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Light-sheets and smart microscopy, an exciting future is dawning
Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capa...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169780/ https://www.ncbi.nlm.nih.gov/pubmed/37161000 http://dx.doi.org/10.1038/s42003-023-04857-4 |
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author | Daetwyler, Stephan Fiolka, Reto Paul |
author_facet | Daetwyler, Stephan Fiolka, Reto Paul |
author_sort | Daetwyler, Stephan |
collection | PubMed |
description | Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capabilities. This includes smart and adaptive imaging schemes to overcome traditional imaging trade-offs, i.e., spatiotemporal resolution, field of view and sample health. In smart microscopy, a microscope will autonomously decide where, when, what and how to image. We further assess how image restoration techniques provide avenues to overcome these tradeoffs and how “open top” light-sheet microscopes may enable multi-modal imaging with high throughput. As such, we predict that light-sheet microscopy will fulfill an important role in biomedical and clinical imaging in the future. |
format | Online Article Text |
id | pubmed-10169780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101697802023-05-11 Light-sheets and smart microscopy, an exciting future is dawning Daetwyler, Stephan Fiolka, Reto Paul Commun Biol Review Article Light-sheet fluorescence microscopy has transformed our ability to visualize and quantitatively measure biological processes rapidly and over long time periods. In this review, we discuss current and future developments in light-sheet fluorescence microscopy that we expect to further expand its capabilities. This includes smart and adaptive imaging schemes to overcome traditional imaging trade-offs, i.e., spatiotemporal resolution, field of view and sample health. In smart microscopy, a microscope will autonomously decide where, when, what and how to image. We further assess how image restoration techniques provide avenues to overcome these tradeoffs and how “open top” light-sheet microscopes may enable multi-modal imaging with high throughput. As such, we predict that light-sheet microscopy will fulfill an important role in biomedical and clinical imaging in the future. Nature Publishing Group UK 2023-05-09 /pmc/articles/PMC10169780/ /pubmed/37161000 http://dx.doi.org/10.1038/s42003-023-04857-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Review Article Daetwyler, Stephan Fiolka, Reto Paul Light-sheets and smart microscopy, an exciting future is dawning |
title | Light-sheets and smart microscopy, an exciting future is dawning |
title_full | Light-sheets and smart microscopy, an exciting future is dawning |
title_fullStr | Light-sheets and smart microscopy, an exciting future is dawning |
title_full_unstemmed | Light-sheets and smart microscopy, an exciting future is dawning |
title_short | Light-sheets and smart microscopy, an exciting future is dawning |
title_sort | light-sheets and smart microscopy, an exciting future is dawning |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10169780/ https://www.ncbi.nlm.nih.gov/pubmed/37161000 http://dx.doi.org/10.1038/s42003-023-04857-4 |
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