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Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge

Intestinal integrity losses have been identified as a main driver for poor performance in broilers. The oral administration of markers such as iohexol is a major asset for measuring intestinal permeability (IP) alterations. The aim of the current study was to evaluate oral iohexol administration and...

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Autores principales: Rysman, Katrien, Eeckhaut, Venessa, Croubels, Siska, Maertens, Brecht, Van Immerseel, Filip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165152/
https://www.ncbi.nlm.nih.gov/pubmed/37099878
http://dx.doi.org/10.1016/j.psj.2023.102690
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author Rysman, Katrien
Eeckhaut, Venessa
Croubels, Siska
Maertens, Brecht
Van Immerseel, Filip
author_facet Rysman, Katrien
Eeckhaut, Venessa
Croubels, Siska
Maertens, Brecht
Van Immerseel, Filip
author_sort Rysman, Katrien
collection PubMed
description Intestinal integrity losses have been identified as a main driver for poor performance in broilers. The oral administration of markers such as iohexol is a major asset for measuring intestinal permeability (IP) alterations. The aim of the current study was to evaluate oral iohexol administration and serum levels as a quantitative measure for IP in Ross 308 broilers and to identify possible associations with histologic measurements. A total of 40, day-old broiler chickens were randomly divided into 4 groups of 10 broilers and a coccidiosis model was used to induce IP. Three challenge groups received a mixture of different field strains and concentrations of Eimeria acervulina and Eimeria maxima at d 16, and 1 group operated as an uninfected control group. On d 20, 5 birds per group were orally administered the permeability marker iohexol at a dose of 64.7 mg/kg body weight and blood was taken 60 min after the oral gavage. On d 21 these 5 birds per group were euthanized. On d 21, 5 other birds per group were given iohexol where after blood was taken. These birds were euthanized on d 22. During necropsy, birds were scored for coccidiosis lesions and a duodenal segment was taken for histology. The Eimeria challenge had a significant impact on the villus length, crypt depth, villus-to-crypt ratio and CD3(+) T-lymphocytes area percentage. Challenged birds had a significant higher concentration of serum iohexol on both sampling days, as compared to the uninfected controls. A significant correlation could be found between the serum iohexol concentration and the histologic parameters (villus length, crypt depth and villus-to-crypt ratio) on the first sampling day. This suggests that iohexol may be used as a gut permeability marker in broilers under Eimeria challenge.
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spelling pubmed-101651522023-05-09 Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge Rysman, Katrien Eeckhaut, Venessa Croubels, Siska Maertens, Brecht Van Immerseel, Filip Poult Sci IMMUNOLOGY, HEALTH AND DISEASE Intestinal integrity losses have been identified as a main driver for poor performance in broilers. The oral administration of markers such as iohexol is a major asset for measuring intestinal permeability (IP) alterations. The aim of the current study was to evaluate oral iohexol administration and serum levels as a quantitative measure for IP in Ross 308 broilers and to identify possible associations with histologic measurements. A total of 40, day-old broiler chickens were randomly divided into 4 groups of 10 broilers and a coccidiosis model was used to induce IP. Three challenge groups received a mixture of different field strains and concentrations of Eimeria acervulina and Eimeria maxima at d 16, and 1 group operated as an uninfected control group. On d 20, 5 birds per group were orally administered the permeability marker iohexol at a dose of 64.7 mg/kg body weight and blood was taken 60 min after the oral gavage. On d 21 these 5 birds per group were euthanized. On d 21, 5 other birds per group were given iohexol where after blood was taken. These birds were euthanized on d 22. During necropsy, birds were scored for coccidiosis lesions and a duodenal segment was taken for histology. The Eimeria challenge had a significant impact on the villus length, crypt depth, villus-to-crypt ratio and CD3(+) T-lymphocytes area percentage. Challenged birds had a significant higher concentration of serum iohexol on both sampling days, as compared to the uninfected controls. A significant correlation could be found between the serum iohexol concentration and the histologic parameters (villus length, crypt depth and villus-to-crypt ratio) on the first sampling day. This suggests that iohexol may be used as a gut permeability marker in broilers under Eimeria challenge. Elsevier 2023-03-31 /pmc/articles/PMC10165152/ /pubmed/37099878 http://dx.doi.org/10.1016/j.psj.2023.102690 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle IMMUNOLOGY, HEALTH AND DISEASE
Rysman, Katrien
Eeckhaut, Venessa
Croubels, Siska
Maertens, Brecht
Van Immerseel, Filip
Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
title Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
title_full Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
title_fullStr Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
title_full_unstemmed Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
title_short Iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
title_sort iohexol is an intestinal permeability marker in broilers under coccidiosis challenge
topic IMMUNOLOGY, HEALTH AND DISEASE
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165152/
https://www.ncbi.nlm.nih.gov/pubmed/37099878
http://dx.doi.org/10.1016/j.psj.2023.102690
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