s41598-023-32927-4
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