Cargando…

Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex

Oxidative stress response is a fundamental biological process mediated by conserved mechanisms. The identities and functions of some key regulators remain unknown. Here, we report a novel role of C. elegans casein kinase 1 gamma CSNK-1 (also known as CK1γ or CSNK1G) in regulating oxidative stress re...

Descripción completa

Detalles Bibliográficos
Autores principales: Hu, Yiman, Xu, Zhaofa, Pan, Qian, Ma, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166522/
https://www.ncbi.nlm.nih.gov/pubmed/37099597
http://dx.doi.org/10.1371/journal.pgen.1010740
_version_ 1785038460859449344
author Hu, Yiman
Xu, Zhaofa
Pan, Qian
Ma, Long
author_facet Hu, Yiman
Xu, Zhaofa
Pan, Qian
Ma, Long
author_sort Hu, Yiman
collection PubMed
description Oxidative stress response is a fundamental biological process mediated by conserved mechanisms. The identities and functions of some key regulators remain unknown. Here, we report a novel role of C. elegans casein kinase 1 gamma CSNK-1 (also known as CK1γ or CSNK1G) in regulating oxidative stress response and ROS levels. csnk-1 interacted with the bli-3/tsp-15/doxa-1 NADPH dual oxidase genes via genetic nonallelic noncomplementation to affect C. elegans survival in oxidative stress. The genetic interaction was supported by specific biochemical interactions between DOXA-1 and CSNK-1 and potentially between their human orthologs DUOXA2 and CSNK1G2. Consistently, CSNK-1 was required for normal ROS levels in C. elegans. CSNK1G2 and DUOXA2 each can promote ROS levels in human cells, effects that were suppressed by a small molecule casein kinase 1 inhibitor. We also detected genetic interactions between csnk-1 and skn-1 Nrf2 in oxidative stress response. Together, we propose that CSNK-1 CSNK1G defines a novel conserved regulatory mechanism for ROS homeostasis.
format Online
Article
Text
id pubmed-10166522
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-101665222023-05-09 Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex Hu, Yiman Xu, Zhaofa Pan, Qian Ma, Long PLoS Genet Research Article Oxidative stress response is a fundamental biological process mediated by conserved mechanisms. The identities and functions of some key regulators remain unknown. Here, we report a novel role of C. elegans casein kinase 1 gamma CSNK-1 (also known as CK1γ or CSNK1G) in regulating oxidative stress response and ROS levels. csnk-1 interacted with the bli-3/tsp-15/doxa-1 NADPH dual oxidase genes via genetic nonallelic noncomplementation to affect C. elegans survival in oxidative stress. The genetic interaction was supported by specific biochemical interactions between DOXA-1 and CSNK-1 and potentially between their human orthologs DUOXA2 and CSNK1G2. Consistently, CSNK-1 was required for normal ROS levels in C. elegans. CSNK1G2 and DUOXA2 each can promote ROS levels in human cells, effects that were suppressed by a small molecule casein kinase 1 inhibitor. We also detected genetic interactions between csnk-1 and skn-1 Nrf2 in oxidative stress response. Together, we propose that CSNK-1 CSNK1G defines a novel conserved regulatory mechanism for ROS homeostasis. Public Library of Science 2023-04-26 /pmc/articles/PMC10166522/ /pubmed/37099597 http://dx.doi.org/10.1371/journal.pgen.1010740 Text en © 2023 Hu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hu, Yiman
Xu, Zhaofa
Pan, Qian
Ma, Long
Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex
title Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex
title_full Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex
title_fullStr Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex
title_full_unstemmed Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex
title_short Casein kinase 1 gamma regulates oxidative stress response via interacting with the NADPH dual oxidase complex
title_sort casein kinase 1 gamma regulates oxidative stress response via interacting with the nadph dual oxidase complex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166522/
https://www.ncbi.nlm.nih.gov/pubmed/37099597
http://dx.doi.org/10.1371/journal.pgen.1010740
work_keys_str_mv AT huyiman caseinkinase1gammaregulatesoxidativestressresponseviainteractingwiththenadphdualoxidasecomplex
AT xuzhaofa caseinkinase1gammaregulatesoxidativestressresponseviainteractingwiththenadphdualoxidasecomplex
AT panqian caseinkinase1gammaregulatesoxidativestressresponseviainteractingwiththenadphdualoxidasecomplex
AT malong caseinkinase1gammaregulatesoxidativestressresponseviainteractingwiththenadphdualoxidasecomplex