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Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A. hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus
Recently, nanoparticles have attracted attention as a preventive tool for certain infectious diseases affecting fish in aquaculture. Furthermore, freshwater fishes are frequently vulnerable to summer mass morality caused by Aeromonas bacteria. In this regard, we focused on the evaluation of the in v...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167877/ https://www.ncbi.nlm.nih.gov/pubmed/37179529 http://dx.doi.org/10.1080/23144599.2023.2205338 |
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author | Aly, Salah M. Eissa, Alaa Eldin Abdel-Razek, Nashwa El-Ramlawy, Asmaa O. |
author_facet | Aly, Salah M. Eissa, Alaa Eldin Abdel-Razek, Nashwa El-Ramlawy, Asmaa O. |
author_sort | Aly, Salah M. |
collection | PubMed |
description | Recently, nanoparticles have attracted attention as a preventive tool for certain infectious diseases affecting fish in aquaculture. Furthermore, freshwater fishes are frequently vulnerable to summer mass morality caused by Aeromonas bacteria. In this regard, we focused on the evaluation of the in vitro and in vivo antimicrobial activity of chitosan (CNPs) and silver (AgNPs) nanoparticles against Aeromonas hydrophila subsp. hydrophila. CNPs and AgNPs were prepared at a mean particle size of 9.03 and 12.8 nm and a charge equalled+36.4 and −19.3 mV for CNPs and AgNPs, respectively. A. hydrophila subsp. hydrophila, Aeromonas caviae, and Aeromonas punctata were retrieved and identified by traditional and molecular techniques. The sensitivity of the obtained bacteria to eight different antibiotic discs was also tested. The antibiotic sensitivity studies revealed the presence of multidrug-resistant (MDR) Aeromonas species (spp.). The bacterium that showed the highest multidrug resistance against the tested antibiotic discs was Aeromonas hydrophila subsp. hydrophila. Therefore, CNPs and AgNPs were in vitro tested against the isolated bacterium and exhibited inhibition zones of 15 and 25 mm, respectively. TEM images also showed that CNPs and AgNPs had an antagonistic action against the same bacterium causing loss of architecture and bacterial death. |
format | Online Article Text |
id | pubmed-10167877 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-101678772023-05-10 Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A. hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus Aly, Salah M. Eissa, Alaa Eldin Abdel-Razek, Nashwa El-Ramlawy, Asmaa O. Int J Vet Sci Med Research Article Recently, nanoparticles have attracted attention as a preventive tool for certain infectious diseases affecting fish in aquaculture. Furthermore, freshwater fishes are frequently vulnerable to summer mass morality caused by Aeromonas bacteria. In this regard, we focused on the evaluation of the in vitro and in vivo antimicrobial activity of chitosan (CNPs) and silver (AgNPs) nanoparticles against Aeromonas hydrophila subsp. hydrophila. CNPs and AgNPs were prepared at a mean particle size of 9.03 and 12.8 nm and a charge equalled+36.4 and −19.3 mV for CNPs and AgNPs, respectively. A. hydrophila subsp. hydrophila, Aeromonas caviae, and Aeromonas punctata were retrieved and identified by traditional and molecular techniques. The sensitivity of the obtained bacteria to eight different antibiotic discs was also tested. The antibiotic sensitivity studies revealed the presence of multidrug-resistant (MDR) Aeromonas species (spp.). The bacterium that showed the highest multidrug resistance against the tested antibiotic discs was Aeromonas hydrophila subsp. hydrophila. Therefore, CNPs and AgNPs were in vitro tested against the isolated bacterium and exhibited inhibition zones of 15 and 25 mm, respectively. TEM images also showed that CNPs and AgNPs had an antagonistic action against the same bacterium causing loss of architecture and bacterial death. Taylor & Francis 2023-05-08 /pmc/articles/PMC10167877/ /pubmed/37179529 http://dx.doi.org/10.1080/23144599.2023.2205338 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent. |
spellingShingle | Research Article Aly, Salah M. Eissa, Alaa Eldin Abdel-Razek, Nashwa El-Ramlawy, Asmaa O. Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A. hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus |
title | Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A.
hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus |
title_full | Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A.
hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus |
title_fullStr | Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A.
hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus |
title_full_unstemmed | Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A.
hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus |
title_short | Chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing A.
hydrophila subsp. hydrophila infection in Nile tilapia, Oreochromis niloticus |
title_sort | chitosan nanoparticles and green synthesized silver nanoparticles as novel alternatives to antibiotics for preventing a.
hydrophila subsp. hydrophila infection in nile tilapia, oreochromis niloticus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10167877/ https://www.ncbi.nlm.nih.gov/pubmed/37179529 http://dx.doi.org/10.1080/23144599.2023.2205338 |
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