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Colonization dynamics of a defensive insect ectosymbiont
Beneficial symbionts are horizontally or vertically transmitted to offspring, relying on host- or microbe-mediated mechanisms for colonization. While multiple studies on symbionts transmitted internally or by feeding highlight host adaptations and dynamics of symbiont colonization, less is known for...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170204/ https://www.ncbi.nlm.nih.gov/pubmed/37161294 http://dx.doi.org/10.1098/rsbl.2023.0100 |
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author | Ganesan, Ramya Janke, Rebekka S. Kaltenpoth, Martin Flórez, Laura V. |
author_facet | Ganesan, Ramya Janke, Rebekka S. Kaltenpoth, Martin Flórez, Laura V. |
author_sort | Ganesan, Ramya |
collection | PubMed |
description | Beneficial symbionts are horizontally or vertically transmitted to offspring, relying on host- or microbe-mediated mechanisms for colonization. While multiple studies on symbionts transmitted internally or by feeding highlight host adaptations and dynamics of symbiont colonization, less is known for beneficial microbes colonizing host external surfaces, such as the insect cuticle. Here, we investigate the colonization dynamics of a bacterial symbiont that protects eggs and larvae of Lagria villosa beetles against pathogens. After maternal application to the egg surface, symbionts colonize specialized cuticular invaginations on the dorsal surface of larvae. We assessed the colonization time point and investigated the involvement of the host during this process. Symbionts remain on the egg surface before hatching, providing protection. Immediately after hatching, cells from the egg surface colonize the larvae and horizontal acquisition can occur, yet efficiency decreases with increasing larval age. Additionally, passive or host-aided translocation likely supports colonization of the larval symbiotic organs. This may be especially important for the dominant non-motile symbiont strain, while motility of additional strains in the symbiont community might also play a role. Our findings provide insights into the colonization dynamics of cuticle-associated defensive symbionts and suggest alternate or complementary strategies used by different strains for colonization. |
format | Online Article Text |
id | pubmed-10170204 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-101702042023-05-11 Colonization dynamics of a defensive insect ectosymbiont Ganesan, Ramya Janke, Rebekka S. Kaltenpoth, Martin Flórez, Laura V. Biol Lett Evolutionary Biology Beneficial symbionts are horizontally or vertically transmitted to offspring, relying on host- or microbe-mediated mechanisms for colonization. While multiple studies on symbionts transmitted internally or by feeding highlight host adaptations and dynamics of symbiont colonization, less is known for beneficial microbes colonizing host external surfaces, such as the insect cuticle. Here, we investigate the colonization dynamics of a bacterial symbiont that protects eggs and larvae of Lagria villosa beetles against pathogens. After maternal application to the egg surface, symbionts colonize specialized cuticular invaginations on the dorsal surface of larvae. We assessed the colonization time point and investigated the involvement of the host during this process. Symbionts remain on the egg surface before hatching, providing protection. Immediately after hatching, cells from the egg surface colonize the larvae and horizontal acquisition can occur, yet efficiency decreases with increasing larval age. Additionally, passive or host-aided translocation likely supports colonization of the larval symbiotic organs. This may be especially important for the dominant non-motile symbiont strain, while motility of additional strains in the symbiont community might also play a role. Our findings provide insights into the colonization dynamics of cuticle-associated defensive symbionts and suggest alternate or complementary strategies used by different strains for colonization. The Royal Society 2023-05-10 /pmc/articles/PMC10170204/ /pubmed/37161294 http://dx.doi.org/10.1098/rsbl.2023.0100 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Evolutionary Biology Ganesan, Ramya Janke, Rebekka S. Kaltenpoth, Martin Flórez, Laura V. Colonization dynamics of a defensive insect ectosymbiont |
title | Colonization dynamics of a defensive insect ectosymbiont |
title_full | Colonization dynamics of a defensive insect ectosymbiont |
title_fullStr | Colonization dynamics of a defensive insect ectosymbiont |
title_full_unstemmed | Colonization dynamics of a defensive insect ectosymbiont |
title_short | Colonization dynamics of a defensive insect ectosymbiont |
title_sort | colonization dynamics of a defensive insect ectosymbiont |
topic | Evolutionary Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170204/ https://www.ncbi.nlm.nih.gov/pubmed/37161294 http://dx.doi.org/10.1098/rsbl.2023.0100 |
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