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Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic

The occurrence of colistin resistance has increased rapidly among Enterobacterales around the world. We performed a national survey of plasmid-mediated colistin resistance in human clinical isolates through a retrospective analysis of samples from 2009 to 2017 and a prospective sampling in 2018–2020...

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Autores principales: Zelendova, Marketa, Papagiannitsis, Costas C., Sismova, Petra, Medvecky, Matej, Pomorska, Katarina, Palkovicova, Jana, Nesporova, Kristina, Jakubu, Vladislav, Jamborova, Ivana, Zemlickova, Helena, Dolejska, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174314/
https://www.ncbi.nlm.nih.gov/pubmed/37180238
http://dx.doi.org/10.3389/fmicb.2023.1147846
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author Zelendova, Marketa
Papagiannitsis, Costas C.
Sismova, Petra
Medvecky, Matej
Pomorska, Katarina
Palkovicova, Jana
Nesporova, Kristina
Jakubu, Vladislav
Jamborova, Ivana
Zemlickova, Helena
Dolejska, Monika
author_facet Zelendova, Marketa
Papagiannitsis, Costas C.
Sismova, Petra
Medvecky, Matej
Pomorska, Katarina
Palkovicova, Jana
Nesporova, Kristina
Jakubu, Vladislav
Jamborova, Ivana
Zemlickova, Helena
Dolejska, Monika
author_sort Zelendova, Marketa
collection PubMed
description The occurrence of colistin resistance has increased rapidly among Enterobacterales around the world. We performed a national survey of plasmid-mediated colistin resistance in human clinical isolates through a retrospective analysis of samples from 2009 to 2017 and a prospective sampling in 2018–2020. The aim of this study was to identify and characterize isolates with mcr genes from various regions of the Czech Republic using whole genome sequencing (WGS). Of all 1932 colistin-resistant isolates analyzed, 73 (3.8%) were positive for mcr genes. Most isolates carried mcr-1 (48/73) and were identified as Escherichia coli (n = 44) and Klebsiella pneumoniae (n = 4) of various sequence types (ST). Twenty-five isolates, including Enterobacter spp. (n = 24) and Citrobacter freundii (n = 1) carrying the mcr-9 gene were detected; three of them (Enterobacter kobei ST54) co-harbored the mcr-4 and mcr-9 genes. Multi-drug resistance phenotype was a common feature of mcr isolates and 14% (10/73) isolates also co-harbored clinically important beta-lactamases, including two isolates with carbapenemases KPC-2 and OXA-48. Phylogenetic analysis of E. coli ST744, the dominant genotype in this study, with the global collection showed Czech isolates belonged to two major clades, one containing isolates from Europe, while the second composed of isolates from diverse geographical areas. The mcr-1 gene was carried by IncX4 (34/73, 47%), IncHI2/ST4 (6/73, 8%) and IncI2 (8/73, 11%) plasmid groups. Small plasmids belonging to the ColE10 group were associated with mcr-4 in three isolates, while mcr-9 was carried by IncHI2/ST1 plasmids (4/73, 5%) or the chromosome (18/73, 25%). We showed an overall low level of occurrence of mcr genes in colistin-resistant bacteria from human clinical samples in the Czech Republic.
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spelling pubmed-101743142023-05-12 Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic Zelendova, Marketa Papagiannitsis, Costas C. Sismova, Petra Medvecky, Matej Pomorska, Katarina Palkovicova, Jana Nesporova, Kristina Jakubu, Vladislav Jamborova, Ivana Zemlickova, Helena Dolejska, Monika Front Microbiol Microbiology The occurrence of colistin resistance has increased rapidly among Enterobacterales around the world. We performed a national survey of plasmid-mediated colistin resistance in human clinical isolates through a retrospective analysis of samples from 2009 to 2017 and a prospective sampling in 2018–2020. The aim of this study was to identify and characterize isolates with mcr genes from various regions of the Czech Republic using whole genome sequencing (WGS). Of all 1932 colistin-resistant isolates analyzed, 73 (3.8%) were positive for mcr genes. Most isolates carried mcr-1 (48/73) and were identified as Escherichia coli (n = 44) and Klebsiella pneumoniae (n = 4) of various sequence types (ST). Twenty-five isolates, including Enterobacter spp. (n = 24) and Citrobacter freundii (n = 1) carrying the mcr-9 gene were detected; three of them (Enterobacter kobei ST54) co-harbored the mcr-4 and mcr-9 genes. Multi-drug resistance phenotype was a common feature of mcr isolates and 14% (10/73) isolates also co-harbored clinically important beta-lactamases, including two isolates with carbapenemases KPC-2 and OXA-48. Phylogenetic analysis of E. coli ST744, the dominant genotype in this study, with the global collection showed Czech isolates belonged to two major clades, one containing isolates from Europe, while the second composed of isolates from diverse geographical areas. The mcr-1 gene was carried by IncX4 (34/73, 47%), IncHI2/ST4 (6/73, 8%) and IncI2 (8/73, 11%) plasmid groups. Small plasmids belonging to the ColE10 group were associated with mcr-4 in three isolates, while mcr-9 was carried by IncHI2/ST1 plasmids (4/73, 5%) or the chromosome (18/73, 25%). We showed an overall low level of occurrence of mcr genes in colistin-resistant bacteria from human clinical samples in the Czech Republic. Frontiers Media S.A. 2023-04-27 /pmc/articles/PMC10174314/ /pubmed/37180238 http://dx.doi.org/10.3389/fmicb.2023.1147846 Text en Copyright © 2023 Zelendova, Papagiannitsis, Sismova, Medvecky, Pomorska, Palkovicova, Nesporova, Jakubu, Jamborova, Zemlickova, Dolejska and Working Group for Monitoring of Antibiotic Resistance. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zelendova, Marketa
Papagiannitsis, Costas C.
Sismova, Petra
Medvecky, Matej
Pomorska, Katarina
Palkovicova, Jana
Nesporova, Kristina
Jakubu, Vladislav
Jamborova, Ivana
Zemlickova, Helena
Dolejska, Monika
Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic
title Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic
title_full Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic
title_fullStr Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic
title_full_unstemmed Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic
title_short Plasmid-mediated colistin resistance among human clinical Enterobacterales isolates: national surveillance in the Czech Republic
title_sort plasmid-mediated colistin resistance among human clinical enterobacterales isolates: national surveillance in the czech republic
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174314/
https://www.ncbi.nlm.nih.gov/pubmed/37180238
http://dx.doi.org/10.3389/fmicb.2023.1147846
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