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Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation
OBJECTIVES: Peptidoglycan (PG) is an arthritogenic bacterial cell wall component whose role in human osteoarthritis is poorly understood. The purpose of this study was to determine if PG is present in synovial tissue of osteoarthritis patients at the time of primary total knee arthroplasty (TKA), an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Journal Experts
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10168439/ https://www.ncbi.nlm.nih.gov/pubmed/37162851 http://dx.doi.org/10.21203/rs.3.rs-2842385/v1 |
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author | Holub, Meaghan N Wahhab, Amanda Rouse, Joseph R Danner, Rebecca McClune, Mecaila M Dressler, Jules M Strle, Klemen Jutras, Brandon L Edelstein, Adam I Lochhead, Robert B |
author_facet | Holub, Meaghan N Wahhab, Amanda Rouse, Joseph R Danner, Rebecca McClune, Mecaila M Dressler, Jules M Strle, Klemen Jutras, Brandon L Edelstein, Adam I Lochhead, Robert B |
author_sort | Holub, Meaghan N |
collection | PubMed |
description | OBJECTIVES: Peptidoglycan (PG) is an arthritogenic bacterial cell wall component whose role in human osteoarthritis is poorly understood. The purpose of this study was to determine if PG is present in synovial tissue of osteoarthritis patients at the time of primary total knee arthroplasty (TKA), and if its presence is associated with inflammation and patient reported outcomes. METHODS: Intraoperative synovial tissue and synovial fluid samples were obtained from 56 patients undergoing primary TKA, none of whom had history of infection. PG in synovial tissue was detected by immunohistochemistry (IHC). Synovial tissue inflammation and fibrosis were assessed by histopathology and synovial fluid cytokine quantification. Primary human fibroblasts isolated from arthritis synovial tissue were stimulated with PG to determine inflammatory cytokine response. RESULTS: A total of 33/56 (59%) of primary TKA synovial tissue samples were positive for PG by IHC, with mean 8 PG occurrences per 10 mm(2) of tissue in PG-positive samples. Synovial tissue inflammation and elevated IL-6 in synovial fluid positively correlated with PG positivity. Primary human fibroblasts stimulated with PG secreted high levels of IL-6, consistent with ex vivo findings. Interestingly, we observed a significant inverse correlation between PG and age at time of TKA, indicating younger age at time of TKA was associated with higher PG levels. CONCLUSION: Peptidoglycan is commonly found in synovial tissue from patients undergoing TKA. Our data indicate that PG may play an important role in inflammatory synovitis, particularly in patients who undergo TKA at a relatively younger age. |
format | Online Article Text |
id | pubmed-10168439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Journal Experts |
record_format | MEDLINE/PubMed |
spelling | pubmed-101684392023-05-10 Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation Holub, Meaghan N Wahhab, Amanda Rouse, Joseph R Danner, Rebecca McClune, Mecaila M Dressler, Jules M Strle, Klemen Jutras, Brandon L Edelstein, Adam I Lochhead, Robert B Res Sq Article OBJECTIVES: Peptidoglycan (PG) is an arthritogenic bacterial cell wall component whose role in human osteoarthritis is poorly understood. The purpose of this study was to determine if PG is present in synovial tissue of osteoarthritis patients at the time of primary total knee arthroplasty (TKA), and if its presence is associated with inflammation and patient reported outcomes. METHODS: Intraoperative synovial tissue and synovial fluid samples were obtained from 56 patients undergoing primary TKA, none of whom had history of infection. PG in synovial tissue was detected by immunohistochemistry (IHC). Synovial tissue inflammation and fibrosis were assessed by histopathology and synovial fluid cytokine quantification. Primary human fibroblasts isolated from arthritis synovial tissue were stimulated with PG to determine inflammatory cytokine response. RESULTS: A total of 33/56 (59%) of primary TKA synovial tissue samples were positive for PG by IHC, with mean 8 PG occurrences per 10 mm(2) of tissue in PG-positive samples. Synovial tissue inflammation and elevated IL-6 in synovial fluid positively correlated with PG positivity. Primary human fibroblasts stimulated with PG secreted high levels of IL-6, consistent with ex vivo findings. Interestingly, we observed a significant inverse correlation between PG and age at time of TKA, indicating younger age at time of TKA was associated with higher PG levels. CONCLUSION: Peptidoglycan is commonly found in synovial tissue from patients undergoing TKA. Our data indicate that PG may play an important role in inflammatory synovitis, particularly in patients who undergo TKA at a relatively younger age. American Journal Experts 2023-04-28 /pmc/articles/PMC10168439/ /pubmed/37162851 http://dx.doi.org/10.21203/rs.3.rs-2842385/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use. |
spellingShingle | Article Holub, Meaghan N Wahhab, Amanda Rouse, Joseph R Danner, Rebecca McClune, Mecaila M Dressler, Jules M Strle, Klemen Jutras, Brandon L Edelstein, Adam I Lochhead, Robert B Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation |
title | Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation |
title_full | Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation |
title_fullStr | Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation |
title_full_unstemmed | Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation |
title_short | Peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation |
title_sort | peptidoglycan in osteoarthritis synovial tissue is associated with joint inflammation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10168439/ https://www.ncbi.nlm.nih.gov/pubmed/37162851 http://dx.doi.org/10.21203/rs.3.rs-2842385/v1 |
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