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CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
ABSTRACT: Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that we...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175313/ https://www.ncbi.nlm.nih.gov/pubmed/37133800 http://dx.doi.org/10.1007/s00253-023-12547-8 |
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author | Oliveira, Hugo Santos, Sílvio Pires, Diana P. Boeckaerts, Dimitri Pinto, Graça Domingues, Rita Otero, Jennifer Briers, Yves Lavigne, Rob Schmelcher, Mathias Dötsch, Andreas Azeredo, Joana |
author_facet | Oliveira, Hugo Santos, Sílvio Pires, Diana P. Boeckaerts, Dimitri Pinto, Graça Domingues, Rita Otero, Jennifer Briers, Yves Lavigne, Rob Schmelcher, Mathias Dötsch, Andreas Azeredo, Joana |
author_sort | Oliveira, Hugo |
collection | PubMed |
description | ABSTRACT: Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequence similarity with the Salmonella phage S16. Based on surface plasmon resonance and recombinant green florescence protein fusions, the tail fiber (gp267) was shown to decorate C. koseri cells, binding with a nanomolar affinity, without the need of accessory proteins. Both phage and the tail fiber specifically bind to bacterial cells by the lipopolysaccharide polymer. We further demonstrate that CkP1 is highly stable towards different environmental conditions of pH and temperatures and is able to control C. koseri cells in urine samples. Altogether, CkP1 features optimal in vitro characteristics to be used both as a control and detection agent towards drug-resistant C. koseri infections. KEY POINTS: • CkP1 infects all C. koseri strains tested • CkP1 recognizes C. koseri lipopolysaccharide through its long tail fiber • Both phage CkP1 and its tail fiber can be used to treat or detect C. koseri pathogens SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-023-12547-8. |
format | Online Article Text |
id | pubmed-10175313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-101753132023-05-13 CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers Oliveira, Hugo Santos, Sílvio Pires, Diana P. Boeckaerts, Dimitri Pinto, Graça Domingues, Rita Otero, Jennifer Briers, Yves Lavigne, Rob Schmelcher, Mathias Dötsch, Andreas Azeredo, Joana Appl Microbiol Biotechnol Applied Genetics and Molecular Biotechnology ABSTRACT: Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequence similarity with the Salmonella phage S16. Based on surface plasmon resonance and recombinant green florescence protein fusions, the tail fiber (gp267) was shown to decorate C. koseri cells, binding with a nanomolar affinity, without the need of accessory proteins. Both phage and the tail fiber specifically bind to bacterial cells by the lipopolysaccharide polymer. We further demonstrate that CkP1 is highly stable towards different environmental conditions of pH and temperatures and is able to control C. koseri cells in urine samples. Altogether, CkP1 features optimal in vitro characteristics to be used both as a control and detection agent towards drug-resistant C. koseri infections. KEY POINTS: • CkP1 infects all C. koseri strains tested • CkP1 recognizes C. koseri lipopolysaccharide through its long tail fiber • Both phage CkP1 and its tail fiber can be used to treat or detect C. koseri pathogens SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-023-12547-8. Springer Berlin Heidelberg 2023-05-03 2023 /pmc/articles/PMC10175313/ /pubmed/37133800 http://dx.doi.org/10.1007/s00253-023-12547-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Applied Genetics and Molecular Biotechnology Oliveira, Hugo Santos, Sílvio Pires, Diana P. Boeckaerts, Dimitri Pinto, Graça Domingues, Rita Otero, Jennifer Briers, Yves Lavigne, Rob Schmelcher, Mathias Dötsch, Andreas Azeredo, Joana CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers |
title | CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers |
title_full | CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers |
title_fullStr | CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers |
title_full_unstemmed | CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers |
title_short | CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers |
title_sort | ckp1 bacteriophage, a s16-like myovirus that recognizes citrobacter koseri lipopolysaccharide through its long tail fibers |
topic | Applied Genetics and Molecular Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175313/ https://www.ncbi.nlm.nih.gov/pubmed/37133800 http://dx.doi.org/10.1007/s00253-023-12547-8 |
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