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Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats

OBJECTIVE: The aim of this study was to evaluate the effectiveness of hand debridement (HD) versus ultrasonic dental scaler (UDS) for the treatment of experimental periodontitis (EP) in rats. MATERIAL AND METHODS: Thirty 3‐month‐old male rats were used. EP was induced around the mandibular first mol...

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Autores principales: de Almeida, Juliano Milanezi, de Araujo, Nathália Januario, Matheus, Henrique Rinaldi, de Abreu Furquim, Elisa Mara, Piovezan, Bianca Rafaeli, Fiorin, Luiz Guilherme, Ervolino, Edilson
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174520/
https://www.ncbi.nlm.nih.gov/pubmed/37167233
http://dx.doi.org/10.1371/journal.pone.0284497
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author de Almeida, Juliano Milanezi
de Araujo, Nathália Januario
Matheus, Henrique Rinaldi
de Abreu Furquim, Elisa Mara
Piovezan, Bianca Rafaeli
Fiorin, Luiz Guilherme
Ervolino, Edilson
author_facet de Almeida, Juliano Milanezi
de Araujo, Nathália Januario
Matheus, Henrique Rinaldi
de Abreu Furquim, Elisa Mara
Piovezan, Bianca Rafaeli
Fiorin, Luiz Guilherme
Ervolino, Edilson
author_sort de Almeida, Juliano Milanezi
collection PubMed
description OBJECTIVE: The aim of this study was to evaluate the effectiveness of hand debridement (HD) versus ultrasonic dental scaler (UDS) for the treatment of experimental periodontitis (EP) in rats. MATERIAL AND METHODS: Thirty 3‐month‐old male rats were used. EP was induced around the mandibular first molars (right and left). Seven days after induction, the treatments with either HD (n = 30) or UDS (n = 30) were randomly performed in each molar. Euthanasia were performed at 7, 15, and 30 days after treatment. Histometric (percentage of bone in the furcation [PBF]), histopathological, and immunohistochemical (for detection of tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]). Parametric data (PBF and TRAP) was analyzed by One-way ANOVA followed by Tukey’s post-test. OCN was analyzed by Kruskal-Wallis followed by Student-Newman-Keuls post-test. The level of significance was 5%. RESULTS: Group HD presented higher PBF and lower TRAP-immunolabeling at 30 days as compared with UDS in the same period (p≤0.05). Group HD presented higher OCN immunolabeling at 30 days as compared with 7 and 15 days (p≤0.05). Persistent and exacerbated inflammatory process was observed in some specimens from group UDS at 30 days, as well as the bone trabeculae presented irregular contour, surrounded by many active osteoclasts. CONCLUSION: Nonsurgical periodontal therapy with HD resulted in higher PBF and lower expression of TRAP as compared with UDS. Also, HD increased the expression of OCN over time.
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spelling pubmed-101745202023-05-12 Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats de Almeida, Juliano Milanezi de Araujo, Nathália Januario Matheus, Henrique Rinaldi de Abreu Furquim, Elisa Mara Piovezan, Bianca Rafaeli Fiorin, Luiz Guilherme Ervolino, Edilson PLoS One Research Article OBJECTIVE: The aim of this study was to evaluate the effectiveness of hand debridement (HD) versus ultrasonic dental scaler (UDS) for the treatment of experimental periodontitis (EP) in rats. MATERIAL AND METHODS: Thirty 3‐month‐old male rats were used. EP was induced around the mandibular first molars (right and left). Seven days after induction, the treatments with either HD (n = 30) or UDS (n = 30) were randomly performed in each molar. Euthanasia were performed at 7, 15, and 30 days after treatment. Histometric (percentage of bone in the furcation [PBF]), histopathological, and immunohistochemical (for detection of tartrate-resistant acid phosphatase [TRAP] and osteocalcin [OCN]). Parametric data (PBF and TRAP) was analyzed by One-way ANOVA followed by Tukey’s post-test. OCN was analyzed by Kruskal-Wallis followed by Student-Newman-Keuls post-test. The level of significance was 5%. RESULTS: Group HD presented higher PBF and lower TRAP-immunolabeling at 30 days as compared with UDS in the same period (p≤0.05). Group HD presented higher OCN immunolabeling at 30 days as compared with 7 and 15 days (p≤0.05). Persistent and exacerbated inflammatory process was observed in some specimens from group UDS at 30 days, as well as the bone trabeculae presented irregular contour, surrounded by many active osteoclasts. CONCLUSION: Nonsurgical periodontal therapy with HD resulted in higher PBF and lower expression of TRAP as compared with UDS. Also, HD increased the expression of OCN over time. Public Library of Science 2023-05-11 /pmc/articles/PMC10174520/ /pubmed/37167233 http://dx.doi.org/10.1371/journal.pone.0284497 Text en © 2023 de Almeida et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
de Almeida, Juliano Milanezi
de Araujo, Nathália Januario
Matheus, Henrique Rinaldi
de Abreu Furquim, Elisa Mara
Piovezan, Bianca Rafaeli
Fiorin, Luiz Guilherme
Ervolino, Edilson
Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats
title Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats
title_full Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats
title_fullStr Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats
title_full_unstemmed Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats
title_short Hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: An in vivo study in rats
title_sort hand instrumentation provides improved tissue response over ultrasonic scaler and substantiates safe dental practice: an in vivo study in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174520/
https://www.ncbi.nlm.nih.gov/pubmed/37167233
http://dx.doi.org/10.1371/journal.pone.0284497
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