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A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria

The necrotrophic plant pathogenic bacterium Dickeya solani emerged in the potato agrosystem in Europe. All isolated strains of D. solani contain several large polyketide synthase/non-ribosomal peptide synthetase (PKS/NRPS) gene clusters. Analogy with genes described in other bacteria suggests that t...

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Autores principales: Brual, Typhaine, Effantin, Géraldine, Baltenneck, Julie, Attaiech, Laetitia, Grosbois, Cloé, Royer, Monique, Cigna, Jérémy, Faure, Denis, Hugouvieux-Cotte-Pattat, Nicole, Gueguen, Erwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10168573/
https://www.ncbi.nlm.nih.gov/pubmed/37104544
http://dx.doi.org/10.1371/journal.pgen.1010725
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author Brual, Typhaine
Effantin, Géraldine
Baltenneck, Julie
Attaiech, Laetitia
Grosbois, Cloé
Royer, Monique
Cigna, Jérémy
Faure, Denis
Hugouvieux-Cotte-Pattat, Nicole
Gueguen, Erwan
author_facet Brual, Typhaine
Effantin, Géraldine
Baltenneck, Julie
Attaiech, Laetitia
Grosbois, Cloé
Royer, Monique
Cigna, Jérémy
Faure, Denis
Hugouvieux-Cotte-Pattat, Nicole
Gueguen, Erwan
author_sort Brual, Typhaine
collection PubMed
description The necrotrophic plant pathogenic bacterium Dickeya solani emerged in the potato agrosystem in Europe. All isolated strains of D. solani contain several large polyketide synthase/non-ribosomal peptide synthetase (PKS/NRPS) gene clusters. Analogy with genes described in other bacteria suggests that the clusters ooc and zms are involved in the production of secondary metabolites of the oocydin and zeamine families, respectively. A third cluster named sol was recently shown to produce an antifungal molecule. In this study, we constructed mutants impaired in each of the three secondary metabolite clusters sol, ooc, and zms to compare first the phenotype of the D. solani wild-type strain D s0432-1 with its associated mutants. We demonstrated the antimicrobial functions of these three PKS/NRPS clusters against bacteria, yeasts or fungi. The cluster sol, conserved in several other Dickeya species, produces a secondary metabolite inhibiting yeasts. Phenotyping and comparative genomics of different D. solani wild-type isolates revealed that the small regulatory RNA ArcZ plays a major role in the control of the clusters sol and zms. A single-point mutation, conserved in some Dickeya wild-type strains, including the D. solani type strain IPO 2222, impairs the ArcZ function by affecting its processing into an active form.
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spelling pubmed-101685732023-05-10 A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria Brual, Typhaine Effantin, Géraldine Baltenneck, Julie Attaiech, Laetitia Grosbois, Cloé Royer, Monique Cigna, Jérémy Faure, Denis Hugouvieux-Cotte-Pattat, Nicole Gueguen, Erwan PLoS Genet Research Article The necrotrophic plant pathogenic bacterium Dickeya solani emerged in the potato agrosystem in Europe. All isolated strains of D. solani contain several large polyketide synthase/non-ribosomal peptide synthetase (PKS/NRPS) gene clusters. Analogy with genes described in other bacteria suggests that the clusters ooc and zms are involved in the production of secondary metabolites of the oocydin and zeamine families, respectively. A third cluster named sol was recently shown to produce an antifungal molecule. In this study, we constructed mutants impaired in each of the three secondary metabolite clusters sol, ooc, and zms to compare first the phenotype of the D. solani wild-type strain D s0432-1 with its associated mutants. We demonstrated the antimicrobial functions of these three PKS/NRPS clusters against bacteria, yeasts or fungi. The cluster sol, conserved in several other Dickeya species, produces a secondary metabolite inhibiting yeasts. Phenotyping and comparative genomics of different D. solani wild-type isolates revealed that the small regulatory RNA ArcZ plays a major role in the control of the clusters sol and zms. A single-point mutation, conserved in some Dickeya wild-type strains, including the D. solani type strain IPO 2222, impairs the ArcZ function by affecting its processing into an active form. Public Library of Science 2023-04-27 /pmc/articles/PMC10168573/ /pubmed/37104544 http://dx.doi.org/10.1371/journal.pgen.1010725 Text en © 2023 Brual et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Brual, Typhaine
Effantin, Géraldine
Baltenneck, Julie
Attaiech, Laetitia
Grosbois, Cloé
Royer, Monique
Cigna, Jérémy
Faure, Denis
Hugouvieux-Cotte-Pattat, Nicole
Gueguen, Erwan
A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria
title A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria
title_full A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria
title_fullStr A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria
title_full_unstemmed A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria
title_short A natural single nucleotide mutation in the small regulatory RNA ArcZ of Dickeya solani switches off the antimicrobial activities against yeast and bacteria
title_sort natural single nucleotide mutation in the small regulatory rna arcz of dickeya solani switches off the antimicrobial activities against yeast and bacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10168573/
https://www.ncbi.nlm.nih.gov/pubmed/37104544
http://dx.doi.org/10.1371/journal.pgen.1010725
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