Cargando…

Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis

Rickettsia and Coxiella burnetii are zoonotic tick-borne pathogens that cause febrile illnesses in humans. Metagenomic next-generation sequencing (mNGS) is a new technology used to diagnose infectious diseases. However, clinical experience with applying the test to rickettsioses and Q fever is relat...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xuan, Chen, Huixin, Han, Dongsheng, Wu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172222/
https://www.ncbi.nlm.nih.gov/pubmed/36997767
http://dx.doi.org/10.1007/s10096-023-04586-w
_version_ 1785039576576819200
author Zhang, Xuan
Chen, Huixin
Han, Dongsheng
Wu, Wei
author_facet Zhang, Xuan
Chen, Huixin
Han, Dongsheng
Wu, Wei
author_sort Zhang, Xuan
collection PubMed
description Rickettsia and Coxiella burnetii are zoonotic tick-borne pathogens that cause febrile illnesses in humans. Metagenomic next-generation sequencing (mNGS) is a new technology used to diagnose infectious diseases. However, clinical experience with applying the test to rickettsioses and Q fever is relatively limited. Therefore, this study aimed to explore the diagnostic performance of mNGS in detecting Rickettsia and C. burnetii. We retrospectively studied patients with rickettsioses or Q fever between August 2021 and July 2022. Peripheral blood mNGS and polymerase chain reaction (PCR) were performed for all patients. Clinical data were retrieved for analysis. Thirteen patients were included in this study (eleven confirmed cases and two suspected cases). Signs and symptoms included fever (13, 100%), rash (7, 53.8%), muscle soreness (5, 38.5%), headache (4, 30.8%), skin eschar (3, 23.1%), and disturbance of consciousness (2, 15.4%). In addition, eight patients (61.6%) had thrombocytopenia, ten (76.9%) had liver function impairment, and two (15.4%) had renal function impairment. The results of mNGS revealed seven patients with R. japonica (53.8%), five with C. burneti (38.5%), two with R. heilongjiangensis (15.4%), and one with R. honei (7.7%). PCR results were positive in 11 patients (84.6%). After receiving doxycycline-based treatment, 12 (92.3%) patients returned to a normal temperature within 72 h. All patients were discharged in better health. Therefore, mNGS can help diagnose Rickettsia and C. burnetii and shorten the diagnosis time, especially for patients with atypical clinical manifestations and unclear epidemiologic evidence of a tick bite or exposure.
format Online
Article
Text
id pubmed-10172222
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Springer Berlin Heidelberg
record_format MEDLINE/PubMed
spelling pubmed-101722222023-05-12 Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis Zhang, Xuan Chen, Huixin Han, Dongsheng Wu, Wei Eur J Clin Microbiol Infect Dis Original Article Rickettsia and Coxiella burnetii are zoonotic tick-borne pathogens that cause febrile illnesses in humans. Metagenomic next-generation sequencing (mNGS) is a new technology used to diagnose infectious diseases. However, clinical experience with applying the test to rickettsioses and Q fever is relatively limited. Therefore, this study aimed to explore the diagnostic performance of mNGS in detecting Rickettsia and C. burnetii. We retrospectively studied patients with rickettsioses or Q fever between August 2021 and July 2022. Peripheral blood mNGS and polymerase chain reaction (PCR) were performed for all patients. Clinical data were retrieved for analysis. Thirteen patients were included in this study (eleven confirmed cases and two suspected cases). Signs and symptoms included fever (13, 100%), rash (7, 53.8%), muscle soreness (5, 38.5%), headache (4, 30.8%), skin eschar (3, 23.1%), and disturbance of consciousness (2, 15.4%). In addition, eight patients (61.6%) had thrombocytopenia, ten (76.9%) had liver function impairment, and two (15.4%) had renal function impairment. The results of mNGS revealed seven patients with R. japonica (53.8%), five with C. burneti (38.5%), two with R. heilongjiangensis (15.4%), and one with R. honei (7.7%). PCR results were positive in 11 patients (84.6%). After receiving doxycycline-based treatment, 12 (92.3%) patients returned to a normal temperature within 72 h. All patients were discharged in better health. Therefore, mNGS can help diagnose Rickettsia and C. burnetii and shorten the diagnosis time, especially for patients with atypical clinical manifestations and unclear epidemiologic evidence of a tick bite or exposure. Springer Berlin Heidelberg 2023-03-30 2023 /pmc/articles/PMC10172222/ /pubmed/36997767 http://dx.doi.org/10.1007/s10096-023-04586-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Zhang, Xuan
Chen, Huixin
Han, Dongsheng
Wu, Wei
Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
title Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
title_full Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
title_fullStr Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
title_full_unstemmed Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
title_short Clinical usefulness of metagenomic next-generation sequencing for Rickettsia and Coxiella burnetii diagnosis
title_sort clinical usefulness of metagenomic next-generation sequencing for rickettsia and coxiella burnetii diagnosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10172222/
https://www.ncbi.nlm.nih.gov/pubmed/36997767
http://dx.doi.org/10.1007/s10096-023-04586-w
work_keys_str_mv AT zhangxuan clinicalusefulnessofmetagenomicnextgenerationsequencingforrickettsiaandcoxiellaburnetiidiagnosis
AT chenhuixin clinicalusefulnessofmetagenomicnextgenerationsequencingforrickettsiaandcoxiellaburnetiidiagnosis
AT handongsheng clinicalusefulnessofmetagenomicnextgenerationsequencingforrickettsiaandcoxiellaburnetiidiagnosis
AT wuwei clinicalusefulnessofmetagenomicnextgenerationsequencingforrickettsiaandcoxiellaburnetiidiagnosis