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Single-cell transcriptomics of a dynamic cell behavior in murine airways

Despite advances in high-dimensional cellular analysis, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method that allows us to couple the physiological behaviors of cells in an intact murine tissue to deep molecular profilin...

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Detalles Bibliográficos
Autores principales: Kwok, Sheldon JJ, Montoro, Daniel T, Haber, Adam L, Yun, Seok-Hyun, Vinarsky, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174686/
https://www.ncbi.nlm.nih.gov/pubmed/37083555
http://dx.doi.org/10.7554/eLife.76645
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author Kwok, Sheldon JJ
Montoro, Daniel T
Haber, Adam L
Yun, Seok-Hyun
Vinarsky, Vladimir
author_facet Kwok, Sheldon JJ
Montoro, Daniel T
Haber, Adam L
Yun, Seok-Hyun
Vinarsky, Vladimir
author_sort Kwok, Sheldon JJ
collection PubMed
description Despite advances in high-dimensional cellular analysis, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method that allows us to couple the physiological behaviors of cells in an intact murine tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following injury in murine airways.
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spelling pubmed-101746862023-05-12 Single-cell transcriptomics of a dynamic cell behavior in murine airways Kwok, Sheldon JJ Montoro, Daniel T Haber, Adam L Yun, Seok-Hyun Vinarsky, Vladimir eLife Developmental Biology Despite advances in high-dimensional cellular analysis, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method that allows us to couple the physiological behaviors of cells in an intact murine tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following injury in murine airways. eLife Sciences Publications, Ltd 2023-04-21 /pmc/articles/PMC10174686/ /pubmed/37083555 http://dx.doi.org/10.7554/eLife.76645 Text en © 2023, Kwok, Montoro, Haber et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology
Kwok, Sheldon JJ
Montoro, Daniel T
Haber, Adam L
Yun, Seok-Hyun
Vinarsky, Vladimir
Single-cell transcriptomics of a dynamic cell behavior in murine airways
title Single-cell transcriptomics of a dynamic cell behavior in murine airways
title_full Single-cell transcriptomics of a dynamic cell behavior in murine airways
title_fullStr Single-cell transcriptomics of a dynamic cell behavior in murine airways
title_full_unstemmed Single-cell transcriptomics of a dynamic cell behavior in murine airways
title_short Single-cell transcriptomics of a dynamic cell behavior in murine airways
title_sort single-cell transcriptomics of a dynamic cell behavior in murine airways
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174686/
https://www.ncbi.nlm.nih.gov/pubmed/37083555
http://dx.doi.org/10.7554/eLife.76645
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