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Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma

Head and neck squamous cell carcinoma (HNSCC) is a common tumour type in otorhinolaryngology, and its occurrence is related to long-term exposure to tobacco and alcohol. Recently, HPV infection has become an increasingly important contributor to HNSCC, and HPV-associated HNSCC has a different clinic...

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Autores principales: Yanan, Li, Hui, Liang, Zhuo, Cheng, Longqing, Ding, Ran, Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170109/
https://www.ncbi.nlm.nih.gov/pubmed/37161060
http://dx.doi.org/10.1038/s41598-023-34698-4
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author Yanan, Li
Hui, Liang
Zhuo, Cheng
Longqing, Ding
Ran, Sun
author_facet Yanan, Li
Hui, Liang
Zhuo, Cheng
Longqing, Ding
Ran, Sun
author_sort Yanan, Li
collection PubMed
description Head and neck squamous cell carcinoma (HNSCC) is a common tumour type in otorhinolaryngology, and its occurrence is related to long-term exposure to tobacco and alcohol. Recently, HPV infection has become an increasingly important contributor to HNSCC, and HPV-associated HNSCC has a different clinical course and better prognosis than non-HPV-associated HNSCC. However, the exact molecular mechanism of HNSCC is unclear. Here, we obtained data from The Cancer Genome Atlas (TCGA) and gene expression omnibus (GEO) to analyse the mitophagy process and related influencing factors of HPV-associated HNSCC via the integration of bioinformatics analysis and experimental validation. We found that in HPV-associated HNSCC, process of mitophagy affects tumour development, immune cell infiltration and prognosis. In the mitophagy process of HPV-related HNSCC: NOS2, IL17REL, TMSB15A, TUBB4A and other hub genes showed significantly higher expression levels than in non-HPV-related HNSCC. Furthermore, this was also confirmed by quantitative real-time PCR (qRT‒PCR), which was used to detect the expression of differentially expressed genes in HNSCC tissues. Furthermore, we found that the unique immunological characteristics by expressing CD8(+) T cell in a high level in HPV-related HNSCC, and the scores obtained from the score model affected the prognosis of patients. In conclusion, our study revealed the unique biomolecular signature of mitophagy in HPV-associated HNSCC, which may contribute to the development of precise treatment regimens for HPV-associated HNSCC patients.
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spelling pubmed-101701092023-05-11 Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma Yanan, Li Hui, Liang Zhuo, Cheng Longqing, Ding Ran, Sun Sci Rep Article Head and neck squamous cell carcinoma (HNSCC) is a common tumour type in otorhinolaryngology, and its occurrence is related to long-term exposure to tobacco and alcohol. Recently, HPV infection has become an increasingly important contributor to HNSCC, and HPV-associated HNSCC has a different clinical course and better prognosis than non-HPV-associated HNSCC. However, the exact molecular mechanism of HNSCC is unclear. Here, we obtained data from The Cancer Genome Atlas (TCGA) and gene expression omnibus (GEO) to analyse the mitophagy process and related influencing factors of HPV-associated HNSCC via the integration of bioinformatics analysis and experimental validation. We found that in HPV-associated HNSCC, process of mitophagy affects tumour development, immune cell infiltration and prognosis. In the mitophagy process of HPV-related HNSCC: NOS2, IL17REL, TMSB15A, TUBB4A and other hub genes showed significantly higher expression levels than in non-HPV-related HNSCC. Furthermore, this was also confirmed by quantitative real-time PCR (qRT‒PCR), which was used to detect the expression of differentially expressed genes in HNSCC tissues. Furthermore, we found that the unique immunological characteristics by expressing CD8(+) T cell in a high level in HPV-related HNSCC, and the scores obtained from the score model affected the prognosis of patients. In conclusion, our study revealed the unique biomolecular signature of mitophagy in HPV-associated HNSCC, which may contribute to the development of precise treatment regimens for HPV-associated HNSCC patients. Nature Publishing Group UK 2023-05-09 /pmc/articles/PMC10170109/ /pubmed/37161060 http://dx.doi.org/10.1038/s41598-023-34698-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yanan, Li
Hui, Liang
Zhuo, Cheng
Longqing, Ding
Ran, Sun
Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma
title Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma
title_full Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma
title_fullStr Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma
title_full_unstemmed Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma
title_short Comprehensive analysis of mitophagy in HPV-related head and neck squamous cell carcinoma
title_sort comprehensive analysis of mitophagy in hpv-related head and neck squamous cell carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170109/
https://www.ncbi.nlm.nih.gov/pubmed/37161060
http://dx.doi.org/10.1038/s41598-023-34698-4
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