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Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients

BACKGROUND: At present, the relationship between virulence factors of Helicobacter pylori (H. pylori) and gastrointestinal diseases is still under discussion. This study investigated the association between distinct virulence factors in H. pylori and different gastrointestinal diseases. METHODS: Gas...

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Autores principales: Wang, Xiaohui, Gong, Yanan, He, Lihua, Zhao, Lu, Wang, Yiguan, Zhang, Jianzhong, Cui, Lihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170277/
https://www.ncbi.nlm.nih.gov/pubmed/37181346
http://dx.doi.org/10.21037/atm-23-1404
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author Wang, Xiaohui
Gong, Yanan
He, Lihua
Zhao, Lu
Wang, Yiguan
Zhang, Jianzhong
Cui, Lihong
author_facet Wang, Xiaohui
Gong, Yanan
He, Lihua
Zhao, Lu
Wang, Yiguan
Zhang, Jianzhong
Cui, Lihong
author_sort Wang, Xiaohui
collection PubMed
description BACKGROUND: At present, the relationship between virulence factors of Helicobacter pylori (H. pylori) and gastrointestinal diseases is still under discussion. This study investigated the association between distinct virulence factors in H. pylori and different gastrointestinal diseases. METHODS: Gastric biopsy specimens were obtained from 160 patients with different gastrointestinal diseases in China, including 77 patients with chronic gastritis (CG), 36 with peptic ulcer disease (PUD), and 38 with gastric carcinoma (GC). The presence of certain virulence genes was determined by polymerase chain reaction (PCR), and the results were analyzed using chi-squared tests. RESULTS: A total of 160 H. pylori strains were successfully isolated from gastric biopsy specimens. Overall, all strains of H. pylori were cagA, cagE positive, and the most common vacA genotypes were s1 (98.8%) and m2 (68.1%). The positive rates of the jhp0562, jhp0563, homA, homB, hopQI, and hopQII genes were 99.4%, 32.5%, 33.1%, 71.3%, 100%, and 6.9%, respectively. There was no significant association between these genes and different disease types. The dominant hpyIIIR-positive genotype was detected in 83.1% of the strains, making it significantly more prevalent than the hrgA-positive genotype (P<0.001). Surprisingly, the mixed genotype of hrgA and hpyIIIR was common and accounted for 41.3%. The hrgA-positive strains were more common in GC patients (71.1%) compared to CG patients (50.7%, P<0.05). The mixed genotype was prevalent and accounted for 55.3% and 31.2% of strains from GC and CG patients, respectively. Multivariate analysis showed that the hrgA gene exhibited a positive correlation with GC and increased the risk of GC [odds ratio (OR) =3.606, P<0.05]. In contrast, the presence of hrgA exhibited a negative correlation with CG (OR =0.499, P<0.05). CONCLUSIONS: These results suggested that the universal presence of cagA, cagE, vacA s1, jhp0562, homB, and hopQI made it impossible to examine disease-specific associations with any of these virulence factors. In addition, they may synergistically contribute to more virulent strains and severe diseases in China. Furthermore, there was a strong association between the hrgA gene and progression to GC, indicating the potential application of other virulence factors in clinical detection.
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spelling pubmed-101702772023-05-11 Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients Wang, Xiaohui Gong, Yanan He, Lihua Zhao, Lu Wang, Yiguan Zhang, Jianzhong Cui, Lihong Ann Transl Med Original Article BACKGROUND: At present, the relationship between virulence factors of Helicobacter pylori (H. pylori) and gastrointestinal diseases is still under discussion. This study investigated the association between distinct virulence factors in H. pylori and different gastrointestinal diseases. METHODS: Gastric biopsy specimens were obtained from 160 patients with different gastrointestinal diseases in China, including 77 patients with chronic gastritis (CG), 36 with peptic ulcer disease (PUD), and 38 with gastric carcinoma (GC). The presence of certain virulence genes was determined by polymerase chain reaction (PCR), and the results were analyzed using chi-squared tests. RESULTS: A total of 160 H. pylori strains were successfully isolated from gastric biopsy specimens. Overall, all strains of H. pylori were cagA, cagE positive, and the most common vacA genotypes were s1 (98.8%) and m2 (68.1%). The positive rates of the jhp0562, jhp0563, homA, homB, hopQI, and hopQII genes were 99.4%, 32.5%, 33.1%, 71.3%, 100%, and 6.9%, respectively. There was no significant association between these genes and different disease types. The dominant hpyIIIR-positive genotype was detected in 83.1% of the strains, making it significantly more prevalent than the hrgA-positive genotype (P<0.001). Surprisingly, the mixed genotype of hrgA and hpyIIIR was common and accounted for 41.3%. The hrgA-positive strains were more common in GC patients (71.1%) compared to CG patients (50.7%, P<0.05). The mixed genotype was prevalent and accounted for 55.3% and 31.2% of strains from GC and CG patients, respectively. Multivariate analysis showed that the hrgA gene exhibited a positive correlation with GC and increased the risk of GC [odds ratio (OR) =3.606, P<0.05]. In contrast, the presence of hrgA exhibited a negative correlation with CG (OR =0.499, P<0.05). CONCLUSIONS: These results suggested that the universal presence of cagA, cagE, vacA s1, jhp0562, homB, and hopQI made it impossible to examine disease-specific associations with any of these virulence factors. In addition, they may synergistically contribute to more virulent strains and severe diseases in China. Furthermore, there was a strong association between the hrgA gene and progression to GC, indicating the potential application of other virulence factors in clinical detection. AME Publishing Company 2023-04-30 2023-04-28 /pmc/articles/PMC10170277/ /pubmed/37181346 http://dx.doi.org/10.21037/atm-23-1404 Text en 2023 Annals of Translational Medicine. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Original Article
Wang, Xiaohui
Gong, Yanan
He, Lihua
Zhao, Lu
Wang, Yiguan
Zhang, Jianzhong
Cui, Lihong
Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients
title Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients
title_full Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients
title_fullStr Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients
title_full_unstemmed Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients
title_short Clinical relevance and distribution of Helicobacter pylori virulence factors in isolates from Chinese patients
title_sort clinical relevance and distribution of helicobacter pylori virulence factors in isolates from chinese patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170277/
https://www.ncbi.nlm.nih.gov/pubmed/37181346
http://dx.doi.org/10.21037/atm-23-1404
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