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Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5

Phenazine-1-carboxylic acid (PCA) is a biologically active substance with the ability to prevent and control crop diseases. It was certified as a pesticide by the Ministry of Agriculture of China in 2011 and was named “Shenzimycin.” Lzh-T5 is a Pseudomonas chlororaphis strain found in the rhizospher...

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Autores principales: Liu, Kaiquan, Li, Zhenghua, Liang, Xiaoli, Xu, Yanpeng, Cao, Yufei, Wang, Ruiming, Li, Piwu, Li, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165110/
https://www.ncbi.nlm.nih.gov/pubmed/37168109
http://dx.doi.org/10.3389/fmicb.2023.1186052
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author Liu, Kaiquan
Li, Zhenghua
Liang, Xiaoli
Xu, Yanpeng
Cao, Yufei
Wang, Ruiming
Li, Piwu
Li, Ling
author_facet Liu, Kaiquan
Li, Zhenghua
Liang, Xiaoli
Xu, Yanpeng
Cao, Yufei
Wang, Ruiming
Li, Piwu
Li, Ling
author_sort Liu, Kaiquan
collection PubMed
description Phenazine-1-carboxylic acid (PCA) is a biologically active substance with the ability to prevent and control crop diseases. It was certified as a pesticide by the Ministry of Agriculture of China in 2011 and was named “Shenzimycin.” Lzh-T5 is a Pseudomonas chlororaphis strain found in the rhizosphere of tomatoes. This strain can produce only 230 mg/L of PCA. We used LDA-4, which produces the phenazine synthetic intermediate trans-2,3-dihydro-3-hydroxyanthranilic acid in high amounts, as the starting strain. By restoring phzF and knocking out phzO, we achieved PCA accumulation. Moreover, PCA production was enhanced after knocking out negative regulators, enhancing the shikimate pathway, and performing fed-batch fermentation, thus resulting in the production of 10,653 mg/L of PCA. It suggested that P. chlororaphis Lzh-T5 has the potential to become an efficiency cell factory of biologically active substances.
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spelling pubmed-101651102023-05-09 Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5 Liu, Kaiquan Li, Zhenghua Liang, Xiaoli Xu, Yanpeng Cao, Yufei Wang, Ruiming Li, Piwu Li, Ling Front Microbiol Microbiology Phenazine-1-carboxylic acid (PCA) is a biologically active substance with the ability to prevent and control crop diseases. It was certified as a pesticide by the Ministry of Agriculture of China in 2011 and was named “Shenzimycin.” Lzh-T5 is a Pseudomonas chlororaphis strain found in the rhizosphere of tomatoes. This strain can produce only 230 mg/L of PCA. We used LDA-4, which produces the phenazine synthetic intermediate trans-2,3-dihydro-3-hydroxyanthranilic acid in high amounts, as the starting strain. By restoring phzF and knocking out phzO, we achieved PCA accumulation. Moreover, PCA production was enhanced after knocking out negative regulators, enhancing the shikimate pathway, and performing fed-batch fermentation, thus resulting in the production of 10,653 mg/L of PCA. It suggested that P. chlororaphis Lzh-T5 has the potential to become an efficiency cell factory of biologically active substances. Frontiers Media S.A. 2023-04-24 /pmc/articles/PMC10165110/ /pubmed/37168109 http://dx.doi.org/10.3389/fmicb.2023.1186052 Text en Copyright © 2023 Liu, Li, Liang, Xu, Cao, Wang, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Kaiquan
Li, Zhenghua
Liang, Xiaoli
Xu, Yanpeng
Cao, Yufei
Wang, Ruiming
Li, Piwu
Li, Ling
Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5
title Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5
title_full Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5
title_fullStr Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5
title_full_unstemmed Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5
title_short Biosynthesis and genetic engineering of phenazine-1-carboxylic acid in Pseudomonas chlororaphis Lzh-T5
title_sort biosynthesis and genetic engineering of phenazine-1-carboxylic acid in pseudomonas chlororaphis lzh-t5
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165110/
https://www.ncbi.nlm.nih.gov/pubmed/37168109
http://dx.doi.org/10.3389/fmicb.2023.1186052
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