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Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study

BACKGROUND: Recently, for various reasons, the need for non-invasive treatment for localized fat has emerged. This study confirmed whether Magnolia officinalis (MO) pharmacopuncture reduces localized fat by promoting lipolysis and inhibiting adipogenesis. METHODS: The network was built using genes r...

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Autores principales: Choi, Won Jun, Kim, Mi Hye, Park, Nayoung, Chung, Jae Yoon, Park, Sang Jun, Yang, Woong Mo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173613/
https://www.ncbi.nlm.nih.gov/pubmed/37181416
http://dx.doi.org/10.1016/j.imr.2023.100948
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author Choi, Won Jun
Kim, Mi Hye
Park, Nayoung
Chung, Jae Yoon
Park, Sang Jun
Yang, Woong Mo
author_facet Choi, Won Jun
Kim, Mi Hye
Park, Nayoung
Chung, Jae Yoon
Park, Sang Jun
Yang, Woong Mo
author_sort Choi, Won Jun
collection PubMed
description BACKGROUND: Recently, for various reasons, the need for non-invasive treatment for localized fat has emerged. This study confirmed whether Magnolia officinalis (MO) pharmacopuncture reduces localized fat by promoting lipolysis and inhibiting adipogenesis. METHODS: The network was built using genes related to the active compound of MO and the mode of action of MO was predicted by the functional enrichment analysis. Based on the result from network analysis, 100 µL of 2 mg/mL MO pharmacopuncture was injected into the inguinal fat pad for 6 weeks in obese C57BL/6J mice. Normal saline was injected into the right-side inguinal fat pad as a self-control. RESULTS: It was expected that the ‘AMP-activated protein kinase (AMPK) signaling pathway’ would be affected by the MO Network. MO pharmacopuncture reduced the weight and size of inguinal fat in HFD-induced obese mice. The phosphorylation of AMPK along with the increases of lipases was significantly increased by MO injection. Also, the expression levels of fatty acid synthesize-related mediators were suppressed by MO injection. CONCLUSION: Our results demonstrated that MO pharmacopuncture promoted the expression of AMPK, which has beneficial effects on activation of lipolysis and inhibition of lipogenesis. Pharmacopuncture of MO can be a non-surgical alternative therapy in the treatment of local fat tissue.
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spelling pubmed-101736132023-05-12 Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study Choi, Won Jun Kim, Mi Hye Park, Nayoung Chung, Jae Yoon Park, Sang Jun Yang, Woong Mo Integr Med Res Original Article BACKGROUND: Recently, for various reasons, the need for non-invasive treatment for localized fat has emerged. This study confirmed whether Magnolia officinalis (MO) pharmacopuncture reduces localized fat by promoting lipolysis and inhibiting adipogenesis. METHODS: The network was built using genes related to the active compound of MO and the mode of action of MO was predicted by the functional enrichment analysis. Based on the result from network analysis, 100 µL of 2 mg/mL MO pharmacopuncture was injected into the inguinal fat pad for 6 weeks in obese C57BL/6J mice. Normal saline was injected into the right-side inguinal fat pad as a self-control. RESULTS: It was expected that the ‘AMP-activated protein kinase (AMPK) signaling pathway’ would be affected by the MO Network. MO pharmacopuncture reduced the weight and size of inguinal fat in HFD-induced obese mice. The phosphorylation of AMPK along with the increases of lipases was significantly increased by MO injection. Also, the expression levels of fatty acid synthesize-related mediators were suppressed by MO injection. CONCLUSION: Our results demonstrated that MO pharmacopuncture promoted the expression of AMPK, which has beneficial effects on activation of lipolysis and inhibition of lipogenesis. Pharmacopuncture of MO can be a non-surgical alternative therapy in the treatment of local fat tissue. Elsevier 2023-06 2023-04-07 /pmc/articles/PMC10173613/ /pubmed/37181416 http://dx.doi.org/10.1016/j.imr.2023.100948 Text en © 2023 Korea Institute of Oriental Medicine. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Choi, Won Jun
Kim, Mi Hye
Park, Nayoung
Chung, Jae Yoon
Park, Sang Jun
Yang, Woong Mo
Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study
title Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study
title_full Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study
title_fullStr Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study
title_full_unstemmed Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study
title_short Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study
title_sort effect and mechanism of magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173613/
https://www.ncbi.nlm.nih.gov/pubmed/37181416
http://dx.doi.org/10.1016/j.imr.2023.100948
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