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Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne

BACKGROUND: The inflammatory response is an important part of the pathogenesis of acne vulgaris. Auriculotherapy has been shown to have a good therapeutic effect on this disease. The aim of this study was to explore the mechanism underlying the anti-inflammatory effect of auriculotherapy in the trea...

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Autores principales: Zuo, Guang, Gao, Yidan, Lu, Guangtong, Bu, Ming, Liu, Jun, Zhang, Juncha, Fan, Xisheng, Chen, Hao, Wang, Xuesong, She, Yanfen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163966/
https://www.ncbi.nlm.nih.gov/pubmed/37159798
http://dx.doi.org/10.1155/2023/6627393
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author Zuo, Guang
Gao, Yidan
Lu, Guangtong
Bu, Ming
Liu, Jun
Zhang, Juncha
Fan, Xisheng
Chen, Hao
Wang, Xuesong
She, Yanfen
author_facet Zuo, Guang
Gao, Yidan
Lu, Guangtong
Bu, Ming
Liu, Jun
Zhang, Juncha
Fan, Xisheng
Chen, Hao
Wang, Xuesong
She, Yanfen
author_sort Zuo, Guang
collection PubMed
description BACKGROUND: The inflammatory response is an important part of the pathogenesis of acne vulgaris. Auriculotherapy has been shown to have a good therapeutic effect on this disease. The aim of this study was to explore the mechanism underlying the anti-inflammatory effect of auriculotherapy in the treatment of acne vulgaris. METHODS: Propionibacterium acnes was injected subcutaneously into the ears of rats to establish an animal model of acne. The auriculotherapy intervention in rats consisted of auricular bloodletting therapy (ABT), auricular point sticking (APS), or a combination of both (ABPS). The anti-inflammatory effects of auriculotherapy were evaluated by measuring changes in ear thickness, local body surface microcirculation in the ear, and serum inflammatory factors in rats. The polarization of macrophages was analyzed by flow cytometry, and the expression of TLR2/NF-κB signaling pathway in the target tissues was analyzed using western blot. RESULTS: ABT, APS, and ABPS all reduced the erythema of ear acne, decreased microcirculation in localized ear acne, and decreased serum levels of TNF-α and IL-1β in rats. Meanwhile, the three interventions reduced M1-type macrophages and increased M2-type macrophages; only APS could reduce the expression of TLR2/NF-κB signaling pathway. CONCLUSION: ABT, APS, and ABPS can improve the inflammatory symptoms of acne and reduce inflammatory cytokines. APS may exert anti-inflammatory effects by altering macrophage polarization and decreasing TLR2/NF-κB expression.
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spelling pubmed-101639662023-05-07 Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne Zuo, Guang Gao, Yidan Lu, Guangtong Bu, Ming Liu, Jun Zhang, Juncha Fan, Xisheng Chen, Hao Wang, Xuesong She, Yanfen Mediators Inflamm Research Article BACKGROUND: The inflammatory response is an important part of the pathogenesis of acne vulgaris. Auriculotherapy has been shown to have a good therapeutic effect on this disease. The aim of this study was to explore the mechanism underlying the anti-inflammatory effect of auriculotherapy in the treatment of acne vulgaris. METHODS: Propionibacterium acnes was injected subcutaneously into the ears of rats to establish an animal model of acne. The auriculotherapy intervention in rats consisted of auricular bloodletting therapy (ABT), auricular point sticking (APS), or a combination of both (ABPS). The anti-inflammatory effects of auriculotherapy were evaluated by measuring changes in ear thickness, local body surface microcirculation in the ear, and serum inflammatory factors in rats. The polarization of macrophages was analyzed by flow cytometry, and the expression of TLR2/NF-κB signaling pathway in the target tissues was analyzed using western blot. RESULTS: ABT, APS, and ABPS all reduced the erythema of ear acne, decreased microcirculation in localized ear acne, and decreased serum levels of TNF-α and IL-1β in rats. Meanwhile, the three interventions reduced M1-type macrophages and increased M2-type macrophages; only APS could reduce the expression of TLR2/NF-κB signaling pathway. CONCLUSION: ABT, APS, and ABPS can improve the inflammatory symptoms of acne and reduce inflammatory cytokines. APS may exert anti-inflammatory effects by altering macrophage polarization and decreasing TLR2/NF-κB expression. Hindawi 2023-04-29 /pmc/articles/PMC10163966/ /pubmed/37159798 http://dx.doi.org/10.1155/2023/6627393 Text en Copyright © 2023 Guang Zuo et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zuo, Guang
Gao, Yidan
Lu, Guangtong
Bu, Ming
Liu, Jun
Zhang, Juncha
Fan, Xisheng
Chen, Hao
Wang, Xuesong
She, Yanfen
Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne
title Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne
title_full Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne
title_fullStr Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne
title_full_unstemmed Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne
title_short Auriculotherapy Modulates Macrophage Polarization to Reduce Inflammatory Response in a Rat Model of Acne
title_sort auriculotherapy modulates macrophage polarization to reduce inflammatory response in a rat model of acne
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10163966/
https://www.ncbi.nlm.nih.gov/pubmed/37159798
http://dx.doi.org/10.1155/2023/6627393
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