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Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate

Here, we reported the complete profiling of the crotonylation proteome in common wheat. Through a combination of crotonylation and multi-omics analysis, we identified a TaPGK associated with wheat cold stress. Then, we confirmed the positive role of TaPGK-modulating wheat cold tolerance. Meanwhile,...

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Autores principales: Zhang, Ning, Wang, Sisheng, Zhao, Simin, Chen, Daiying, Tian, Hongyan, Li, Jia, Zhang, Lingran, Li, Songgang, Liu, Lu, Shi, Chaonan, Yu, Xiaodong, Ren, Yan, Chen, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171821/
https://www.ncbi.nlm.nih.gov/pubmed/37163591
http://dx.doi.org/10.1126/sciadv.adg1012
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author Zhang, Ning
Wang, Sisheng
Zhao, Simin
Chen, Daiying
Tian, Hongyan
Li, Jia
Zhang, Lingran
Li, Songgang
Liu, Lu
Shi, Chaonan
Yu, Xiaodong
Ren, Yan
Chen, Feng
author_facet Zhang, Ning
Wang, Sisheng
Zhao, Simin
Chen, Daiying
Tian, Hongyan
Li, Jia
Zhang, Lingran
Li, Songgang
Liu, Lu
Shi, Chaonan
Yu, Xiaodong
Ren, Yan
Chen, Feng
author_sort Zhang, Ning
collection PubMed
description Here, we reported the complete profiling of the crotonylation proteome in common wheat. Through a combination of crotonylation and multi-omics analysis, we identified a TaPGK associated with wheat cold stress. Then, we confirmed the positive role of TaPGK-modulating wheat cold tolerance. Meanwhile, we found that cold stress induced lysine crotonylation of TaPGK. Moreover, we screened a lysine decrotonylase TaSRT1 interacting with TaPGK and found that TaSRT1 negatively regulated wheat cold tolerance. We subsequently demonstrated TaSRT1 inhibiting the accumulation of TaPGK protein, and this inhibition was possibly resulted from decrotonylation of TaPGK by TaSRT1. Transcriptome sequencing indicated that overexpression of TaPGK activated glycolytic key genes and thereby increased pyruvate content. Moreover, we found that exogenous application of pyruvate sharply enhanced wheat cold tolerance. These findings suggest that the TaSRT1-TaPGK model regulating wheat cold tolerance is possibly through mediating pyruvate. This study provided two valuable cold tolerance genes and dissected diverse mechanism of glycolytic pathway involving in wheat cold stress.
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spelling pubmed-101718212023-05-11 Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate Zhang, Ning Wang, Sisheng Zhao, Simin Chen, Daiying Tian, Hongyan Li, Jia Zhang, Lingran Li, Songgang Liu, Lu Shi, Chaonan Yu, Xiaodong Ren, Yan Chen, Feng Sci Adv Biomedicine and Life Sciences Here, we reported the complete profiling of the crotonylation proteome in common wheat. Through a combination of crotonylation and multi-omics analysis, we identified a TaPGK associated with wheat cold stress. Then, we confirmed the positive role of TaPGK-modulating wheat cold tolerance. Meanwhile, we found that cold stress induced lysine crotonylation of TaPGK. Moreover, we screened a lysine decrotonylase TaSRT1 interacting with TaPGK and found that TaSRT1 negatively regulated wheat cold tolerance. We subsequently demonstrated TaSRT1 inhibiting the accumulation of TaPGK protein, and this inhibition was possibly resulted from decrotonylation of TaPGK by TaSRT1. Transcriptome sequencing indicated that overexpression of TaPGK activated glycolytic key genes and thereby increased pyruvate content. Moreover, we found that exogenous application of pyruvate sharply enhanced wheat cold tolerance. These findings suggest that the TaSRT1-TaPGK model regulating wheat cold tolerance is possibly through mediating pyruvate. This study provided two valuable cold tolerance genes and dissected diverse mechanism of glycolytic pathway involving in wheat cold stress. American Association for the Advancement of Science 2023-05-10 /pmc/articles/PMC10171821/ /pubmed/37163591 http://dx.doi.org/10.1126/sciadv.adg1012 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Zhang, Ning
Wang, Sisheng
Zhao, Simin
Chen, Daiying
Tian, Hongyan
Li, Jia
Zhang, Lingran
Li, Songgang
Liu, Lu
Shi, Chaonan
Yu, Xiaodong
Ren, Yan
Chen, Feng
Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
title Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
title_full Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
title_fullStr Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
title_full_unstemmed Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
title_short Global crotonylatome and GWAS revealed a TaSRT1-TaPGK model regulating wheat cold tolerance through mediating pyruvate
title_sort global crotonylatome and gwas revealed a tasrt1-tapgk model regulating wheat cold tolerance through mediating pyruvate
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10171821/
https://www.ncbi.nlm.nih.gov/pubmed/37163591
http://dx.doi.org/10.1126/sciadv.adg1012
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