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Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress
Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influenc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169395/ https://www.ncbi.nlm.nih.gov/pubmed/37333443 http://dx.doi.org/10.1093/femsmc/xtac028 |
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author | Abu Quba, Abd Alaziz Goebel, Marc-Oliver Karagulyan, Mariam Miltner, Anja Kästner, Matthias Bachmann, Jörg Schaumann, Gabriele E Diehl, Doerte |
author_facet | Abu Quba, Abd Alaziz Goebel, Marc-Oliver Karagulyan, Mariam Miltner, Anja Kästner, Matthias Bachmann, Jörg Schaumann, Gabriele E Diehl, Doerte |
author_sort | Abu Quba, Abd Alaziz |
collection | PubMed |
description | Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area. |
format | Online Article Text |
id | pubmed-10169395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-101693952023-06-16 Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress Abu Quba, Abd Alaziz Goebel, Marc-Oliver Karagulyan, Mariam Miltner, Anja Kästner, Matthias Bachmann, Jörg Schaumann, Gabriele E Diehl, Doerte FEMS Microbes Research Article Determination of the effect of water stress on the surface properties of bacteria is crucial to study bacterial induced soil water repellency. Changes in the environmental conditions may affect several properties of bacteria such as the cell hydrophobicity and morphology. Here, we study the influence of adaptation to hypertonic stress on cell wettability, shape, adhesion, and surface chemical composition of Pseudomonas fluorescens. From this we aim to discover possible relations between the changes in wettability of bacterial films studied by contact angle and single cells studied by atomic and chemical force microscopy (AFM, CFM), which is still lacking. We show that by stress the adhesion forces of the cell surfaces towards hydrophobic functionalized probes increase while they decrease towards hydrophilic functionalized tips. This is consistent with the contact angle results. Further, cell size shrunk and protein content increased upon stress. The results suggest two possible mechanisms: Cell shrinkage is accompanied by the release of outer membrane vesicles by which the protein to lipid ratio increases. The higher protein content increases the rigidity and the number of hydrophobic nano-domains per surface area. Oxford University Press 2022-12-10 /pmc/articles/PMC10169395/ /pubmed/37333443 http://dx.doi.org/10.1093/femsmc/xtac028 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of FEMS. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Research Article Abu Quba, Abd Alaziz Goebel, Marc-Oliver Karagulyan, Mariam Miltner, Anja Kästner, Matthias Bachmann, Jörg Schaumann, Gabriele E Diehl, Doerte Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress |
title | Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress |
title_full | Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress |
title_fullStr | Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress |
title_full_unstemmed | Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress |
title_short | Changes in cell surface properties of Pseudomonas fluorescens by adaptation to NaCl induced hypertonic stress |
title_sort | changes in cell surface properties of pseudomonas fluorescens by adaptation to nacl induced hypertonic stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169395/ https://www.ncbi.nlm.nih.gov/pubmed/37333443 http://dx.doi.org/10.1093/femsmc/xtac028 |
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