E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT

X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a...

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Detalles Bibliográficos
Autores principales: Astanina, Elena, Petrillo, Sara, Genova, Tullio, Mussano, Federico, Bussolino, Federico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10176208/
https://www.ncbi.nlm.nih.gov/pubmed/37188107
http://dx.doi.org/10.21769/BioProtoc.4662
Descripción
Sumario:X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a method for 3D visualization and morphometric analysis by µCT scanning of the embryonic heart of iodine-stained E15.5 mouse embryos.

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