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Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)

Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat varieties. However, these gibberellic acid (GA)-insensitive...

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Autores principales: Zhang, Junli, Li, Chengxia, Zhang, Wenjun, Zhang, Xiaoqin, Mo, Youngjun, Tranquilli, Gabriela E., Vanzetti, Leonardo S., Dubcovsky, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175819/
https://www.ncbi.nlm.nih.gov/pubmed/37126674
http://dx.doi.org/10.1073/pnas.2300203120
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author Zhang, Junli
Li, Chengxia
Zhang, Wenjun
Zhang, Xiaoqin
Mo, Youngjun
Tranquilli, Gabriela E.
Vanzetti, Leonardo S.
Dubcovsky, Jorge
author_facet Zhang, Junli
Li, Chengxia
Zhang, Wenjun
Zhang, Xiaoqin
Mo, Youngjun
Tranquilli, Gabriela E.
Vanzetti, Leonardo S.
Dubcovsky, Jorge
author_sort Zhang, Junli
collection PubMed
description Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat varieties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its overexpression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties.
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spelling pubmed-101758192023-05-13 Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1) Zhang, Junli Li, Chengxia Zhang, Wenjun Zhang, Xiaoqin Mo, Youngjun Tranquilli, Gabriela E. Vanzetti, Leonardo S. Dubcovsky, Jorge Proc Natl Acad Sci U S A Biological Sciences Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat varieties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its overexpression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties. National Academy of Sciences 2023-05-01 2023-05-09 /pmc/articles/PMC10175819/ /pubmed/37126674 http://dx.doi.org/10.1073/pnas.2300203120 Text en Copyright © 2023 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by/4.0/This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biological Sciences
Zhang, Junli
Li, Chengxia
Zhang, Wenjun
Zhang, Xiaoqin
Mo, Youngjun
Tranquilli, Gabriela E.
Vanzetti, Leonardo S.
Dubcovsky, Jorge
Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_full Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_fullStr Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_full_unstemmed Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_short Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_sort wheat plant height locus rht25 encodes a platz transcription factor that interacts with della (rht1)
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175819/
https://www.ncbi.nlm.nih.gov/pubmed/37126674
http://dx.doi.org/10.1073/pnas.2300203120
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