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Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress

Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influenc...

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Autores principales: Abu Quba, Abd Alaziz, Goebel, Marc-Oliver, Karagulyan, Mariam, Miltner, Anja, Kästner, Matthias, Bachmann, Jörg, Schaumann, Gabriele E, Diehl, Doerte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169395/
https://www.ncbi.nlm.nih.gov/pubmed/37333443
http://dx.doi.org/10.1093/femsmc/xtac028
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author Abu Quba, Abd Alaziz
Goebel, Marc-Oliver
Karagulyan, Mariam
Miltner, Anja
Kästner, Matthias
Bachmann, Jörg
Schaumann, Gabriele E
Diehl, Doerte
author_facet Abu Quba, Abd Alaziz
Goebel, Marc-Oliver
Karagulyan, Mariam
Miltner, Anja
Kästner, Matthias
Bachmann, Jörg
Schaumann, Gabriele E
Diehl, Doerte
author_sort Abu Quba, Abd Alaziz
collection PubMed
description Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area.
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spelling pubmed-101693952023-06-16 Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress Abu Quba, Abd Alaziz Goebel, Marc-Oliver Karagulyan, Mariam Miltner, Anja Kästner, Matthias Bachmann, Jörg Schaumann, Gabriele E Diehl, Doerte FEMS Microbes Research Article Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area. Oxford University Press 2022-12-10 /pmc/articles/PMC10169395/ /pubmed/37333443 http://dx.doi.org/10.1093/femsmc/xtac028 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Article
Abu Quba, Abd Alaziz
Goebel, Marc-Oliver
Karagulyan, Mariam
Miltner, Anja
Kästner, Matthias
Bachmann, Jörg
Schaumann, Gabriele E
Diehl, Doerte
Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
title Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
title_full Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
title_fullStr Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
title_full_unstemmed Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
title_short Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
title_sort changes in cell surface properties of pseudomonas fluorescens by adaptation to nacl induced hypertonic stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169395/
https://www.ncbi.nlm.nih.gov/pubmed/37333443
http://dx.doi.org/10.1093/femsmc/xtac028
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