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Protocol for casein kinase 1γ3 CSNK1G3 gene knockout and recombinant gene expression in cultured HeLa cells
Casein kinase 1γ is a subfamily of the casein kinase 1 family. Here, we present a protocol for gene knockout of CSNK1G3 in human cells and cloning and/or expression of CSNK1G1–3 cDNAs using a retroviral vector system. We first describe the genome editing procedures, including sgRNA design, introduct...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10173854/ https://www.ncbi.nlm.nih.gov/pubmed/37119140 http://dx.doi.org/10.1016/j.xpro.2023.102251 |