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In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B
The in vitro micronucleus (MNvit) assay is used to evaluate the aneugenic and clastogenic potential of a test material based upon its ability to induce micronuclei in the cells. This protocol is provided for testing of nanomaterials (NM) with standard cell lines in the absence of metabolic activatio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171196/ https://www.ncbi.nlm.nih.gov/pubmed/37180488 http://dx.doi.org/10.3389/ftox.2023.1171960 |
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author | Farabaugh, Christopher S. Doak, Shareen Roy, Shambhu Elespuru, Rosalie |
author_facet | Farabaugh, Christopher S. Doak, Shareen Roy, Shambhu Elespuru, Rosalie |
author_sort | Farabaugh, Christopher S. |
collection | PubMed |
description | The in vitro micronucleus (MNvit) assay is used to evaluate the aneugenic and clastogenic potential of a test material based upon its ability to induce micronuclei in the cells. This protocol is provided for testing of nanomaterials (NM) with standard cell lines in the absence of metabolic activation. The use of cytochalasin B (CytoB) and the analysis of binucleated cells in the cytokinesis-block version of the micronucleus assay ensures that cells analyzed have undergone cell division, which is required for expression of DNA damage and micronucleus formation. Issues specific to NM that were problematic with standard test methods are addressed, including test system choice, dose selection, test material exposures, CytoB timing, cytotoxicity determination, and DNA damage expression time. A step-by-step protocol for in vitro micronucleus assessment of NM is provided. |
format | Online Article Text |
id | pubmed-10171196 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101711962023-05-11 In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B Farabaugh, Christopher S. Doak, Shareen Roy, Shambhu Elespuru, Rosalie Front Toxicol Toxicology The in vitro micronucleus (MNvit) assay is used to evaluate the aneugenic and clastogenic potential of a test material based upon its ability to induce micronuclei in the cells. This protocol is provided for testing of nanomaterials (NM) with standard cell lines in the absence of metabolic activation. The use of cytochalasin B (CytoB) and the analysis of binucleated cells in the cytokinesis-block version of the micronucleus assay ensures that cells analyzed have undergone cell division, which is required for expression of DNA damage and micronucleus formation. Issues specific to NM that were problematic with standard test methods are addressed, including test system choice, dose selection, test material exposures, CytoB timing, cytotoxicity determination, and DNA damage expression time. A step-by-step protocol for in vitro micronucleus assessment of NM is provided. Frontiers Media S.A. 2023-04-26 /pmc/articles/PMC10171196/ /pubmed/37180488 http://dx.doi.org/10.3389/ftox.2023.1171960 Text en Copyright © 2023 Farabaugh, Doak, Roy and Elespuru. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Toxicology Farabaugh, Christopher S. Doak, Shareen Roy, Shambhu Elespuru, Rosalie In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B |
title |
In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B |
title_full |
In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B |
title_fullStr |
In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B |
title_full_unstemmed |
In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B |
title_short |
In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B |
title_sort | in vitro micronucleus assay: method for assessment of nanomaterials using cytochalasin b |
topic | Toxicology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171196/ https://www.ncbi.nlm.nih.gov/pubmed/37180488 http://dx.doi.org/10.3389/ftox.2023.1171960 |
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