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Light-sheets and smart microscopy, an exciting future is dawning

Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capa...

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Autores principales: Daetwyler, Stephan, Fiolka, Reto Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169780/
https://www.ncbi.nlm.nih.gov/pubmed/37161000
http://dx.doi.org/10.1038/s42003-023-04857-4
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author Daetwyler, Stephan
Fiolka, Reto Paul
author_facet Daetwyler, Stephan
Fiolka, Reto Paul
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collection PubMed
description Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capabilities. This includes smart and adaptive imaging schemes to overcome traditional imaging trade-offs, i.e., spatiotemporal resolution, field of view and sample health. In smart microscopy, a microscope will autonomously decide where, when, what and how to image. We further assess how image restoration techniques provide avenues to overcome these tradeoffs and how “open top” light-sheet microscopes may enable multi-modal imaging with high throughput. As such, we predict that light-sheet microscopy will fulfill an important role in biomedical and clinical imaging in the future.
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spelling pubmed-101697802023-05-11 Light-sheets and smart microscopy, an exciting future is dawning Daetwyler, Stephan Fiolka, Reto Paul Commun Biol Review Article Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capabilities. This includes smart and adaptive imaging schemes to overcome traditional imaging trade-offs, i.e., spatiotemporal resolution, field of view and sample health. In smart microscopy, a microscope will autonomously decide where, when, what and how to image. We further assess how image restoration techniques provide avenues to overcome these tradeoffs and how “open top” light-sheet microscopes may enable multi-modal imaging with high throughput. As such, we predict that light-sheet microscopy will fulfill an important role in biomedical and clinical imaging in the future. Nature Publishing Group UK 2023-05-09 /pmc/articles/PMC10169780/ /pubmed/37161000 http://dx.doi.org/10.1038/s42003-023-04857-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review Article
Daetwyler, Stephan
Fiolka, Reto Paul
Light-sheets and smart microscopy, an exciting future is dawning
title Light-sheets and smart microscopy, an exciting future is dawning
title_full Light-sheets and smart microscopy, an exciting future is dawning
title_fullStr Light-sheets and smart microscopy, an exciting future is dawning
title_full_unstemmed Light-sheets and smart microscopy, an exciting future is dawning
title_short Light-sheets and smart microscopy, an exciting future is dawning
title_sort light-sheets and smart microscopy, an exciting future is dawning
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169780/
https://www.ncbi.nlm.nih.gov/pubmed/37161000
http://dx.doi.org/10.1038/s42003-023-04857-4
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