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Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain
Pain is a persistent symptom of Rheumatoid Arthritis, and the K/BxN serum transfer model recapitulates both association and dissociation between pain and joint inflammation in RA. Furthermore, this model features monocyte/macrophage infiltration in joints and lumbar dorsal root ganglia (DRG), where...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166715/ https://www.ncbi.nlm.nih.gov/pubmed/36115544 http://dx.doi.org/10.1016/j.bbi.2022.09.008 |
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author | Oggero, Silvia Cecconello, Chiara Silva, Rita Zeboudj, Lynda Sideris-Lampretsas, George Perretti, Mauro Malcangio, Marzia |
author_facet | Oggero, Silvia Cecconello, Chiara Silva, Rita Zeboudj, Lynda Sideris-Lampretsas, George Perretti, Mauro Malcangio, Marzia |
author_sort | Oggero, Silvia |
collection | PubMed |
description | Pain is a persistent symptom of Rheumatoid Arthritis, and the K/BxN serum transfer model recapitulates both association and dissociation between pain and joint inflammation in RA. Furthermore, this model features monocyte/macrophage infiltration in joints and lumbar dorsal root ganglia (DRG), where these immune cells are close to nociceptive neurons. We focussed on CX(3)CR(1)-monocyte/macrophage trafficking and show that at peak paw swelling associated with nociception, CX(3)CR(1) deletion altered neither swelling nor macrophage infiltration/phenotype in paws. However, acute nociception and DRG non-classical monocyte numbers were reduced in CX(3)CR(1)(GFP/GFP) (KO) compared to CX(3)CR(1)(+/GFP) (WT). Nociception that persisted despite swelling had resolved was attenuated in KO and correlated with DRG macrophages displaying M2-like phenotype. Still in the DRG, neurons up-regulated neuropeptide CGRP and olcegepant treatment reduced acute swelling, nociception, and leukocyte infiltration in paws and DRG. We delineate in-vitro a signalling pathway showing that CGRP liberates the CX(3)CR(1) ligand fractalkine (FKN) from endothelium, and in bone marrow-derived macrophages, FKN promotes activation of intracellular kinases, polarisation towards M1-like phenotype and release of pro-nociceptive IL-6. These data implicate non-classical CX(3)CR(1)-expressing monocyte and macrophage recruitment into the DRG in initiation and maintenance of arthritis pain. |
format | Online Article Text |
id | pubmed-10166715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-101667152023-05-10 Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain Oggero, Silvia Cecconello, Chiara Silva, Rita Zeboudj, Lynda Sideris-Lampretsas, George Perretti, Mauro Malcangio, Marzia Brain Behav Immun Article Pain is a persistent symptom of Rheumatoid Arthritis, and the K/BxN serum transfer model recapitulates both association and dissociation between pain and joint inflammation in RA. Furthermore, this model features monocyte/macrophage infiltration in joints and lumbar dorsal root ganglia (DRG), where these immune cells are close to nociceptive neurons. We focussed on CX(3)CR(1)-monocyte/macrophage trafficking and show that at peak paw swelling associated with nociception, CX(3)CR(1) deletion altered neither swelling nor macrophage infiltration/phenotype in paws. However, acute nociception and DRG non-classical monocyte numbers were reduced in CX(3)CR(1)(GFP/GFP) (KO) compared to CX(3)CR(1)(+/GFP) (WT). Nociception that persisted despite swelling had resolved was attenuated in KO and correlated with DRG macrophages displaying M2-like phenotype. Still in the DRG, neurons up-regulated neuropeptide CGRP and olcegepant treatment reduced acute swelling, nociception, and leukocyte infiltration in paws and DRG. We delineate in-vitro a signalling pathway showing that CGRP liberates the CX(3)CR(1) ligand fractalkine (FKN) from endothelium, and in bone marrow-derived macrophages, FKN promotes activation of intracellular kinases, polarisation towards M1-like phenotype and release of pro-nociceptive IL-6. These data implicate non-classical CX(3)CR(1)-expressing monocyte and macrophage recruitment into the DRG in initiation and maintenance of arthritis pain. Elsevier 2022-11 /pmc/articles/PMC10166715/ /pubmed/36115544 http://dx.doi.org/10.1016/j.bbi.2022.09.008 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Oggero, Silvia Cecconello, Chiara Silva, Rita Zeboudj, Lynda Sideris-Lampretsas, George Perretti, Mauro Malcangio, Marzia Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain |
title | Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain |
title_full | Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain |
title_fullStr | Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain |
title_full_unstemmed | Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain |
title_short | Dorsal root ganglia CX3CR1 expressing monocytes/macrophages contribute to arthritis pain |
title_sort | dorsal root ganglia cx3cr1 expressing monocytes/macrophages contribute to arthritis pain |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10166715/ https://www.ncbi.nlm.nih.gov/pubmed/36115544 http://dx.doi.org/10.1016/j.bbi.2022.09.008 |
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