Protocol to assess fatal embolism risks from human stem cells

Here, we present a protocol to identify the pro-embolic sub-population of human adipose-derived multipotent stromal cells (ADSCs) and predict fatal embolism risks from ADSC infusion. We describe steps for the collection, processing, and classification of ADSC single-cell RNA-seq data. We then detail...

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Detalles Bibliográficos
Autores principales: Ma, Fei, Zhang, Jinlai, Jin, Xin, Han, Pengfei, Liu, Yuling, Zhang, Ting, Yan, Kaijing, Kang, Y. James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176070/
https://www.ncbi.nlm.nih.gov/pubmed/37133989
http://dx.doi.org/10.1016/j.xpro.2023.102268
Descripción
Sumario:Here, we present a protocol to identify the pro-embolic sub-population of human adipose-derived multipotent stromal cells (ADSCs) and predict fatal embolism risks from ADSC infusion. We describe steps for the collection, processing, and classification of ADSC single-cell RNA-seq data. We then detail the development of a mathematical model for predicting ADSC embolic risk. This protocol allows for the development of prediction models to enhance the assessment of cell quality and advance the clinical applications of stem cells. For complete details on the use and execution of this protocol, please refer to Yan et al. (2022).(1)

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