Cargando…
Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
L-DOPA is an amino acid that is used as a treatment for Parkinson’s disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme,...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japan Academy
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170061/ https://www.ncbi.nlm.nih.gov/pubmed/36908174 http://dx.doi.org/10.2183/pjab.99.006 |
Sumario: | L-DOPA is an amino acid that is used as a treatment for Parkinson’s disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression. |
---|