Competitive PCR with dual fluorescent primers enhances the specificity and reproducibility of genotyping animals generated from genome editing

Targeted genome editing is a powerful tool for studying gene function in almost every aspect of biological and pathological processes. The most widely used genome editing approach is to introduce engineered endonucleases or CRISPR/Cas system into cells or fertilized eggs to generate double-strand DN...

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Detalles Bibliográficos
Autores principales: Fu, Liezhen, Ma, Emily, Okada, Morihiro, Shibata, Yuki, Shi, Yun-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173569/
https://www.ncbi.nlm.nih.gov/pubmed/37170319
http://dx.doi.org/10.1186/s13578-023-01042-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173569/
https://www.ncbi.nlm.nih.gov/pubmed/37170319
http://dx.doi.org/10.1186/s13578-023-01042-2

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