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CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers

ABSTRACT: Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that we...

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Autores principales: Oliveira, Hugo, Santos, Sílvio, Pires, Diana P., Boeckaerts, Dimitri, Pinto, Graça, Domingues, Rita, Otero, Jennifer, Briers, Yves, Lavigne, Rob, Schmelcher, Mathias, Dötsch, Andreas, Azeredo, Joana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175313/
https://www.ncbi.nlm.nih.gov/pubmed/37133800
http://dx.doi.org/10.1007/s00253-023-12547-8
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author Oliveira, Hugo
Santos, Sílvio
Pires, Diana P.
Boeckaerts, Dimitri
Pinto, Graça
Domingues, Rita
Otero, Jennifer
Briers, Yves
Lavigne, Rob
Schmelcher, Mathias
Dötsch, Andreas
Azeredo, Joana
author_facet Oliveira, Hugo
Santos, Sílvio
Pires, Diana P.
Boeckaerts, Dimitri
Pinto, Graça
Domingues, Rita
Otero, Jennifer
Briers, Yves
Lavigne, Rob
Schmelcher, Mathias
Dötsch, Andreas
Azeredo, Joana
author_sort Oliveira, Hugo
collection PubMed
description ABSTRACT: Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequence similarity with the Salmonella phage S16. Based on surface plasmon resonance and recombinant green florescence protein fusions, the tail fiber (gp267) was shown to decorate C. koseri cells, binding with a nanomolar affinity, without the need of accessory proteins. Both phage and the tail fiber specifically bind to bacterial cells by the lipopolysaccharide polymer. We further demonstrate that CkP1 is highly stable towards different environmental conditions of pH and temperatures and is able to control C. koseri cells in urine samples. Altogether, CkP1 features optimal in vitro characteristics to be used both as a control and detection agent towards drug-resistant C. koseri infections. KEY POINTS: • CkP1 infects all C. koseri strains tested • CkP1 recognizes C. koseri lipopolysaccharide through its long tail fiber • Both phage CkP1 and its tail fiber can be used to treat or detect C. koseri pathogens SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-023-12547-8.
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spelling pubmed-101753132023-05-13 CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers Oliveira, Hugo Santos, Sílvio Pires, Diana P. Boeckaerts, Dimitri Pinto, Graça Domingues, Rita Otero, Jennifer Briers, Yves Lavigne, Rob Schmelcher, Mathias Dötsch, Andreas Azeredo, Joana Appl Microbiol Biotechnol Applied Genetics and Molecular Biotechnology ABSTRACT: Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri. CkP1 has a host range covering the whole C. koseri species, i.e., all strains that were tested, but does not infect other species. Its linear 168,463-bp genome contains 291 coding sequences, sharing sequence similarity with the Salmonella phage S16. Based on surface plasmon resonance and recombinant green florescence protein fusions, the tail fiber (gp267) was shown to decorate C. koseri cells, binding with a nanomolar affinity, without the need of accessory proteins. Both phage and the tail fiber specifically bind to bacterial cells by the lipopolysaccharide polymer. We further demonstrate that CkP1 is highly stable towards different environmental conditions of pH and temperatures and is able to control C. koseri cells in urine samples. Altogether, CkP1 features optimal in vitro characteristics to be used both as a control and detection agent towards drug-resistant C. koseri infections. KEY POINTS: • CkP1 infects all C. koseri strains tested • CkP1 recognizes C. koseri lipopolysaccharide through its long tail fiber • Both phage CkP1 and its tail fiber can be used to treat or detect C. koseri pathogens SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-023-12547-8. Springer Berlin Heidelberg 2023-05-03 2023 /pmc/articles/PMC10175313/ /pubmed/37133800 http://dx.doi.org/10.1007/s00253-023-12547-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Applied Genetics and Molecular Biotechnology
Oliveira, Hugo
Santos, Sílvio
Pires, Diana P.
Boeckaerts, Dimitri
Pinto, Graça
Domingues, Rita
Otero, Jennifer
Briers, Yves
Lavigne, Rob
Schmelcher, Mathias
Dötsch, Andreas
Azeredo, Joana
CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
title CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
title_full CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
title_fullStr CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
title_full_unstemmed CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
title_short CkP1 bacteriophage, a S16-like myovirus that recognizes Citrobacter koseri lipopolysaccharide through its long tail fibers
title_sort ckp1 bacteriophage, a s16-like myovirus that recognizes citrobacter koseri lipopolysaccharide through its long tail fibers
topic Applied Genetics and Molecular Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175313/
https://www.ncbi.nlm.nih.gov/pubmed/37133800
http://dx.doi.org/10.1007/s00253-023-12547-8
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