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Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector

Tick microbiota can be targeted for the control of tick-borne diseases such as human granulocytic anaplasmosis (HGA) caused by model pathogen, Anaplasma phagocytophilum. Frankenbacteriosis is inspired by Frankenstein and defined here as paratransgenesis of tick symbiotic/commensal bacteria to mimic...

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Autores principales: Mazuecos, Lorena, Alberdi, Pilar, Hernández-Jarguín, Angélica, Contreras, Marinela, Villar, Margarita, Cabezas-Cruz, Alejandro, Simo, Ladislav, González-García, Almudena, Díaz-Sánchez, Sandra, Neelakanta, Girish, Bonnet, Sarah I., Fikrig, Erol, de la Fuente, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165458/
https://www.ncbi.nlm.nih.gov/pubmed/37168564
http://dx.doi.org/10.1016/j.isci.2023.106697
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author Mazuecos, Lorena
Alberdi, Pilar
Hernández-Jarguín, Angélica
Contreras, Marinela
Villar, Margarita
Cabezas-Cruz, Alejandro
Simo, Ladislav
González-García, Almudena
Díaz-Sánchez, Sandra
Neelakanta, Girish
Bonnet, Sarah I.
Fikrig, Erol
de la Fuente, José
author_facet Mazuecos, Lorena
Alberdi, Pilar
Hernández-Jarguín, Angélica
Contreras, Marinela
Villar, Margarita
Cabezas-Cruz, Alejandro
Simo, Ladislav
González-García, Almudena
Díaz-Sánchez, Sandra
Neelakanta, Girish
Bonnet, Sarah I.
Fikrig, Erol
de la Fuente, José
author_sort Mazuecos, Lorena
collection PubMed
description Tick microbiota can be targeted for the control of tick-borne diseases such as human granulocytic anaplasmosis (HGA) caused by model pathogen, Anaplasma phagocytophilum. Frankenbacteriosis is inspired by Frankenstein and defined here as paratransgenesis of tick symbiotic/commensal bacteria to mimic and compete with tick-borne pathogens. Interactions between A. phagocytophilum and symbiotic Sphingomonas identified by metaproteomics analysis in Ixodes scapularis midgut showed competition between both bacteria. Consequently, Sphingomonas was selected for frankenbacteriosis for the control of A. phagocytophilum infection and transmission. The results showed that Franken Sphingomonas producing A. phagocytophilum major surface protein 4 (MSP4) mimic pathogen and reduce infection in ticks by competition and interaction with cell receptor components of infection. Franken Sphingomonas-MSP4 transovarial and trans-stadial transmission suggests that tick larvae with genetically modified Franken Sphingomonas-MSP4 could be produced in the laboratory and released in the field to compete and replace the wildtype populations with associated reduction in pathogen infection/transmission and HGA disease risks.
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spelling pubmed-101654582023-05-09 Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector Mazuecos, Lorena Alberdi, Pilar Hernández-Jarguín, Angélica Contreras, Marinela Villar, Margarita Cabezas-Cruz, Alejandro Simo, Ladislav González-García, Almudena Díaz-Sánchez, Sandra Neelakanta, Girish Bonnet, Sarah I. Fikrig, Erol de la Fuente, José iScience Article Tick microbiota can be targeted for the control of tick-borne diseases such as human granulocytic anaplasmosis (HGA) caused by model pathogen, Anaplasma phagocytophilum. Frankenbacteriosis is inspired by Frankenstein and defined here as paratransgenesis of tick symbiotic/commensal bacteria to mimic and compete with tick-borne pathogens. Interactions between A. phagocytophilum and symbiotic Sphingomonas identified by metaproteomics analysis in Ixodes scapularis midgut showed competition between both bacteria. Consequently, Sphingomonas was selected for frankenbacteriosis for the control of A. phagocytophilum infection and transmission. The results showed that Franken Sphingomonas producing A. phagocytophilum major surface protein 4 (MSP4) mimic pathogen and reduce infection in ticks by competition and interaction with cell receptor components of infection. Franken Sphingomonas-MSP4 transovarial and trans-stadial transmission suggests that tick larvae with genetically modified Franken Sphingomonas-MSP4 could be produced in the laboratory and released in the field to compete and replace the wildtype populations with associated reduction in pathogen infection/transmission and HGA disease risks. Elsevier 2023-04-18 /pmc/articles/PMC10165458/ /pubmed/37168564 http://dx.doi.org/10.1016/j.isci.2023.106697 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Mazuecos, Lorena
Alberdi, Pilar
Hernández-Jarguín, Angélica
Contreras, Marinela
Villar, Margarita
Cabezas-Cruz, Alejandro
Simo, Ladislav
González-García, Almudena
Díaz-Sánchez, Sandra
Neelakanta, Girish
Bonnet, Sarah I.
Fikrig, Erol
de la Fuente, José
Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
title Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
title_full Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
title_fullStr Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
title_full_unstemmed Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
title_short Frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
title_sort frankenbacteriosis targeting interactions between pathogen and symbiont to control infection in the tick vector
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10165458/
https://www.ncbi.nlm.nih.gov/pubmed/37168564
http://dx.doi.org/10.1016/j.isci.2023.106697
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