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IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway
Immune status including the immune cells and cytokine profiles has been implicated in the development of endometriosis. In this study, we analyzed Th17 cells and IL-17A in peritoneal fluid (PF) and endometrial tissues of patients with (n=10) and without (n=26) endometriosis. Our study has shown incr...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Association of Immunologists
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166657/ https://www.ncbi.nlm.nih.gov/pubmed/37179747 http://dx.doi.org/10.4110/in.2023.23.e14 |
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author | Kang, Young-Ju Cho, Hee Jun Lee, Yunhee Park, Arum Kim, Mi Jeong Jeung, In Cheul Jung, Yong-Wook Jung, Haiyoung Choi, Inpyo Lee, Hee Gu Yoon, Suk Ran |
author_facet | Kang, Young-Ju Cho, Hee Jun Lee, Yunhee Park, Arum Kim, Mi Jeong Jeung, In Cheul Jung, Yong-Wook Jung, Haiyoung Choi, Inpyo Lee, Hee Gu Yoon, Suk Ran |
author_sort | Kang, Young-Ju |
collection | PubMed |
description | Immune status including the immune cells and cytokine profiles has been implicated in the development of endometriosis. In this study, we analyzed Th17 cells and IL-17A in peritoneal fluid (PF) and endometrial tissues of patients with (n=10) and without (n=26) endometriosis. Our study has shown increased Th17 cell population and IL-17A level in PF with endometriosis patients. To determine the roles of IL-17A and Th17 cells in the development of endometriosis, the effect of IL-17A, major cytokine of Th17, on endometrial cells isolated from endometriotic tissues was examined. Recombinant IL-17A promoted survival of endometrial cells accompanied by increased expression of anti-apoptotic genes, including Bcl-2 and MCL1, and the activation of ERK1/2 signaling. In addition, treatment of IL-17A to endometrial cells inhibited NK cell mediated cytotoxicity and induced HLA-G expression on endometrial cells. IL-17A also promoted migration of endometrial cells. Our data suggest that Th17 cells and IL-17A play critical roles in the development of endometriosis by promoting endometrial cell survival and conferring a resistance to NK cell cytotoxicity through the activation of ERK1/2 signaling. Targeting IL-17A has potential as a new strategy for the treatment of endometriosis. |
format | Online Article Text |
id | pubmed-10166657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Korean Association of Immunologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-101666572023-05-10 IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway Kang, Young-Ju Cho, Hee Jun Lee, Yunhee Park, Arum Kim, Mi Jeong Jeung, In Cheul Jung, Yong-Wook Jung, Haiyoung Choi, Inpyo Lee, Hee Gu Yoon, Suk Ran Immune Netw Original Article Immune status including the immune cells and cytokine profiles has been implicated in the development of endometriosis. In this study, we analyzed Th17 cells and IL-17A in peritoneal fluid (PF) and endometrial tissues of patients with (n=10) and without (n=26) endometriosis. Our study has shown increased Th17 cell population and IL-17A level in PF with endometriosis patients. To determine the roles of IL-17A and Th17 cells in the development of endometriosis, the effect of IL-17A, major cytokine of Th17, on endometrial cells isolated from endometriotic tissues was examined. Recombinant IL-17A promoted survival of endometrial cells accompanied by increased expression of anti-apoptotic genes, including Bcl-2 and MCL1, and the activation of ERK1/2 signaling. In addition, treatment of IL-17A to endometrial cells inhibited NK cell mediated cytotoxicity and induced HLA-G expression on endometrial cells. IL-17A also promoted migration of endometrial cells. Our data suggest that Th17 cells and IL-17A play critical roles in the development of endometriosis by promoting endometrial cell survival and conferring a resistance to NK cell cytotoxicity through the activation of ERK1/2 signaling. Targeting IL-17A has potential as a new strategy for the treatment of endometriosis. The Korean Association of Immunologists 2023-01-30 /pmc/articles/PMC10166657/ /pubmed/37179747 http://dx.doi.org/10.4110/in.2023.23.e14 Text en Copyright © 2023. The Korean Association of Immunologists https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kang, Young-Ju Cho, Hee Jun Lee, Yunhee Park, Arum Kim, Mi Jeong Jeung, In Cheul Jung, Yong-Wook Jung, Haiyoung Choi, Inpyo Lee, Hee Gu Yoon, Suk Ran IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway |
title | IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway |
title_full | IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway |
title_fullStr | IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway |
title_full_unstemmed | IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway |
title_short | IL-17A and Th17 Cells Contribute to Endometrial Cell Survival by Inhibiting Apoptosis and NK Cell Mediated Cytotoxicity of Endometrial Cells via ERK1/2 Pathway |
title_sort | il-17a and th17 cells contribute to endometrial cell survival by inhibiting apoptosis and nk cell mediated cytotoxicity of endometrial cells via erk1/2 pathway |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166657/ https://www.ncbi.nlm.nih.gov/pubmed/37179747 http://dx.doi.org/10.4110/in.2023.23.e14 |
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