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The marriage of coherent Raman scattering imaging and advanced computational tools

Coherent Raman scattering microscopy can provide high-contrast tissue and single-cell images based on the inherent molecular vibrations of the sample. However, conventional techniques face a three-way trade-off between Raman spectral bandwidth, imaging speed, and image fidelity. Although currently c...

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Detalles Bibliográficos
Autores principales: Peterson, Walker, Hiramatsu, Kotaro, Goda, Keisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170129/
https://www.ncbi.nlm.nih.gov/pubmed/37160889
http://dx.doi.org/10.1038/s41377-023-01160-z
Descripción
Sumario:Coherent Raman scattering microscopy can provide high-contrast tissue and single-cell images based on the inherent molecular vibrations of the sample. However, conventional techniques face a three-way trade-off between Raman spectral bandwidth, imaging speed, and image fidelity. Although currently challenging to address via optical design, this trade-off can be overcome via emerging computational tools such as compressive sensing and machine learning.