Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model

Introduction: In this paper we tried to conduct a novel nanomaterial strategy to overcome osteoarthritis (OA) in a mouse model. Methods: In this regard, after synthesizing the Mil-88a nanozyme, as a certain Fe-MOF, its toxic effects were detected by CCK-8 method and live-dead staining. The OA model...

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Autores principales: Hu, Hao, Huang, Xu, Dai, Yankun, Zhu, Kairun, Ye, Xuwen, Meng, Shengdong, Zhang, Qing, Xie, Xueguan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175628/
https://www.ncbi.nlm.nih.gov/pubmed/37187885
http://dx.doi.org/10.3389/fbioe.2023.1164942
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author Hu, Hao
Huang, Xu
Dai, Yankun
Zhu, Kairun
Ye, Xuwen
Meng, Shengdong
Zhang, Qing
Xie, Xueguan
author_facet Hu, Hao
Huang, Xu
Dai, Yankun
Zhu, Kairun
Ye, Xuwen
Meng, Shengdong
Zhang, Qing
Xie, Xueguan
author_sort Hu, Hao
collection PubMed
description Introduction: In this paper we tried to conduct a novel nanomaterial strategy to overcome osteoarthritis (OA) in a mouse model. Methods: In this regard, after synthesizing the Mil-88a nanozyme, as a certain Fe-MOF, its toxic effects were detected by CCK-8 method and live-dead staining. The OA model of mouse was constructed, and paraffin sections of joints were taken for histological evaluation. In addition, immunofluorescence and immunohistochemistry were used to identify the OA progression and OARSI was used to evaluate the OA grades. We observed that Mil-88a could be easily synthesized and has high biocompatibility. Results: We observed that Mil-88a could significantly promote the expression of OA anabolism-related genes such as Col2 and also significantly inhibit the expression of OA catabolism-related genes MMP13. Besides, we observed better OARSI score in animals treated with Mil-88a nano-enzyme loading on organic metal matrix. Discussion: Overall, Mil-88a nano-enzyme could be used as a novel strategy to treat OA.
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spelling pubmed-101756282023-05-13 Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model Hu, Hao Huang, Xu Dai, Yankun Zhu, Kairun Ye, Xuwen Meng, Shengdong Zhang, Qing Xie, Xueguan Front Bioeng Biotechnol Bioengineering and Biotechnology Introduction: In this paper we tried to conduct a novel nanomaterial strategy to overcome osteoarthritis (OA) in a mouse model. Methods: In this regard, after synthesizing the Mil-88a nanozyme, as a certain Fe-MOF, its toxic effects were detected by CCK-8 method and live-dead staining. The OA model of mouse was constructed, and paraffin sections of joints were taken for histological evaluation. In addition, immunofluorescence and immunohistochemistry were used to identify the OA progression and OARSI was used to evaluate the OA grades. We observed that Mil-88a could be easily synthesized and has high biocompatibility. Results: We observed that Mil-88a could significantly promote the expression of OA anabolism-related genes such as Col2 and also significantly inhibit the expression of OA catabolism-related genes MMP13. Besides, we observed better OARSI score in animals treated with Mil-88a nano-enzyme loading on organic metal matrix. Discussion: Overall, Mil-88a nano-enzyme could be used as a novel strategy to treat OA. Frontiers Media S.A. 2023-04-28 /pmc/articles/PMC10175628/ /pubmed/37187885 http://dx.doi.org/10.3389/fbioe.2023.1164942 Text en Copyright © 2023 Hu, Huang, Dai, Zhu, Ye, Meng, Zhang and Xie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Hu, Hao
Huang, Xu
Dai, Yankun
Zhu, Kairun
Ye, Xuwen
Meng, Shengdong
Zhang, Qing
Xie, Xueguan
Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model
title Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model
title_full Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model
title_fullStr Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model
title_full_unstemmed Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model
title_short Organic metal matrix Mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model
title_sort organic metal matrix mil-88a nano-enzyme for joint repair in the osteoarthritis mouse model
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175628/
https://www.ncbi.nlm.nih.gov/pubmed/37187885
http://dx.doi.org/10.3389/fbioe.2023.1164942
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