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Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub
Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacB...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167230/ https://www.ncbi.nlm.nih.gov/pubmed/37156769 http://dx.doi.org/10.1038/s41597-023-02171-6 |
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author | Zhao, Shi-Wei Guo, Jing-Fang Kong, Lei Nie, Shuai Yan, Xue-Mei Shi, Tian-Le Tian, Xue-Chan Ma, Hai-Yao Bao, Yu-Tao Li, Zhi-Chao Chen, Zhao-Yang Zhang, Ren-Gang Ma, Yong-Peng El-Kassaby, Yousry A. Porth, Ilga Zhao, Wei Mao, Jian-Feng |
author_facet | Zhao, Shi-Wei Guo, Jing-Fang Kong, Lei Nie, Shuai Yan, Xue-Mei Shi, Tian-Le Tian, Xue-Chan Ma, Hai-Yao Bao, Yu-Tao Li, Zhi-Chao Chen, Zhao-Yang Zhang, Ren-Gang Ma, Yong-Peng El-Kassaby, Yousry A. Porth, Ilga Zhao, Wei Mao, Jian-Feng |
author_sort | Zhao, Shi-Wei |
collection | PubMed |
description | Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis. |
format | Online Article Text |
id | pubmed-10167230 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101672302023-05-10 Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub Zhao, Shi-Wei Guo, Jing-Fang Kong, Lei Nie, Shuai Yan, Xue-Mei Shi, Tian-Le Tian, Xue-Chan Ma, Hai-Yao Bao, Yu-Tao Li, Zhi-Chao Chen, Zhao-Yang Zhang, Ren-Gang Ma, Yong-Peng El-Kassaby, Yousry A. Porth, Ilga Zhao, Wei Mao, Jian-Feng Sci Data Data Descriptor Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis. Nature Publishing Group UK 2023-05-09 /pmc/articles/PMC10167230/ /pubmed/37156769 http://dx.doi.org/10.1038/s41597-023-02171-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Data Descriptor Zhao, Shi-Wei Guo, Jing-Fang Kong, Lei Nie, Shuai Yan, Xue-Mei Shi, Tian-Le Tian, Xue-Chan Ma, Hai-Yao Bao, Yu-Tao Li, Zhi-Chao Chen, Zhao-Yang Zhang, Ren-Gang Ma, Yong-Peng El-Kassaby, Yousry A. Porth, Ilga Zhao, Wei Mao, Jian-Feng Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub |
title | Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub |
title_full | Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub |
title_fullStr | Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub |
title_full_unstemmed | Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub |
title_short | Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub |
title_sort | haplotype-resolved genome assembly of coriaria nepalensis a non-legume nitrogen-fixing shrub |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167230/ https://www.ncbi.nlm.nih.gov/pubmed/37156769 http://dx.doi.org/10.1038/s41597-023-02171-6 |
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