Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei

Lacticaseibacillus paracasei is an economically important bacterial species, used in the food industry and as a probiotic. Here, we investigate the roles of N6-methyladenine (6mA) modification in L. paracasei using multi-omics and high-throughput chromosome conformation capture (Hi-C) analyses. The...

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Autores principales: Zhao, Jie, Zhang, Meng, Hui, Wenyan, Zhang, Yue, Wang, Jing, Wang, Shaojing, Kwok, Lai-Yu, Kong, Jian, Zhang, Heping, Zhang, Wenyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164179/
https://www.ncbi.nlm.nih.gov/pubmed/37149616
http://dx.doi.org/10.1038/s41467-023-38291-1
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author Zhao, Jie
Zhang, Meng
Hui, Wenyan
Zhang, Yue
Wang, Jing
Wang, Shaojing
Kwok, Lai-Yu
Kong, Jian
Zhang, Heping
Zhang, Wenyi
author_facet Zhao, Jie
Zhang, Meng
Hui, Wenyan
Zhang, Yue
Wang, Jing
Wang, Shaojing
Kwok, Lai-Yu
Kong, Jian
Zhang, Heping
Zhang, Wenyi
author_sort Zhao, Jie
collection PubMed
description Lacticaseibacillus paracasei is an economically important bacterial species, used in the food industry and as a probiotic. Here, we investigate the roles of N6-methyladenine (6mA) modification in L. paracasei using multi-omics and high-throughput chromosome conformation capture (Hi-C) analyses. The distribution of 6mA-modified sites varies across the genomes of 28 strains, and appears to be enriched near genes involved in carbohydrate metabolism. A pglX mutant, defective in 6mA modification, shows transcriptomic alterations but only modest changes in growth and genomic spatial organization.
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spelling pubmed-101641792023-05-08 Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei Zhao, Jie Zhang, Meng Hui, Wenyan Zhang, Yue Wang, Jing Wang, Shaojing Kwok, Lai-Yu Kong, Jian Zhang, Heping Zhang, Wenyi Nat Commun Article Lacticaseibacillus paracasei is an economically important bacterial species, used in the food industry and as a probiotic. Here, we investigate the roles of N6-methyladenine (6mA) modification in L. paracasei using multi-omics and high-throughput chromosome conformation capture (Hi-C) analyses. The distribution of 6mA-modified sites varies across the genomes of 28 strains, and appears to be enriched near genes involved in carbohydrate metabolism. A pglX mutant, defective in 6mA modification, shows transcriptomic alterations but only modest changes in growth and genomic spatial organization. Nature Publishing Group UK 2023-05-06 /pmc/articles/PMC10164179/ /pubmed/37149616 http://dx.doi.org/10.1038/s41467-023-38291-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Zhao, Jie
Zhang, Meng
Hui, Wenyan
Zhang, Yue
Wang, Jing
Wang, Shaojing
Kwok, Lai-Yu
Kong, Jian
Zhang, Heping
Zhang, Wenyi
Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei
title Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei
title_full Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei
title_fullStr Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei
title_full_unstemmed Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei
title_short Roles of adenine methylation in the physiology of Lacticaseibacillus paracasei
title_sort roles of adenine methylation in the physiology of lacticaseibacillus paracasei
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10164179/
https://www.ncbi.nlm.nih.gov/pubmed/37149616
http://dx.doi.org/10.1038/s41467-023-38291-1
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