Protocol to screen for Sorafenib resistance regulators using pooled lentiviral shRNA library and a Sorafenib-resistant hepatocellular carcinoma cell model

Approaches to study therapy resistance in HCC (hepatocellular carcinoma) are limited, especially when using HCC models in vitro. Here, we present a protocol to establish an in vitro Sorafenib-resistant human HCC cell model and conduct an shRNA-mediated synthetic lethal screen in established Sorafeni...

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Detalles Bibliográficos
Autores principales: Gao, Ruize, Tang, Fengyuan, Christofori, Gerhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165445/
https://www.ncbi.nlm.nih.gov/pubmed/37126443
http://dx.doi.org/10.1016/j.xpro.2023.102273

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