Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase

L-DOPA is an amino acid that is used as a treatment for Parkinson’s disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme,...

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Autores principales: KUMAGAI, Hidehiko, KATAYAMA, Takane, KOYANAGI, Takashi, SUZUKI, Hideyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japan Academy 2023
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170061/
https://www.ncbi.nlm.nih.gov/pubmed/36908174
http://dx.doi.org/10.2183/pjab.99.006
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author KUMAGAI, Hidehiko
KATAYAMA, Takane
KOYANAGI, Takashi
SUZUKI, Hideyuki
author_facet KUMAGAI, Hidehiko
KATAYAMA, Takane
KOYANAGI, Takashi
SUZUKI, Hideyuki
author_sort KUMAGAI, Hidehiko
collection PubMed
description L-DOPA is an amino acid that is used as a treatment for Parkinson’s disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression.
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spelling pubmed-101700612023-05-11 Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase KUMAGAI, Hidehiko KATAYAMA, Takane KOYANAGI, Takashi SUZUKI, Hideyuki Proc Jpn Acad Ser B Phys Biol Sci Review L-DOPA is an amino acid that is used as a treatment for Parkinson’s disease. A simple enzymatic synthesis method of L-DOPA had been developed using bacterial L-tyrosine phenol-lyase (Tpl). This review describes research on screening of bacterial strains, culture conditions, properties of the enzyme, reaction mechanism of the enzyme, and the reaction conditions for the production of L-DOPA. Furthermore, molecular bleeding of constitutively Tpl-overproducing strains is described, which were developed based on mutations in a DNA binding protein, TyrR, which controls the induction of tpl gene expression. The Japan Academy 2023-03-10 /pmc/articles/PMC10170061/ /pubmed/36908174 http://dx.doi.org/10.2183/pjab.99.006 Text en © 2023 The Author(s). https://creativecommons.org/licenses/by-nc/4.0/Published under the terms of the CC BY-NC license https://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Review
KUMAGAI, Hidehiko
KATAYAMA, Takane
KOYANAGI, Takashi
SUZUKI, Hideyuki
Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
title Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
title_full Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
title_fullStr Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
title_full_unstemmed Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
title_short Research overview of L-DOPA production using a bacterial enzyme, tyrosine phenol-lyase
title_sort research overview of l-dopa production using a bacterial enzyme, tyrosine phenol-lyase
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170061/
https://www.ncbi.nlm.nih.gov/pubmed/36908174
http://dx.doi.org/10.2183/pjab.99.006
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