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A light-gated cation channel with high reactivity to weak light
The cryptophyte algae, Guillardia theta, possesses 46 genes that are homologous to microbial rhodopsins. Five of them are functionally light-gated cation channelrhodopsins (GtCCR1-5) that are phylogenetically distinct from chlorophyte channelrhodopsins (ChRs) such as ChR2 from Chlamydomonas reinhard...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172181/ https://www.ncbi.nlm.nih.gov/pubmed/37165048 http://dx.doi.org/10.1038/s41598-023-34687-7 |
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author | Hososhima, Shoko Ueno, Shinji Okado, Satoshi Inoue, Ken-ichi Konno, Masae Yamauchi, Yumeka Inoue, Keiichi Terasaki, Hiroko Kandori, Hideki Tsunoda, Satoshi P. |
author_facet | Hososhima, Shoko Ueno, Shinji Okado, Satoshi Inoue, Ken-ichi Konno, Masae Yamauchi, Yumeka Inoue, Keiichi Terasaki, Hiroko Kandori, Hideki Tsunoda, Satoshi P. |
author_sort | Hososhima, Shoko |
collection | PubMed |
description | The cryptophyte algae, Guillardia theta, possesses 46 genes that are homologous to microbial rhodopsins. Five of them are functionally light-gated cation channelrhodopsins (GtCCR1-5) that are phylogenetically distinct from chlorophyte channelrhodopsins (ChRs) such as ChR2 from Chlamydomonas reinhardtii. In this study, we report the ion channel properties of these five CCRs and compared them with ChR2 and other ChRs widely used in optogenetics. We revealed that light sensitivity varied among GtCCR1-5, in which GtCCR1-3 exhibited an apparent EC(50) of 0.21–1.16 mW/mm(2), similar to that of ChR2, whereas GtCCR4 and GtCCR5 possess two EC50s, one of which is significantly small (0.025 and 0.032 mW/mm(2)). GtCCR4 is able to trigger action potentials in high temporal resolution, similar to ChR2, but requires lower light power, when expressed in cortical neurons. Moreover, a high light-sensitive response was observed when GtCCR4 was introduced into blind retina ganglion cells of rd1, a mouse model of retinitis pigmentosa. Thus, GtCCR4 provides optogenetic neuronal activation with high light sensitivity and temporal precision. |
format | Online Article Text |
id | pubmed-10172181 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-101721812023-05-12 A light-gated cation channel with high reactivity to weak light Hososhima, Shoko Ueno, Shinji Okado, Satoshi Inoue, Ken-ichi Konno, Masae Yamauchi, Yumeka Inoue, Keiichi Terasaki, Hiroko Kandori, Hideki Tsunoda, Satoshi P. Sci Rep Article The cryptophyte algae, Guillardia theta, possesses 46 genes that are homologous to microbial rhodopsins. Five of them are functionally light-gated cation channelrhodopsins (GtCCR1-5) that are phylogenetically distinct from chlorophyte channelrhodopsins (ChRs) such as ChR2 from Chlamydomonas reinhardtii. In this study, we report the ion channel properties of these five CCRs and compared them with ChR2 and other ChRs widely used in optogenetics. We revealed that light sensitivity varied among GtCCR1-5, in which GtCCR1-3 exhibited an apparent EC(50) of 0.21–1.16 mW/mm(2), similar to that of ChR2, whereas GtCCR4 and GtCCR5 possess two EC50s, one of which is significantly small (0.025 and 0.032 mW/mm(2)). GtCCR4 is able to trigger action potentials in high temporal resolution, similar to ChR2, but requires lower light power, when expressed in cortical neurons. Moreover, a high light-sensitive response was observed when GtCCR4 was introduced into blind retina ganglion cells of rd1, a mouse model of retinitis pigmentosa. Thus, GtCCR4 provides optogenetic neuronal activation with high light sensitivity and temporal precision. Nature Publishing Group UK 2023-05-10 /pmc/articles/PMC10172181/ /pubmed/37165048 http://dx.doi.org/10.1038/s41598-023-34687-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Hososhima, Shoko Ueno, Shinji Okado, Satoshi Inoue, Ken-ichi Konno, Masae Yamauchi, Yumeka Inoue, Keiichi Terasaki, Hiroko Kandori, Hideki Tsunoda, Satoshi P. A light-gated cation channel with high reactivity to weak light |
title | A light-gated cation channel with high reactivity to weak light |
title_full | A light-gated cation channel with high reactivity to weak light |
title_fullStr | A light-gated cation channel with high reactivity to weak light |
title_full_unstemmed | A light-gated cation channel with high reactivity to weak light |
title_short | A light-gated cation channel with high reactivity to weak light |
title_sort | light-gated cation channel with high reactivity to weak light |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172181/ https://www.ncbi.nlm.nih.gov/pubmed/37165048 http://dx.doi.org/10.1038/s41598-023-34687-7 |
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