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High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development

Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolutio...

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Autores principales: Bevilacqua, Carlo, Gomez, Juan Manuel, Fiuza, Ulla-Maj, Chan, Chii Jou, Wang, Ling, Hambura, Sebastian, Eguren, Manuel, Ellenberg, Jan, Diz-Muñoz, Alba, Leptin, Maria, Prevedel, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172129/
https://www.ncbi.nlm.nih.gov/pubmed/36997817
http://dx.doi.org/10.1038/s41592-023-01822-1
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author Bevilacqua, Carlo
Gomez, Juan Manuel
Fiuza, Ulla-Maj
Chan, Chii Jou
Wang, Ling
Hambura, Sebastian
Eguren, Manuel
Ellenberg, Jan
Diz-Muñoz, Alba
Leptin, Maria
Prevedel, Robert
author_facet Bevilacqua, Carlo
Gomez, Juan Manuel
Fiuza, Ulla-Maj
Chan, Chii Jou
Wang, Ling
Hambura, Sebastian
Eguren, Manuel
Ellenberg, Jan
Diz-Muñoz, Alba
Leptin, Maria
Prevedel, Robert
author_sort Bevilacqua, Carlo
collection PubMed
description Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.
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spelling pubmed-101721292023-05-12 High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development Bevilacqua, Carlo Gomez, Juan Manuel Fiuza, Ulla-Maj Chan, Chii Jou Wang, Ling Hambura, Sebastian Eguren, Manuel Ellenberg, Jan Diz-Muñoz, Alba Leptin, Maria Prevedel, Robert Nat Methods Article Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos. Nature Publishing Group US 2023-03-30 2023 /pmc/articles/PMC10172129/ /pubmed/36997817 http://dx.doi.org/10.1038/s41592-023-01822-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bevilacqua, Carlo
Gomez, Juan Manuel
Fiuza, Ulla-Maj
Chan, Chii Jou
Wang, Ling
Hambura, Sebastian
Eguren, Manuel
Ellenberg, Jan
Diz-Muñoz, Alba
Leptin, Maria
Prevedel, Robert
High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
title High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
title_full High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
title_fullStr High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
title_full_unstemmed High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
title_short High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
title_sort high-resolution line-scan brillouin microscopy for live imaging of mechanical properties during embryo development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172129/
https://www.ncbi.nlm.nih.gov/pubmed/36997817
http://dx.doi.org/10.1038/s41592-023-01822-1
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