Cargando…

A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea

In recent years, new emerging pathogenic microorganisms have frequently appeared in animals, including marine fish, possibly due to climate change, anthropogenic activities, and even cross-species transmission of pathogenic microorganisms among animals or between animals and humans, which poses a se...

Descripción completa

Detalles Bibliográficos
Autores principales: Meng, Xiangyu, Chen, Fangyi, Xiong, Ming, Hao, Hua, Wang, Ke-Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167289/
https://www.ncbi.nlm.nih.gov/pubmed/37180261
http://dx.doi.org/10.3389/fmicb.2023.1129568
_version_ 1785038631322255360
author Meng, Xiangyu
Chen, Fangyi
Xiong, Ming
Hao, Hua
Wang, Ke-Jian
author_facet Meng, Xiangyu
Chen, Fangyi
Xiong, Ming
Hao, Hua
Wang, Ke-Jian
author_sort Meng, Xiangyu
collection PubMed
description In recent years, new emerging pathogenic microorganisms have frequently appeared in animals, including marine fish, possibly due to climate change, anthropogenic activities, and even cross-species transmission of pathogenic microorganisms among animals or between animals and humans, which poses a serious issue for preventive medicine. In this study, a bacterium was clearly characterized among 64 isolates from the gills of diseased large yellow croaker Larimichthys crocea that were raised in marine aquaculture. This strain was identified as K. kristinae by biochemical tests with a VITEK 2.0 analysis system and 16S rRNA sequencing and named K. kristinae_LC. The potential genes that might encode virulence-factors were widely screened through sequence analysis of the whole genome of K. kristinae_LC. Many genes involved in the two-component system and drug-resistance were also annotated. In addition, 104 unique genes in K. kristinae_LC were identified by pan genome analysis with the genomes of this strain from five different origins (woodpecker, medical resource, environment, and marine sponge reef) and the analysis results demonstrated that their predicted functions might be associated with adaptation to living conditions such as higher salinity, complex marine biomes, and low temperature. A significant difference in genomic organization was found among the K. kristinae strains that might be related to their hosts living in different environments. The animal regression test for this new bacterial isolate was carried out using L. crocea, and the results showed that this bacterium could cause the death of L. crocea and that the fish mortality was dose-dependent within 5 days post infection, indicating the pathogenicity of K. kristinae_LC to marine fish. Since K. kristinae has been reported as a pathogen for humans and bovines, in our study, we revealed a new isolate of K. kristinae_LC from marine fish for the first time, suggesting the potentiality of cross-species transmission among animals or from marine animals to humans, from which we would gain insight to help in future public prevention strategies for new emerging pathogens.
format Online
Article
Text
id pubmed-10167289
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-101672892023-05-10 A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea Meng, Xiangyu Chen, Fangyi Xiong, Ming Hao, Hua Wang, Ke-Jian Front Microbiol Microbiology In recent years, new emerging pathogenic microorganisms have frequently appeared in animals, including marine fish, possibly due to climate change, anthropogenic activities, and even cross-species transmission of pathogenic microorganisms among animals or between animals and humans, which poses a serious issue for preventive medicine. In this study, a bacterium was clearly characterized among 64 isolates from the gills of diseased large yellow croaker Larimichthys crocea that were raised in marine aquaculture. This strain was identified as K. kristinae by biochemical tests with a VITEK 2.0 analysis system and 16S rRNA sequencing and named K. kristinae_LC. The potential genes that might encode virulence-factors were widely screened through sequence analysis of the whole genome of K. kristinae_LC. Many genes involved in the two-component system and drug-resistance were also annotated. In addition, 104 unique genes in K. kristinae_LC were identified by pan genome analysis with the genomes of this strain from five different origins (woodpecker, medical resource, environment, and marine sponge reef) and the analysis results demonstrated that their predicted functions might be associated with adaptation to living conditions such as higher salinity, complex marine biomes, and low temperature. A significant difference in genomic organization was found among the K. kristinae strains that might be related to their hosts living in different environments. The animal regression test for this new bacterial isolate was carried out using L. crocea, and the results showed that this bacterium could cause the death of L. crocea and that the fish mortality was dose-dependent within 5 days post infection, indicating the pathogenicity of K. kristinae_LC to marine fish. Since K. kristinae has been reported as a pathogen for humans and bovines, in our study, we revealed a new isolate of K. kristinae_LC from marine fish for the first time, suggesting the potentiality of cross-species transmission among animals or from marine animals to humans, from which we would gain insight to help in future public prevention strategies for new emerging pathogens. Frontiers Media S.A. 2023-04-25 /pmc/articles/PMC10167289/ /pubmed/37180261 http://dx.doi.org/10.3389/fmicb.2023.1129568 Text en Copyright © 2023 Meng, Chen, Xiong, Hao and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Meng, Xiangyu
Chen, Fangyi
Xiong, Ming
Hao, Hua
Wang, Ke-Jian
A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea
title A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea
title_full A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea
title_fullStr A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea
title_full_unstemmed A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea
title_short A new pathogenic isolate of Kocuria kristinae identified for the first time in the marine fish Larimichthys crocea
title_sort new pathogenic isolate of kocuria kristinae identified for the first time in the marine fish larimichthys crocea
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167289/
https://www.ncbi.nlm.nih.gov/pubmed/37180261
http://dx.doi.org/10.3389/fmicb.2023.1129568
work_keys_str_mv AT mengxiangyu anewpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT chenfangyi anewpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT xiongming anewpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT haohua anewpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT wangkejian anewpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT mengxiangyu newpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT chenfangyi newpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT xiongming newpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT haohua newpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea
AT wangkejian newpathogenicisolateofkocuriakristinaeidentifiedforthefirsttimeinthemarinefishlarimichthyscrocea