Principles and advances in ultrafast photoacoustics; applications to imaging cell mechanics and to probing cell nanostructure

In this article we first present the foundations of ultrafast photoacoustics, a technique where the acoustic wavelength in play can be considerably shorter than the optical wavelength. The physics primarily involved in the conversion of short light pulses into high frequency sound is described. The mechanical disturbances following the relaxation of hot electrons in metals and other processes leading to the breaking of the mechanical balance are presented, and the generation of bulk shear-waves, of surface and interface waves and of guided waves is discussed. Then, efforts to overcome the limitations imposed by optical diffraction are described. Next, the principles behind the detection of the so generated coherent acoustic phonons with short light pulses are introduced for both opaque and transparent materials. The striking instrumental advances, in the detection of acoustic displacements, ultrafast acquisition, frequency and space resolution are discussed. Then secondly, we introduce picosecond opto-acoustics as a remote and label-free novel modality with an excellent capacity for quantitative evaluation and imaging of the cell’s mechanical properties, currently with micron in-plane and sub-optical in depth resolution. We present the methods for time domain Brillouin spectroscopy in cells and for cell ultrasonography. The current applications of this unconventional means of addressing biological questions are presented. This microscopy of the nanoscale intra-cell mechanics, based on the optical monitoring of coherent phonons, is currently emerging as a breakthrough method offering new insights into the supra-molecular structural changes that accompany cell response to a myriad of biological events.