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RNA Polymerase II transcription independent of TBP in murine embryonic stem cells

Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation. Here, we report instead that acute depletion o...

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Autores principales: Kwan, James ZJ, Nguyen, Thomas F, Uzozie, Anuli C, Budzynski, Marek A, Cui, Jieying, Lee, Joseph MC, Van Petegem, Filip, Lange, Philipp F, Teves, Sheila S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174690/
https://www.ncbi.nlm.nih.gov/pubmed/36995326
http://dx.doi.org/10.7554/eLife.83810
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author Kwan, James ZJ
Nguyen, Thomas F
Uzozie, Anuli C
Budzynski, Marek A
Cui, Jieying
Lee, Joseph MC
Van Petegem, Filip
Lange, Philipp F
Teves, Sheila S
author_facet Kwan, James ZJ
Nguyen, Thomas F
Uzozie, Anuli C
Budzynski, Marek A
Cui, Jieying
Lee, Joseph MC
Van Petegem, Filip
Lange, Philipp F
Teves, Sheila S
author_sort Kwan, James ZJ
collection PubMed
description Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation. Here, we report instead that acute depletion of TBP in mouse embryonic stem cells has no global effect on ongoing Pol II transcription. In contrast, acute TBP depletion severely impairs RNA Polymerase III initiation. Furthermore, Pol II transcriptional induction occurs normally upon TBP depletion. This TBP-independent transcription mechanism is not due to a functional redundancy with the TBP paralog TRF2, though TRF2 also binds to promoters of transcribed genes. Rather, we show that the TFIID complex can form and, despite having reduced TAF4 and TFIIA binding when TBP is depleted, the Pol II machinery is sufficiently robust in sustaining TBP-independent transcription.
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spelling pubmed-101746902023-05-12 RNA Polymerase II transcription independent of TBP in murine embryonic stem cells Kwan, James ZJ Nguyen, Thomas F Uzozie, Anuli C Budzynski, Marek A Cui, Jieying Lee, Joseph MC Van Petegem, Filip Lange, Philipp F Teves, Sheila S eLife Chromosomes and Gene Expression Transcription by RNA Polymerase II (Pol II) is initiated by the hierarchical assembly of the pre-initiation complex onto promoter DNA. Decades of research have shown that the TATA-box binding protein (TBP) is essential for Pol II loading and initiation. Here, we report instead that acute depletion of TBP in mouse embryonic stem cells has no global effect on ongoing Pol II transcription. In contrast, acute TBP depletion severely impairs RNA Polymerase III initiation. Furthermore, Pol II transcriptional induction occurs normally upon TBP depletion. This TBP-independent transcription mechanism is not due to a functional redundancy with the TBP paralog TRF2, though TRF2 also binds to promoters of transcribed genes. Rather, we show that the TFIID complex can form and, despite having reduced TAF4 and TFIIA binding when TBP is depleted, the Pol II machinery is sufficiently robust in sustaining TBP-independent transcription. eLife Sciences Publications, Ltd 2023-03-30 /pmc/articles/PMC10174690/ /pubmed/36995326 http://dx.doi.org/10.7554/eLife.83810 Text en © 2023, Kwan, Nguyen et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Chromosomes and Gene Expression
Kwan, James ZJ
Nguyen, Thomas F
Uzozie, Anuli C
Budzynski, Marek A
Cui, Jieying
Lee, Joseph MC
Van Petegem, Filip
Lange, Philipp F
Teves, Sheila S
RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
title RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
title_full RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
title_fullStr RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
title_full_unstemmed RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
title_short RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
title_sort rna polymerase ii transcription independent of tbp in murine embryonic stem cells
topic Chromosomes and Gene Expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10174690/
https://www.ncbi.nlm.nih.gov/pubmed/36995326
http://dx.doi.org/10.7554/eLife.83810
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