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Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation

BACKGROUND: Phthalates (PAEs) are important synthetic substances in plastics, attracting much attention due to their potential effects on the cardiovascular system. METHODS: In this study, urine and blood samples from 39 individuals were collected in Tianjin, China. Phthalates and phthalate metaboli...

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Autores principales: Li, Weixia, Guo, Liqiong, Fang, Junkai, Zhao, Lei, Song, Shanjun, Fang, Tao, Li, Chenguang, Wang, Lei, Li, Penghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169620/
https://www.ncbi.nlm.nih.gov/pubmed/37181721
http://dx.doi.org/10.3389/fpubh.2023.1108555
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author Li, Weixia
Guo, Liqiong
Fang, Junkai
Zhao, Lei
Song, Shanjun
Fang, Tao
Li, Chenguang
Wang, Lei
Li, Penghui
author_facet Li, Weixia
Guo, Liqiong
Fang, Junkai
Zhao, Lei
Song, Shanjun
Fang, Tao
Li, Chenguang
Wang, Lei
Li, Penghui
author_sort Li, Weixia
collection PubMed
description BACKGROUND: Phthalates (PAEs) are important synthetic substances in plastics, attracting much attention due to their potential effects on the cardiovascular system. METHODS: In this study, urine and blood samples from 39 individuals were collected in Tianjin, China. Phthalates and phthalate metabolites (mPAEs) were analyzed using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-mass spectrometry (HPLC-MS), respectively. The polymerase chain reaction (PCR) products from bisulfite-treated mitochondrial DNA (mtDNA) samples were analyzed using pyrosequencing technology. RESULTS: The detection frequencies for 9 PAEs varied from 2.56 to 92.31%, and those for 10 mPAEs varied from 30.77 to 100%. The estimated daily intakes (EDIs) and cumulative risk of PAEs were calculated based on the experimental statistics of urinary PAEs and mPAEs. For PAEs, the HI(RfD) (hazard index corresponding to reference doses) values of 10.26% of participants and the HI(TDI) (hazard index corresponding to tolerable daily intake) values of 30.77% of participants were estimated to exceed 1, suggesting a relatively high exposure risk. The mtDNA methylation levels in the MT-ATP8 and MT-ND5 were observed to be lower than in the MT-ATP6. Mono-ethyl phthalate (MEP) and MT-ATP8 were positively correlated with triglyceride levels (p < 0.05). Based on the association of PAEs, mtDNA methylation, and triglycerides, the mediating role of mtDNA methylation between PAEs and cardiovascular diseases (CVDs) was analyzed in this study, but no mediated effect was observed. CONCLUSION: The effects of PAE exposure on cardiovascular diseases (CVDs) should be investigated further.
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spelling pubmed-101696202023-05-11 Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation Li, Weixia Guo, Liqiong Fang, Junkai Zhao, Lei Song, Shanjun Fang, Tao Li, Chenguang Wang, Lei Li, Penghui Front Public Health Public Health BACKGROUND: Phthalates (PAEs) are important synthetic substances in plastics, attracting much attention due to their potential effects on the cardiovascular system. METHODS: In this study, urine and blood samples from 39 individuals were collected in Tianjin, China. Phthalates and phthalate metabolites (mPAEs) were analyzed using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-mass spectrometry (HPLC-MS), respectively. The polymerase chain reaction (PCR) products from bisulfite-treated mitochondrial DNA (mtDNA) samples were analyzed using pyrosequencing technology. RESULTS: The detection frequencies for 9 PAEs varied from 2.56 to 92.31%, and those for 10 mPAEs varied from 30.77 to 100%. The estimated daily intakes (EDIs) and cumulative risk of PAEs were calculated based on the experimental statistics of urinary PAEs and mPAEs. For PAEs, the HI(RfD) (hazard index corresponding to reference doses) values of 10.26% of participants and the HI(TDI) (hazard index corresponding to tolerable daily intake) values of 30.77% of participants were estimated to exceed 1, suggesting a relatively high exposure risk. The mtDNA methylation levels in the MT-ATP8 and MT-ND5 were observed to be lower than in the MT-ATP6. Mono-ethyl phthalate (MEP) and MT-ATP8 were positively correlated with triglyceride levels (p < 0.05). Based on the association of PAEs, mtDNA methylation, and triglycerides, the mediating role of mtDNA methylation between PAEs and cardiovascular diseases (CVDs) was analyzed in this study, but no mediated effect was observed. CONCLUSION: The effects of PAE exposure on cardiovascular diseases (CVDs) should be investigated further. Frontiers Media S.A. 2023-04-26 /pmc/articles/PMC10169620/ /pubmed/37181721 http://dx.doi.org/10.3389/fpubh.2023.1108555 Text en Copyright © 2023 Li, Guo, Fang, Zhao, Song, Fang, Li, Wang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Li, Weixia
Guo, Liqiong
Fang, Junkai
Zhao, Lei
Song, Shanjun
Fang, Tao
Li, Chenguang
Wang, Lei
Li, Penghui
Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation
title Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation
title_full Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation
title_fullStr Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation
title_full_unstemmed Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation
title_short Phthalates and phthalate metabolites in urine from Tianjin and implications for platelet mitochondrial DNA methylation
title_sort phthalates and phthalate metabolites in urine from tianjin and implications for platelet mitochondrial dna methylation
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169620/
https://www.ncbi.nlm.nih.gov/pubmed/37181721
http://dx.doi.org/10.3389/fpubh.2023.1108555
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