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Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease
Metabolic dysfunction-associated fatty liver disease (MAFLD) is a phenotype of liver diseases associated with metabolic syndrome. The pathogenesis MAFLD remains unclear. The liver maintains is located near the intestine and is physiologically interdependent with the intestine via metabolic exchange...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170973/ https://www.ncbi.nlm.nih.gov/pubmed/37180439 http://dx.doi.org/10.3389/fcimb.2023.1157368 |
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author | Niu, Chenguang Tu, Ye Jin, Qiaoqiao Chen, Zhanyi Yuan, Keyong Wang, Min Zhang, Pengfei Luo, Junyuan Li, Hao Yang, Yueyi Liu, Xiaoyu Mao, Mengying Dong, Ting Tan, Wenduo Hu, Xuchen Pan, Yihuai Hou, Lili Ma, Rui Huang, Zhengwei |
author_facet | Niu, Chenguang Tu, Ye Jin, Qiaoqiao Chen, Zhanyi Yuan, Keyong Wang, Min Zhang, Pengfei Luo, Junyuan Li, Hao Yang, Yueyi Liu, Xiaoyu Mao, Mengying Dong, Ting Tan, Wenduo Hu, Xuchen Pan, Yihuai Hou, Lili Ma, Rui Huang, Zhengwei |
author_sort | Niu, Chenguang |
collection | PubMed |
description | Metabolic dysfunction-associated fatty liver disease (MAFLD) is a phenotype of liver diseases associated with metabolic syndrome. The pathogenesis MAFLD remains unclear. The liver maintains is located near the intestine and is physiologically interdependent with the intestine via metabolic exchange and microbial transmission, underpinning the recently proposed “oral-gut-liver axis” concept. However, little is known about the roles of commensal fungi in the disease development. This study aimed to characterize the alterations of oral and gut mycobiota and their roles in MAFLD. Twenty-one MAFLD participants and 20 healthy controls were enrolled. Metagenomics analyses of saliva, supragingival plaques, and feces revealed significant alterations in the gut fungal composition of MAFLD patients. Although no statistical difference was evident in the oral mycobiome diversity within MAFLD and healthy group, significantly decreased diversities were observed in fecal samples of MAFLD patients. The relative abundance of one salivary species, five supragingival species, and seven fecal species was significantly altered in MAFLD patients. Twenty-two salivary, 23 supragingival, and 22 fecal species were associated with clinical parameters. Concerning the different functions of fungal species, pathways involved in metabolic pathways, biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and carbon metabolism were abundant both in the oral and gut mycobiomes. Moreover, different fungal contributions in core functions were observed between MAFLD patients and the healthy controls, especially in the supragingival plaque and fecal samples. Finally, correlation analysis between oral/gut mycobiome and clinical parameters identified correlations of certain fungal species in both oral and gut niches. Particularly, Mucor ambiguus, which was abundant both in saliva and feces, was positively correlated with body mass index, total cholesterol, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase, providing evidence of a possible “oral-gut-liver” axis. The findings illustrate the potential correlation between core mycobiome and the development of MAFLD and could propose potential therapeutic strategies. |
format | Online Article Text |
id | pubmed-10170973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101709732023-05-11 Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease Niu, Chenguang Tu, Ye Jin, Qiaoqiao Chen, Zhanyi Yuan, Keyong Wang, Min Zhang, Pengfei Luo, Junyuan Li, Hao Yang, Yueyi Liu, Xiaoyu Mao, Mengying Dong, Ting Tan, Wenduo Hu, Xuchen Pan, Yihuai Hou, Lili Ma, Rui Huang, Zhengwei Front Cell Infect Microbiol Cellular and Infection Microbiology Metabolic dysfunction-associated fatty liver disease (MAFLD) is a phenotype of liver diseases associated with metabolic syndrome. The pathogenesis MAFLD remains unclear. The liver maintains is located near the intestine and is physiologically interdependent with the intestine via metabolic exchange and microbial transmission, underpinning the recently proposed “oral-gut-liver axis” concept. However, little is known about the roles of commensal fungi in the disease development. This study aimed to characterize the alterations of oral and gut mycobiota and their roles in MAFLD. Twenty-one MAFLD participants and 20 healthy controls were enrolled. Metagenomics analyses of saliva, supragingival plaques, and feces revealed significant alterations in the gut fungal composition of MAFLD patients. Although no statistical difference was evident in the oral mycobiome diversity within MAFLD and healthy group, significantly decreased diversities were observed in fecal samples of MAFLD patients. The relative abundance of one salivary species, five supragingival species, and seven fecal species was significantly altered in MAFLD patients. Twenty-two salivary, 23 supragingival, and 22 fecal species were associated with clinical parameters. Concerning the different functions of fungal species, pathways involved in metabolic pathways, biosynthesis of secondary metabolites, microbial metabolism in diverse environments, and carbon metabolism were abundant both in the oral and gut mycobiomes. Moreover, different fungal contributions in core functions were observed between MAFLD patients and the healthy controls, especially in the supragingival plaque and fecal samples. Finally, correlation analysis between oral/gut mycobiome and clinical parameters identified correlations of certain fungal species in both oral and gut niches. Particularly, Mucor ambiguus, which was abundant both in saliva and feces, was positively correlated with body mass index, total cholesterol, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase, providing evidence of a possible “oral-gut-liver” axis. The findings illustrate the potential correlation between core mycobiome and the development of MAFLD and could propose potential therapeutic strategies. Frontiers Media S.A. 2023-04-26 /pmc/articles/PMC10170973/ /pubmed/37180439 http://dx.doi.org/10.3389/fcimb.2023.1157368 Text en Copyright © 2023 Niu, Tu, Jin, Chen, Yuan, Wang, Zhang, Luo, Li, Yang, Liu, Mao, Dong, Tan, Hu, Pan, Hou, Ma and Huang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Niu, Chenguang Tu, Ye Jin, Qiaoqiao Chen, Zhanyi Yuan, Keyong Wang, Min Zhang, Pengfei Luo, Junyuan Li, Hao Yang, Yueyi Liu, Xiaoyu Mao, Mengying Dong, Ting Tan, Wenduo Hu, Xuchen Pan, Yihuai Hou, Lili Ma, Rui Huang, Zhengwei Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease |
title | Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease |
title_full | Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease |
title_fullStr | Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease |
title_full_unstemmed | Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease |
title_short | Mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease |
title_sort | mapping the human oral and gut fungal microbiota in patients with metabolic dysfunction-associated fatty liver disease |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10170973/ https://www.ncbi.nlm.nih.gov/pubmed/37180439 http://dx.doi.org/10.3389/fcimb.2023.1157368 |
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