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Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes

Recently, interest in the black soldier fly larvae (BSFL) gut microbiome has received increased attention primarily due to their role in waste bioconversion. However, there is a lack of information on the positive effect on the activities of the gut microbiomes and enzymes (CAZyme families) acting o...

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Autores principales: Kariuki, Eric G., Kibet, Caleb, Paredes, Juan C., Mboowa, Gerald, Mwaura, Oscar, Njogu, John, Masiga, Daniel, Bugg, Timothy D. H., Tanga, Chrysantus M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171111/
https://www.ncbi.nlm.nih.gov/pubmed/37180276
http://dx.doi.org/10.3389/fmicb.2023.1120224
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author Kariuki, Eric G.
Kibet, Caleb
Paredes, Juan C.
Mboowa, Gerald
Mwaura, Oscar
Njogu, John
Masiga, Daniel
Bugg, Timothy D. H.
Tanga, Chrysantus M.
author_facet Kariuki, Eric G.
Kibet, Caleb
Paredes, Juan C.
Mboowa, Gerald
Mwaura, Oscar
Njogu, John
Masiga, Daniel
Bugg, Timothy D. H.
Tanga, Chrysantus M.
author_sort Kariuki, Eric G.
collection PubMed
description Recently, interest in the black soldier fly larvae (BSFL) gut microbiome has received increased attention primarily due to their role in waste bioconversion. However, there is a lack of information on the positive effect on the activities of the gut microbiomes and enzymes (CAZyme families) acting on lignocellulose. In this study, BSFL were subjected to lignocellulose-rich diets: chicken feed (CF), chicken manure (CM), brewers’ spent grain (BSG), and water hyacinth (WH). The mRNA libraries were prepared, and RNA-Sequencing was conducted using the PCR-cDNA approach through the MinION sequencing platform. Our results demonstrated that BSFL reared on BSG and WH had the highest abundance of Bacteroides and Dysgonomonas. The presence of GH51 and GH43_16 enzyme families in the gut of BSFL with both α-L-arabinofuranosidases and exo-alpha-L-arabinofuranosidase 2 were common in the BSFL reared on the highly lignocellulosic WH and BSG diets. Gene clusters that encode hemicellulolytic arabinofuranosidases in the CAZy family GH51 were also identified. These findings provide novel insight into the shift of gut microbiomes and the potential role of BSFL in the bioconversion of various highly lignocellulosic diets to fermentable sugars for subsequent value-added products (bioethanol). Further research on the role of these enzymes to improve existing technologies and their biotechnological applications is crucial.
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spelling pubmed-101711112023-05-11 Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes Kariuki, Eric G. Kibet, Caleb Paredes, Juan C. Mboowa, Gerald Mwaura, Oscar Njogu, John Masiga, Daniel Bugg, Timothy D. H. Tanga, Chrysantus M. Front Microbiol Microbiology Recently, interest in the black soldier fly larvae (BSFL) gut microbiome has received increased attention primarily due to their role in waste bioconversion. However, there is a lack of information on the positive effect on the activities of the gut microbiomes and enzymes (CAZyme families) acting on lignocellulose. In this study, BSFL were subjected to lignocellulose-rich diets: chicken feed (CF), chicken manure (CM), brewers’ spent grain (BSG), and water hyacinth (WH). The mRNA libraries were prepared, and RNA-Sequencing was conducted using the PCR-cDNA approach through the MinION sequencing platform. Our results demonstrated that BSFL reared on BSG and WH had the highest abundance of Bacteroides and Dysgonomonas. The presence of GH51 and GH43_16 enzyme families in the gut of BSFL with both α-L-arabinofuranosidases and exo-alpha-L-arabinofuranosidase 2 were common in the BSFL reared on the highly lignocellulosic WH and BSG diets. Gene clusters that encode hemicellulolytic arabinofuranosidases in the CAZy family GH51 were also identified. These findings provide novel insight into the shift of gut microbiomes and the potential role of BSFL in the bioconversion of various highly lignocellulosic diets to fermentable sugars for subsequent value-added products (bioethanol). Further research on the role of these enzymes to improve existing technologies and their biotechnological applications is crucial. Frontiers Media S.A. 2023-04-26 /pmc/articles/PMC10171111/ /pubmed/37180276 http://dx.doi.org/10.3389/fmicb.2023.1120224 Text en Copyright © 2023 Kariuki, Kibet, Paredes, Mboowa, Mwaura, Njogu, Masiga, Bugg and Tanga. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Kariuki, Eric G.
Kibet, Caleb
Paredes, Juan C.
Mboowa, Gerald
Mwaura, Oscar
Njogu, John
Masiga, Daniel
Bugg, Timothy D. H.
Tanga, Chrysantus M.
Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
title Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
title_full Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
title_fullStr Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
title_full_unstemmed Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
title_short Metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
title_sort metatranscriptomic analysis of the gut microbiome of black soldier fly larvae reared on lignocellulose-rich fiber diets unveils key lignocellulolytic enzymes
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171111/
https://www.ncbi.nlm.nih.gov/pubmed/37180276
http://dx.doi.org/10.3389/fmicb.2023.1120224
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